tRNA guanosine(15) transglycosylase TgtA
SEED: archaeosine tRNA-ribosyltransferase (EC 2.4.2.-) type 1 / archaeosine tRNA-ribosyltransferase (EC 2.4.2.-) PUA domain
KEGG: queuine tRNA-ribosyltransferase ; conserved protein with predicted RNA binding PUA domain
Top 20 experiments with the strongest phenotypes (|fitness| ≥ 2.2)
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| group | condition | fitness | t score | |
| planktonic | H2 pooled triplicate planktonic cells | -3.4 | -14.4 | compare |
| rich t2 | Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE | -2.8 | -14.8 | compare |
| nitrogen source | H2 N-free with 2g/L casaminos 10mM Ammonium chloride | -2.8 | -13.8 | compare |
| rich t2 | H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE | -2.8 | -12.9 | compare |
| biofilm | H2 pooled triplicate biofilm cells | -2.8 | -13.6 | compare |
| rich t2 | H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 | -2.7 | -14.3 | compare |
| rich t2 | Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE | -2.6 | -14.1 | compare |
| rich t2 | H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE | -2.5 | -13.4 | compare |
| rich t2 | Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE | -2.5 | -14.0 | compare |
| rich t2 | H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2 | -2.5 | -13.5 | compare |
| nutrient t2 | H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE | -2.4 | -10.4 | compare |
| rich t2 | H2 McCas, 5g/L bicarb, 40 psi H2CO2 | -2.3 | -9.8 | compare |
| nutrient t2 | H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE | -2.3 | -10.2 | compare |
| nutrient t2 | H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE | -2.3 | -11.1 | compare |
| iron | control, 1x iron with casaminos and vitamins, H2 media | -2.2 | -11.5 | compare |
| iron | Iron excess (2.5x) , H2 media | -2.2 | -9.5 | compare |
| rich t2 | H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE | -2.2 | -12.9 | compare |
| iron | control, 1x iron with casaminos and vitamins, H2 media | -2.2 | -11.2 | compare |
| nitrogen source | H2 N-free with 2g/L casaminos | -2.2 | -12.2 | compare |
| iron | Iron limitation (0.2x), H2 media | -2.2 | -12.2 | compare |