| group | condition | fitness | t score | |
| nutrient t2 | H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE | -4.6 | -6.2 | compare |
| rich t2 | H2 McCas, 5g/L bicarb, 40 psi H2CO2 | -4.5 | -4.6 | compare |
| nutrient t2 | H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE | -3.9 | -6.8 | compare |
| nutrient t2 | H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 Vitamin & Casamino Acid FREE | -3.8 | -5.8 | compare |
| rich t2 | H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE | -3.8 | -5.6 | compare |
| rich t2 | H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE | -3.8 | -6.1 | compare |
| nutrient t2 | H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 Vitamin & Casamino Acid FREE | -3.8 | -5.5 | compare |
| nutrient t2 | Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE | -3.8 | -5.2 | compare |
| nutrient t2 | H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE | -3.7 | -5.4 | compare |
| nutrient t2 | Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Vitamin FREE | -3.7 | -5.3 | compare |
| rich t2 | H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 | -3.5 | -7.4 | compare |
| rich t2 | Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE | -3.5 | -8.3 | compare |
| nitrogen source | H2 N-free with 2g/L casaminos 10mM Ammonium chloride | -3.4 | -6.2 | compare |
| nitrogen source | Formate N-free with 2mM Adenosine | -3.3 | -3.8 | compare |
| stress | Formate 50 mM hypophosphite | -3.2 | -6.8 | compare |
| iron | control, 1x iron with casaminos and vitamins, H2 media | -3.2 | -7.4 | compare |
| nutrient t2 | Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE | -3.2 | -6.8 | compare |
| rich t2 | Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE | -3.2 | -7.2 | compare |
| nitrogen source | H2 N-free with 2g/L casaminos 10mM Ammonium chloride | -3.2 | -6.0 | compare |
| nitrogen source | Formate N-free with 2mM Adenosine | -3.2 | -3.3 | compare |