Pairwise Alignments
Query, 1465 a.a., Rhs-family protein from Pseudomonas fluorescens FW300-N2E2
Subject, 1411 a.a., Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor from Escherichia coli BL21
Score = 254 bits (648), Expect = 6e-71
Identities = 264/981 (26%), Positives = 386/981 (39%), Gaps = 173/981 (17%)
Query: 484 LVSYAYDAANTLAEVRDATGQVQRRFRYDAEQR--MVEHQLPSGLHCFYEWALIDDQEWR 541
LV Y + LA V D + R F YD + R MV H+ Y + D + R
Sbjct: 311 LVRYGWTPRGELAAVYDRSNTQVRSFTYDDKYRGRMVAHRHTGRPEIRYRY----DSDGR 366
Query: 542 VVRHWTDEGDAYQFDYDLKAGITRISDSLQRVSTRHWNSQ---HQITQYTDNLGQTWLFE 598
V G +Y + Y+ K IT I+DSL R H + ++ + G +
Sbjct: 367 VTEQLNPAGLSYTYQYE-KDHIT-ITDSLDRREVLHTQGEAGLKRVVKKEHADGSVTQSQ 424
Query: 599 WNDERQMLSATDPQGGQYQFNYDDAGNLIGE-TDPLGRSESTLWLEHWALPLVDTNAAGH 657
++ ++ + TD G +++ D LI T P GR+ + + H L T G
Sbjct: 425 FDAVGRLRTQTDAAGRTTEYSPDVVTGLITRITTPDGRASAFYYNHHNQLTSA-TGPDGL 483
Query: 658 SWKYRYDQRGNCTTEIDPLGHVTRYRYD-AHGQV-VEIIDATGKNKKLRWNPFGQLVEHV 715
+ YD+ G E P G +TRYRYD H + DATG K + W+ +GQL+
Sbjct: 484 ELRREYDELGRLIQETAPDGDITRYRYDNPHSDLPCATEDATGSRKTMTWSRYGQLLSFT 543
Query: 716 DCSGYPTRFSYDARGYLHTITDALGERTQFSYDAQGRLLGNQLPDGRTEHYQRDTAGLLI 775
DCSGY TR+ +D G + + G +YD++G+L+ + G Y+ + AG L
Sbjct: 544 DCSGYVTRYDHDRFGQMTAVHREEGLSQYRAYDSRGQLIAVKDTQGHETRYEYNIAGDLT 603
Query: 776 GYTDPAGHTTLYQHNRRGQVRQRTDAQGRQVQFTYDSFGRLQALTNENGESYRFAWDAGD 835
P G Q++ G+ RT G YD+ GR+ LT+ENG F +D D
Sbjct: 604 AVIAPDGSRNGTQYDAWGKA-VRTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYDVLD 662
Query: 836 RLTEQQDLDGSAKRYDYDRLDNVAAVTAMPAPYGTGLALIPETPPAPIVHRLERDAVGRL 895
RL ++ DG +RY +D TG + E +V D RL
Sbjct: 663 RLIQETGFDGRTQRYHHDL---------------TGKLIRSEDEG--LVTHWHYDEADRL 705
Query: 896 IAKVTDDGRTE-YSYDPLDQLTAVTFTDLQGNAQTLSFAYDALGQLLAENSTAG------ 948
+ E + YD LT ++ +G+ + + YD G+L E T
Sbjct: 706 THRTVKGETAERWQYDERGWLTDISHIS-EGHRVAVHYRYDEKGRLTGERQTVHHPETEA 764
Query: 949 -----SLQHHYDELG---NLIQSQLPDGRWVNRLYYGSGHLHQINLDGKVISDFERDRLH 1000
+H Y+ G I LP W L YGSG L + L + ++ RDRLH
Sbjct: 765 LLWQHETRHAYNAQGLANRCIPDSLPAVEW---LTYGSGWLSGMKLGDTPLVEYTRDRLH 821
Query: 1001 REVLRTQGQISTRSEYDRSGRLRTRQ---------------------------------- 1026
RE LR+ G+ + Y +G+L+++
Sbjct: 822 RETLRSFGRYELSTAYTPAGQLQSQHLNSLQYDRDYTWNDNGELIRISSPRQTRSYSYST 881
Query: 1027 --RRHSGQPSLLPAAVQKHFEYDPADNLIGKLDQQP------------AAQQRQLLHYDA 1072
R S + ++ + DPA N + + P A L YD
Sbjct: 882 TGRLTSVHTTAANLDIRIPYATDPAGNRLPDPELHPDSTLSMWPDNRIARDAHYLYRYDR 941
Query: 1073 TGRIIASQDSL--------HGQRETFTYDAAANLLDGPAPGAGLVVHNKLLTYQD----K 1120
GR+ D + + + YD+ L VH Y +
Sbjct: 942 YGRLTEKTDLIPEGVIRTDDERTHQYHYDSQHRL-----------VHYTRTQYAEPLVES 990
Query: 1121 RYRYDAFGRMIEKR--------------SAKRGVQHFAYDAESRLIEVRNDDGSVVNM-- 1164
RY YD GR + KR S K V + +D + RL ++ND + +
Sbjct: 991 RYLYDPLGRRVAKRVWRRERDLTGWMSLSRKPEVTWYGWDGD-RLTTIQNDRTRIQTIYQ 1049
