tRNA guanosine(15) transglycosylase TgtA
SEED: archaeosine tRNA-ribosyltransferase (EC 2.4.2.-) type 1 / archaeosine tRNA-ribosyltransferase (EC 2.4.2.-) PUA domain
KEGG: queuine tRNA-ribosyltransferase ; conserved protein with predicted RNA binding PUA domain
Top 20 experiments with the strongest phenotypes (|fitness| ≥ 1.2)
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group | condition | fitness | t score | |
nutrient t2 | H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE | -2.4 | -13.3 | compare |
mixed community | Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse3; 20 generations | -1.8 | -5.9 | compare |
nutrient t1 | H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE | -1.5 | -11.0 | compare |
stress | McCas_H2_complete Calcium Chloride Dihydrate 83.3333333333333 mM | -1.5 | -7.6 | compare |
mixed community | Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; 1,3-Propanediol_timecourse1; 20 generations | -1.5 | -11.8 | compare |
stress | McCas_formate_complete selenate 31.25 mM | -1.5 | -11.3 | compare |
nutrient t2 | Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE | -1.4 | -10.7 | compare |
mixed community | Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanol_timecourse3; 20 generations | -1.4 | -10.6 | compare |
mixed community | Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse1; 20 generations | -1.3 | -7.7 | compare |
mixed community | Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanolamine_timecourse2; 20 generations | -1.3 | -5.7 | compare |
mixed community | Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanol_timecourse2; 20 generations | -1.3 | -10.6 | compare |
nutrient t2 | H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE | -1.3 | -6.2 | compare |
mixed community | Mixed culture; Syntrophotalea carbinolica 1:1 starting OD to methanogen; Ethanol_timecourse1; 20 generations | -1.2 | -10.3 | compare |
stress | McCas_formate_complete Econazole nitrate salt 0.02 mM | -1.2 | -6.6 | compare |
stress | McCas_formate_complete Phenazine methosulfate 0.0333333333333333 mM | -1.2 | -5.9 | compare |
stress | McCas_formate_complete 5-Fluorouracil 4.16666666666667E-05 mM | -1.2 | -6.3 | compare |
nitrogen source | H2 N-free with 10mM NAG | +1.3 | +6.6 | compare |
nitrogen source | H2 N-free with 10mM Urea | +1.5 | +8.5 | compare |
nitrogen source | H2 N-free with 10mM L-tyrosine | +2.2 | +9.5 | compare |
nitrogen source | H2 N-free with 10mM L-tyrosine | +2.3 | +9.6 | compare |