Pairwise Alignments

Query, 452 a.a., glutamine synthetase from Pseudomonas fluorescens FW300-N2E3

Subject, 472 a.a., glutamate--putrescine ligase from Escherichia coli BL21

 Score =  230 bits (587), Expect = 6e-65
 Identities = 154/452 (34%), Positives = 233/452 (51%), Gaps = 17/452 (3%)

Query: 7   QLSSWLKEHKITE-VECVVSDLTGIARGKIAPTNKFLH-ERGMRLPESVLLQTVTGDFVD 64
           ++  +L+ +  T+ V+ +++DL G  RGK  P       E+G   P SV    + G+ V+
Sbjct: 26  EVKRYLERYPNTQYVDVLLTDLNGCFRGKRIPVASLKKLEKGCYFPASVFAMDILGNVVE 85

Query: 65  D-DIYYDLLDPADIDMVCRPDSSAIYVVPWAIEPTAI--VIHDTFDKLGNPIELSPRNVL 121
           +  +  ++ +P   D  C P   ++   P A +P  I  ++    D+ G P ++ PRNVL
Sbjct: 86  EAGLGQEMGEP---DRTCVPVLGSL--TPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVL 140

Query: 122 KKVLKLYADKGWQPIVAPEMEFYLTQRCEDPDLPLKAPLGRSGRAESGRQS--FSIDAAN 179
            ++ +    +G  P+VA E+EFYL  R  D +  L+ P    G  +   QS  +S+D  N
Sbjct: 141 NRLWQQLRQRGLFPVVAVELEFYLLDRQRDAEGYLQPPCA-PGTDDRNTQSQVYSVDNLN 199

Query: 180 EFDPLFEDVYDWCELQGLDLDTLIHEDGPAQMEINFRHGD-ALDLADQITVFKRTLREAA 238
            F  +  D+ +  +LQ +  D  + E  P Q EIN  H D  L+  D     KR +R  A
Sbjct: 200 HFADVLNDIDELAQLQLIPADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMA 259

Query: 239 LKHNVTATFMAKPIGDEPGSAMHLHQSVVDIATGKPIFADSDGQMSELFLHHIGGLQKYI 298
            KH + ATFMAKP  +  GS MH+H S+ +   G+ + +D++G+ S L    + G+   +
Sbjct: 260 EKHKMHATFMAKPYEEHAGSGMHIHISMQN-NRGENVLSDAEGEDSPLLKKMLAGMIDLM 318

Query: 299 PKLLPMFAPNVNSFRRFLPDTSAPVNVEWGEENRTVGLRVPTSGPEAMRVENRLPGADAN 358
           P  + + APNVNS+RRF P    P    WG  NRTV LR+P       RVE R+ GADAN
Sbjct: 319 PSSMALLAPNVNSYRRFQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADAN 378

Query: 359 PYLAIAASLLCGYL-GMIERIEPSAPVQGRAYERRNLRLPITIEDALAHMEDCPTIERYL 417
           PYL +AA +  G L G+   +     V+G   E+  L  PI   DAL    +   + RYL
Sbjct: 379 PYLVMAA-IFAGILHGLDNELPLQEEVEGNGLEQDGLPFPIRQSDALGEFIENDHLRRYL 437

Query: 418 GAKFVRGYVAVKRAEHENFKRVISSWEREFLL 449
           G +F   Y A K  E   F+R+I+  E E++L
Sbjct: 438 GERFCHVYHACKNDELLQFERLITETEIEWML 469