| group | condition | fitness | t score | |
| rich t1 | H2 McCas, 5g/L bicarb, 40 psi H2CO2 | -5.2 | -3.2 | compare |
| attachment assay; attached sample | McCas-formate glass attached | -4.9 | -1.1 | compare |
| nutrient t2 | H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE | -4.9 | -2.2 | compare |
| nitrogen source | H2 N-free with 2g/L casaminos 10mM Ammonium chloride | -4.8 | -2.1 | compare |
| attachment assay; attached sample | McCas-formate glass attached | -4.8 | -1.2 | compare |
| attachment assay; attached sample | McCas-formate glass attached | -4.8 | -1.4 | compare |
| rich t2 | H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE | -4.7 | -2.0 | compare |
| rich t2 | H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 | -4.7 | -1.9 | compare |
| plankonic | Formate pooled triplicate planktonic cells | -4.6 | -2.8 | compare |
| iron | Iron excess (2.5x) , H2 media | -4.5 | -1.4 | compare |
| nutrient t2 | H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE | -4.5 | -1.4 | compare |
| nutrient t2 | H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE | -4.5 | -1.5 | compare |
| rich t2 | H2 McCas, 5g/L bicarb, 40 psi H2CO2 | -4.3 | -1.2 | compare |
| iron | control, 1x iron with casaminos and vitamins, formate media | -4.1 | -3.2 | compare |
| rich t1 | H2 McCas, 5g/L bicarb, 40 psi H2CO2 | -4.0 | -3.5 | compare |
| nutrient t1 | formate McAA riboflavin dropout | -4.0 | -3.1 | compare |
| nutrient t2 | Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE | -4.0 | -2.0 | compare |
| nitrogen source | H2 N-free with 10mM Ammonium chloride | -3.9 | -2.0 | compare |
| rich t1 | Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE | -3.9 | -3.3 | compare |
| nutrient t1 | Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE | -3.9 | -2.4 | compare |