| group | condition | fitness | t score | |
| rich t2 | H2 McCas, 5g/L bicarb, 40 psi H2CO2 | -1.5 | -5.6 | compare |
| nutrient t2 | H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 Vitamin & Casamino Acid FREE | -1.3 | -7.2 | compare |
| nutrient t2 | H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE | -1.3 | -7.2 | compare |
| nutrient t2 | H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 Vitamin & Casamino Acid FREE | -1.3 | -7.1 | compare |
| nutrient t2 | H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE | -1.2 | -6.8 | compare |
| nutrient t2 | H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE | -1.2 | -7.1 | compare |
| nitrogen source | H2 N-free with 2mM Adenosine | -1.1 | -5.5 | compare |
| nitrogen source | H2 N-free with 10mM Ammonium chloride | -1.1 | -6.3 | compare |
| nitrogen source | Formate N-free with 2mM Adenosine | -1.1 | -5.1 | compare |
| nutrient t2 | H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE | -1.0 | -6.7 | compare |
| nutrient t1 | H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 Vitamin & Casamino Acid FREE | -1.0 | -7.1 | compare |
| nutrient t2 | H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Vitamin FREE | -1.0 | -6.4 | compare |
| nutrient t2 | Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE | -1.0 | -5.6 | compare |
| rich t2 | H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE | -1.0 | -5.6 | compare |
| nitrogen source | Formate N-free with 2mM Adenosine | -1.0 | -4.5 | compare |
| nitrogen source | Formate N-free with 2mM Adenosine | -0.9 | -4.1 | compare |
| iron | Iron excess (2.5x) , H2 media | -0.9 | -6.6 | compare |
| nitrogen source | H2 N-free with 2mM Adenosine | -0.9 | -4.6 | compare |
| nitrogen source | H2 N-free with 10mM D-glucosamine | -0.9 | -5.5 | compare |
| nitrogen source | H2 N-free with 10mM Ammonium chloride | -0.9 | -5.5 | compare |