7-carboxy-7-deazaguanine synthase QueE
SEED: Queuosine Biosynthesis QueE Radical SAM
Top 20 experiments with the strongest phenotypes (|fitness| ≥ 0.5)
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| group | condition | fitness | t score | |
|---|
| rich t1 | H2 McCas, 5g/L bicarb, 40 psi H2CO2 | -0.7 | -4.6 | compare |
| nutrient t2 | H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE | -0.7 | -4.4 | compare |
| nitrogen source | H2 N-free with 2.5mM Cytosine | -0.6 | -2.2 | compare |
| stress | Formate 100 mM hypophosphite | -0.6 | -3.2 | compare |
| nutrient t1 | H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE | -0.5 | -4.1 | compare |
| stress | McCas_formate_complete 5-Fluorouracil 4.16666666666667E-05 mM | -0.5 | -2.7 | compare |
| stress | McCas_H2_complete Antimycin A 0.01 mM | -0.5 | -2.4 | compare |
| nutrient t1 | Mc formate, casamino free, with 10mM L-Asparagine | -0.5 | -3.3 | compare |
| nutrient t1 | Mc formate, casamino free, with 10mM L-Methionine | -0.5 | -2.0 | compare |
| stress | McCas_formate_complete Rifampicin 0.5 mM | -0.5 | -2.1 | compare |
| no stress control | McCas_formate_complete no stress control | +0.5 | +2.3 | compare |
| natural competence | H2 McCas- cells incubated with 5000 ng DNA (pDNA pLW40neo) then transferred and grown in liquid medium with neomycin | +0.5 | +2.2 | compare |
| stress | H2 50 mM hypophosphite | +0.5 | +2.7 | compare |
| stress | McCas_H2_complete metavanadate 4.54545454545455 mM | +0.5 | +1.4 | compare |
| stress | McCas_H2_complete potassium hexahydroxoantimonate 0.125 mM | +0.6 | +1.9 | compare |
| nitrogen source | H2 N-free with 10mM L-tyrosine | +0.6 | +2.6 | compare |
| nitrogen source | H2 N-free with 10mM NAG | +0.6 | +3.4 | compare |
| stress | McCas_H2_complete Chlorate 250 mM | +0.7 | +2.5 | compare |
| stress | McCas_H2_complete Cesium chloride 41.6666666666667 mM | +0.8 | +2.4 | compare |
| nitrogen source | H2 N-free with 10mM L-tyrosine | +0.9 | +3.2 | compare |