bifunctional N(6)-L-threonylcarbamoyladenine synthase/serine/threonine protein kinase
SEED: TsaD/Kae1/Qri7 protein, required for threonylcarbamoyladenosine t(6)A37 formation in tRNA / p53-regulating protein kinase (human PRPK/yeast YGR262c)
KEGG: O-sialoglycoprotein endopeptidase ; Mn2+-dependent serine/threonine protein kinase
Top 20 experiments with the strongest phenotypes (|fitness| ≥ 3.7)
Or see all 217 experiments or choose conditions
| group | condition | fitness | t score | |
|---|
| nutrient t1 | formate McAA p-aminobenzoic dropout | -4.4 | -3.0 | compare |
| nitrogen source | H2 N-free with 2g/L casaminos | -4.4 | -2.7 | compare |
| nitrogen source | H2 N-free with 2g/L casaminos 10mM Ammonium chloride | -4.2 | -2.5 | compare |
| nutrient t2 | H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE | -4.2 | -2.6 | compare |
| iron | Iron excess (2.5x) , H2 media | -4.2 | -2.3 | compare |
| iron | Iron excess (2.5x) , H2 media | -4.2 | -1.8 | compare |
| iron | control, 1x iron with casaminos and vitamins, H2 media | -4.2 | -2.7 | compare |
| rich t2 | H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE | -4.1 | -2.4 | compare |
| nutrient t1 | formate McAA pyroxidine HCl dropout | -4.0 | -3.5 | compare |
| nutrient t2 | H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE | -3.9 | -1.7 | compare |
| nutrient t2 | H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE | -3.8 | -1.7 | compare |
| nutrient t1 | formate McAA riboflavin dropout | -3.8 | -3.7 | compare |
| nutrient t1 | formate vitamin complete | -3.8 | -3.7 | compare |
| no stress control | McCas_formate_complete no stress control | -3.8 | -1.6 | compare |
| rich t2 | H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 | -3.8 | -2.1 | compare |
| nutrient t1 | H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE | -3.8 | -3.6 | compare |
| stress | McCas_formate_complete Spiramycin 0.1 mM | -3.7 | -1.4 | compare |
| stress | McCas_formate_complete Sulfaquinoxaline 0.1 mM | -3.7 | -1.4 | compare |
| nitrogen source | H2 N-free with 10mM Ammonium chloride | -3.7 | -2.9 | compare |
| nitrogen source | H2 N-free with 2g/L casaminos 10mM Ammonium chloride | -3.7 | -3.3 | compare |