Query: 1165 --AYDPL--------------GRRIAKTEHDNHGYPLGETRFT------WDGL------- 1195
++ PL R +A T + G G F D L
Sbjct: 1050 PGSFTPLIRVETATGELAKTQRRSLADTLQQSGGEDGGSVVFPPVLVQMLDRLESEILAD 1109
Query: 1196 RLLQEHRHQQTSLYLYEDDGYEPLARVDGSGPLQKIRYYHNDLNGLPEQLTEADGHNVWQ 1255
R+ +E R S L + + V P +KI YH D GLP L +G W
Sbjct: 1110 RVSEESRRWLASCGLTVEQMQNQMDPV--YTPARKIHLYHCDHRGLPLALISTEGATAWC 1167
Query: 1256 ATYRVWGNTLEEVREPYYIEEQNLRFQGQYLDRETGLHFNTFRFYDPDVGRFTTPDPIGL 1315
A Y WGN L E P+ ++ Q +R GQ D E+GL++N R+YDP GR+ T DPIGL
Sbjct: 1168 AEYDEWGNLLNE-ENPHQLQ-QLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGL 1225
Query: 1316 AGGANLYQYAPNPLGWIDPWG 1336
GG NLY Y NP+ IDP G
Sbjct: 1226 KGGWNLYGYQLNPISDIDPLG 1246
Score = 47.4 bits (111), Expect = 1e-08
Identities = 46/163 (28%), Positives = 75/163 (46%), Gaps = 18/163 (11%)
Query: 272 LVGGAVSMAASSAANAAANAAMG---SSNPVHAATGAKVLGADEDLDFVLPGILPIDWQR 328
+V G+ + + A + G S +PV+ GAKVL + D+ LP LP R
Sbjct: 19 IVQGSAGVRIGAPTGVACSVCPGGVTSGHPVNPLLGAKVLPGETDI--ALPAPLPFILSR 76
Query: 329 VYNSHDERRDS---LFGAGWSVAYEVQVQIQPHPEGGETLIYTDEQGRPIDMGSIPLGGA 385
Y+S+ + + G GW + ++++Q++ + TLI +D GR + + G
Sbjct: 77 TYSSYRTKTPAPVGSLGPGWKMPADIRLQLRDN-----TLILSDNGGRSLYFEHLFPGED 131
Query: 386 VFSAGEGLAVRRHVNGQLLIESEDGLYRLFEPSPANPSHLRLS 428
+S E L + V G +L +E +RL A P LRLS
Sbjct: 132 GYSRSESLWL---VRGGVLRLNEG--HRLAALWQALPEELRLS 169
Score = 35.8 bits (81), Expect = 3e-05
Identities = 91/409 (22%), Positives = 146/409 (35%), Gaps = 100/409 (24%)
Query: 440 LDYDDAGRLVRLRDTFDLVHVELIRAGERVTHIERLYPDQSREVLVSYAYDAANTLAEVR 499
++YD AGR++RL E +H ++ YD + L +
Sbjct: 635 MEYDAAGRVIRL-------------TSENGSH-------------TTFRYDVLDRLIQET 668
Query: 500 DATGQVQRRFRYDAEQRMVEHQLPSGLHCFYEWALIDDQEWRVVRHWTDEGDAYQFDYDL 559
G+ Q R+ +D +++ + GL + + D R V+ T E ++ YD
Sbjct: 669 GFDGRTQ-RYHHDLTGKLIRSE-DEGLVTHWHYDEADRLTHRTVKGETAE----RWQYDE 722
Query: 560 KAGITRISDSLQRVSTRHWNSQHQITQY--TDNLGQTWLFEWNDERQMLSATDPQGGQYQ 617
+ +T IS H + H++ + D G+ ERQ + + + +Q
Sbjct: 723 RGWLTDIS---------HISEGHRVAVHYRYDEKGRL-----TGERQTVHHPETEALLWQ 768
Query: 618 FNYDDAGNLIGET-----DPLGRSE----STLWLEHWAL---PLVD-TNAAGHSWKYRYD 664
A N G D L E + WL L PLV+ T H R
Sbjct: 769 HETRHAYNAQGLANRCIPDSLPAVEWLTYGSGWLSGMKLGDTPLVEYTRDRLHRETLRSF 828
Query: 665 QRGNCTTEIDPLG-----HVTRYRYDAHGQVVEIIDATGKNKKLRWNPFGQLVEHVDCSG 719
R +T P G H+ +YD + WN G+L+ +
Sbjct: 829 GRYELSTAYTPAGQLQSQHLNSLQYD---------------RDYTWNDNGELI-RISSPR 872
Query: 720 YPTRFSYDARGYL---HTITDALGERTQFSYDAQGRLLGNQLPDGRTEHYQRDTAGLLIG 776
+SY G L HT L R ++ D GN+LPD E + T +
Sbjct: 873 QTRSYSYSTTGRLTSVHTTAANLDIRIPYATDP----AGNRLPD--PELHPDSTLSMWPD 926
Query: 777 YTDPAGHTTLYQHNRRGQVRQRTDA---------QGRQVQFTYDSFGRL 816
LY+++R G++ ++TD R Q+ YDS RL
Sbjct: 927 NRIARDAHYLYRYDRYGRLTEKTDLIPEGVIRTDDERTHQYHYDSQHRL 975