| group | condition | fitness | t score | |
| nutrient t1 | H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE | -6.0 | -4.1 | compare |
| nitrogen source | H2 N-free with 2g/L casaminos 10mM Ammonium chloride | -6.0 | -3.5 | compare |
| iron | control, 1x iron with casaminos and vitamins, formate media | -6.0 | -4.1 | compare |
| nutrient t1 | H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE | -6.0 | -4.1 | compare |
| rich t2 | H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 | -5.9 | -3.6 | compare |
| nutrient t2 | H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE | -5.9 | -3.4 | compare |
| nitrogen source | H2 N-free with 2g/L casaminos | -5.8 | -3.3 | compare |
| iron | Iron excess (2.5x) , H2 media | -5.8 | -3.0 | compare |
| iron | Iron excess (2.5x) , H2 media | -5.8 | -3.0 | compare |
| nitrogen source | H2 N-free with 10mM Ammonium chloride | -5.7 | -3.1 | compare |
| rich t2 | H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE | -5.7 | -3.1 | compare |
| rich t2 | H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE | -5.7 | -3.1 | compare |
| rich t2 | H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 | -5.7 | -3.0 | compare |
| attachment assay; attached sample | McCas-formate glass attached | -5.6 | -2.1 | compare |
| attachment assay; attached sample | McCas-formate glass attached | -5.6 | -1.9 | compare |
| attachment assay; attached sample | McCas-formate glass attached | -5.6 | -2.1 | compare |
| nutrient t2 | H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE | -5.6 | -2.8 | compare |
| nutrient t2 | H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE | -5.5 | -2.3 | compare |
| nutrient t2 | H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE | -5.5 | -2.4 | compare |
| rich t2 | H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 | -5.5 | -2.8 | compare |