Pairwise Alignments
Query, 1096 a.a., histidine kinase from Pseudomonas fluorescens FW300-N2E3
Subject, 1247 a.a., diguanylate cyclase from Pseudomonas simiae WCS417
Score = 480 bits (1236), Expect = e-139
Identities = 272/692 (39%), Positives = 403/692 (58%), Gaps = 19/692 (2%)
Query: 400 DITDQVEREQRLITSEEKFASLFQVSPDPICVTRQDTGQFIEINPAFTQIFGWTTTDVID 459
DIT E +Q+L TSEEKFA F SPD + ++RQ G IE+N F++I G+T+ +D
Sbjct: 545 DITQLKETQQQLQTSEEKFAKAFHASPDGLLLSRQRDGLLIEVNDGFSRITGFTSATSLD 604
Query: 460 RSADEIGLWDASVKRQQRIEQVIREQALNNVAIIVHHKDGQPLTCVISSRQISVGNEPCI 519
+S ++G+W +R+ +E + R+ + + + DGQ C +SSR + +G++ C+
Sbjct: 605 QSTLDLGIWVDLNERKHMLELMQRDGFVRDFICHIRRSDGQIRLCELSSRPLPIGDDDCM 664
Query: 520 VTTLRDITQQQRSEAALKASEEKFAKAFHSSPDAITITERDSGRYLEVNDGFCRLTGYSA 579
+T RDIT++Q + L+ + A F S+ + + IT+ VN F +TGYS
Sbjct: 665 LTIARDITERQLMQEKLQQA----ATVFESTAEGVLITDTRQN-ISAVNRAFSEITGYSE 719
Query: 580 DEVVGKTVYQIGIWAEEKQ-RSTLLAALKAKGRVHHQ-EMLGRNKRGEILTVEVSIEPIT 637
E +G T + + + + L A+G H Q E+ R K GE+ ++I +
Sbjct: 720 AEALGNTPRLLASGLHDSAFYAAMWHQLTAQG--HWQGEISNRRKNGELYPSWLTISAVR 777
Query: 638 LNETACLLMTA--RDVSLLKNAEAQIRHLAYHDPLTNLPNRALLMDRLSQQIALLKRHNL 695
E A D+S LK A+A++ + A+HDPLT LPNR L RL + +
Sbjct: 778 NREQLITHFVAVFADISSLKLAQARLDYQAHHDPLTGLPNRTLFESRLQAALNGHQETGQ 837
Query: 696 RGALLFLDLDHFKHINDSLGHPVGDTVLKIITARLEASVRMEDTVARLGGDEFVVLLSGL 755
+GA+LFLDLD FKHINDSLGHP+GD +LK I RL+ +R DTVARLGGDEF++LL GL
Sbjct: 838 QGAVLFLDLDRFKHINDSLGHPIGDLLLKDIAVRLKEQLRDIDTVARLGGDEFIILLPGL 897
Query: 756 EGTRNEVSEQVRELADTLRELLSEPMFLDGQRLQVTPSIGVALIPDHGSTPTDLLKRADI 815
+ + + LA+ L + P ++ SIG +L P+ G+ L+K AD
Sbjct: 898 QH-----ASDAQYLANKLLACFTPPFQAGEHEFFISASIGTSLYPEDGTDVATLVKNADA 952
Query: 816 ALYRAKDSGRNATQMFHNTMQKAASERLRMETDLRLALSRGEFSVQYQPQVDALDGRIIG 875
A+YR+K GRN + + + A+ER+ +E +LR A+ R E S+ YQP++ +IG
Sbjct: 953 AMYRSKAKGRNRVESYTRDLTAQANERVALEHELRRAIERDELSLYYQPKLSLATHELIG 1012
Query: 876 AEALVRWRHPRLGEQSPSEFIKVLEDSGLILEVGAWILDETCQAFRQLIDRGLIDPLNFS 935
AEAL+RW HP G+ P FI + E++G+IL++G W+L++ C+ +G D
Sbjct: 1013 AEALIRWHHPTFGDVPPEHFIALAEENGMILQIGDWVLEQACRQMHAW--QGHFDDFG-P 1069
Query: 936 LCVNISPRQFRQNDFVERIEQTLSSHGLPYSLLKLEVTEGIVIQNLDDTISKMRRLKKLG 995
L VN++ Q R + RI+Q L + L L+LE+TE ++ ++ + + +LK LG
Sbjct: 1070 LSVNLAGAQLRHPGLLGRIKQLLHDYQLDPGRLQLEITENFIMSQAEEALDVLHQLKDLG 1129
Query: 996 VSFAMDDFGTGYSSLTYLKRLPVDTLKIDQSFIRDATSDPNDAEIIRAIVAMARSLELTV 1055
V A+DDFGTGYSSL+YLKRLP+D LKIDQSF+R DP+DA I+RAI+A+ S++ T+
Sbjct: 1130 VQLAIDDFGTGYSSLSYLKRLPLDFLKIDQSFVRGLPDDPHDAAIVRAIIALGHSMQFTI 1189
Query: 1056 IAEGVETTEQLDFLQGLGCHLYQGYLHSQPLP 1087
IAEGVE Q FL GC QGY+ S PLP
Sbjct: 1190 IAEGVENPAQQAFLAAEGCEQMQGYIVSLPLP 1221
Score = 164 bits (415), Expect = 4e-44
Identities = 109/385 (28%), Positives = 187/385 (48%), Gaps = 10/385 (2%)
Query: 271 DTSERKLAEQALKDSQERLDLALDSAQLGTWDWHIPSGML-YGSARAAQLHGLDPIPFHE 329
+ + R EQ L+ S+ ++S W+ + Y S A +L G H
Sbjct: 295 EIARRVALEQELRSSEYHYRGLVESLSAIAWEASVADFTYSYVSPHAEELLGYPRA--HW 352
Query: 330 PFDAFFEGV--PDDERDSMRDAYRSLREGPAGNYQLTYRVQLPDGSSRYLESRARLYRDE 387
F+ + P D + YR R ++ + YRV DG ++ L E
Sbjct: 353 LIPGFWRNIIHPADLTRAEAYCYRETRANR--DHSIDYRVITADGRCLWVRDIVSLI--E 408
Query: 388 SGVPLRMAGTLLDITDQVEREQRLITSEEKFASLFQVSPDPICVTRQDTGQFIEINPAFT 447
G + G ++DI++ E+ L SE+KFAS+FQ PD + + R G +E+N AF
Sbjct: 409 HGHEPVLRGLMIDISEAKRTEEALQLSEQKFASVFQQCPDMLVIARLSDGCLLEVNKAFE 468
Query: 448 QIFGWTTTDVIDRSADEIGLWDASVKRQQRIEQVIREQALNNVAIIVHHKDGQPLTCVIS 507
G V+ ++A E+ +W +++V + ++ N+ + +GQ T +IS
Sbjct: 469 DQIGLKAEHVVGKTATELNIWGIQGVGPDLLQRV-QTTSIRNLEMPFLRSNGQAFTGLIS 527
Query: 508 SRQISVGNEPCIVTTLRDITQQQRSEAALKASEEKFAKAFHSSPDAITITERDSGRYLEV 567
+ + IV +RDITQ + ++ L+ SEEKFAKAFH+SPD + ++ + G +EV
Sbjct: 528 AEPFQLDTIEAIVVVVRDITQLKETQQQLQTSEEKFAKAFHASPDGLLLSRQRDGLLIEV 587
Query: 568 NDGFCRLTGYSADEVVGKTVYQIGIWAEEKQRSTLLAALKAKGRVHHQEMLGRNKRGEIL 627
NDGF R+TG+++ + ++ +GIW + +R +L ++ G V R G+I
Sbjct: 588 NDGFSRITGFTSATSLDQSTLDLGIWVDLNERKHMLELMQRDGFVRDFICHIRRSDGQIR 647
Query: 628 TVEVSIEPITLNETACLLMTARDVS 652
E+S P+ + + C+L ARD++
Sbjct: 648 LCELSSRPLPIGDDDCMLTIARDIT 672
Score = 101 bits (251), Expect = 4e-25
Identities = 90/368 (24%), Positives = 173/368 (47%), Gaps = 43/368 (11%)
Query: 107 EQAMHHRIRWPDGSLHWLEINGSLIPDKQGRPRMIGVIREITHQRQREQALSSSEKRFAT 166
+ ++ +R+ DG W+ SLI + P + G++ +I+ ++ E+AL SE++FA+
Sbjct: 383 DHSIDYRVITADGRCLWVRDIVSLI-EHGHEPVLRGLMIDISEAKRTEEALQLSEQKFAS 441
Query: 167 LFHLCPNMVLLTRQEDGLISEANQYFESLFGWPVHSVIGRTTLELGLW----VNPEQRAK 222
+F CP+M+++ R DG + E N+ FE G V+G+T EL +W V P+ +
Sbjct: 442 VFQQCPDMLVIARLSDGCLLEVNKAFEDQIGLKAEHVVGKTATELNIWGIQGVGPDLLQR 501
Query: 223 LVKATKAKGELVSMEVQFRASNGQVHDGILSAQKVELEGQPYLLSTFLDTSERKLAEQAL 282
V+ T + ++E+ F SNGQ G++SA+ +L+ ++ D ++ K +Q L
Sbjct: 502 -VQTTSIR----NLEMPFLRSNGQAFTGLISAEPFQLDTIEAIVVVVRDITQLKETQQQL 556
Query: 283 KDSQERLDLALDSAQLGTWDWHIPSGMLYGSARAAQL----HGLDPIPFHEPFDAFFEGV 338
+ S+E+ A ++ P G+L R L G I + +
Sbjct: 557 QTSEEKFAKAFHAS---------PDGLLLSRQRDGLLIEVNDGFSRITGFTSATSLDQST 607
Query: 339 PD-------DERDSMRDAYRSLREGPAGNYQLTYRVQLPDGSSRYLESRARLYRDESGVP 391
D +ER M + + R+G ++ ++ DG R E +R +P
Sbjct: 608 LDLGIWVDLNERKHMLELMQ--RDGFVRDF--ICHIRRSDGQIRLCELSSR------PLP 657
Query: 392 LRMAGTLLDITDQVEREQRLITSEEKFASLFQVSPDPICVTRQDTGQFIE-INPAFTQIF 450
+ +L I + Q + ++ A++F+ + + + +T DT Q I +N AF++I
Sbjct: 658 IGDDDCMLTIARDITERQLMQEKLQQAATVFESTAEGVLIT--DTRQNISAVNRAFSEIT 715
Query: 451 GWTTTDVI 458
G++ + +
Sbjct: 716 GYSEAEAL 723
Score = 85.9 bits (211), Expect = 2e-20
Identities = 49/152 (32%), Positives = 78/152 (51%), Gaps = 1/152 (0%)
Query: 511 ISVGNEPCIVTTLRDITQQQRSEAALKASEEKFAKAFHSSPDAITITERDSGRYLEVNDG 570
I G+EP + + DI++ +R+E AL+ SE+KFA F PD + I G LEVN
Sbjct: 407 IEHGHEPVLRGLMIDISEAKRTEEALQLSEQKFASVFQQCPDMLVIARLSDGCLLEVNKA 466
Query: 571 FCRLTGYSADEVVGKTVYQIGIWAEEKQRSTLLAALKAKGRVHHQEMLGRNKRGEILTVE 630
F G A+ VVGKT ++ IW + LL ++ + + EM G+ T
Sbjct: 467 FEDQIGLKAEHVVGKTATELNIWGIQGVGPDLLQRVQTTS-IRNLEMPFLRSNGQAFTGL 525
Query: 631 VSIEPITLNETACLLMTARDVSLLKNAEAQIR 662
+S EP L+ +++ RD++ LK + Q++
Sbjct: 526 ISAEPFQLDTIEAIVVVVRDITQLKETQQQLQ 557
Score = 30.0 bits (66), Expect = 0.001
Identities = 40/154 (25%), Positives = 65/154 (42%), Gaps = 21/154 (13%)
Query: 146 EITHQRQREQALSSSEKRFATLFHLCPNMVLLTRQEDGLISEANQYFESLFGWP-VHSVI 204
EI + EQ L SSE + L + D S + + E L G+P H +I
Sbjct: 295 EIARRVALEQELRSSEYHYRGLVESLSAIAWEASVADFTYSYVSPHAEELLGYPRAHWLI 354
Query: 205 GRTTLELGLWVNPEQRAKLVKA-------TKAKGELVSMEVQFRASNGQ---VHDGILSA 254
G W N A L +A T+A + S++ + ++G+ V D +
Sbjct: 355 P------GFWRNIIHPADLTRAEAYCYRETRANRD-HSIDYRVITADGRCLWVRDIV--- 404
Query: 255 QKVELEGQPYLLSTFLDTSERKLAEQALKDSQER 288
+E +P L +D SE K E+AL+ S+++
Sbjct: 405 SLIEHGHEPVLRGLMIDISEAKRTEEALQLSEQK 438