Pairwise Alignments

Query, 785 a.a., NAD-dependent DNA ligase LigA from Pseudomonas simiae WCS417

Subject, 669 a.a., DNA ligase (NAD(+)) LigA from Vibrio cholerae E7946 ATCC 55056

 Score =  631 bits (1627), Expect = 0.0
 Identities = 340/600 (56%), Positives = 417/600 (69%), Gaps = 30/600 (5%)

Query: 1   MTAAHTRILELRAELDQHNYRYHVLDEPSIPDAEYDRLFHELKALEAEHPDLVTRDSPTQ 60
           M+    R+ ELR  L +H  RY+V D PSIPDAEYDRL  EL  LEA HP+L++ DSP+ 
Sbjct: 1   MSDVAQRLTELRKTLHEHGVRYYVEDAPSIPDAEYDRLMRELLELEAAHPELMSSDSPSL 60

Query: 61  RVGSAALSAFTQVKHEIPMLSLGNAFDETTMLEFDRRVTEGLDLPAGDLFGGGAAVEYS- 119
           RVG   L AF  V HEIPMLSL NAFD+  +  F RR+T+ +            AV++S 
Sbjct: 61  RVGGRPLDAFESVVHEIPMLSLDNAFDDGELESFYRRMTDRIP-----------AVQHSA 109

Query: 120 --CEPKLDGLAVSLLYQDGELVRGATRGDGTTGEDISVNVRTVRNIPLKLHGSGWPANLE 177
             CEPKLDGLAVSLLY++G L R ATRGDGTTGE+I+ NVRT+++IPL+L G+ +P  LE
Sbjct: 110 FCCEPKLDGLAVSLLYENGVLTRAATRGDGTTGENITENVRTIKSIPLRLQGADFPTRLE 169

Query: 178 VRGEVFMSKAGFERLNASQLEVGGKTFANPRNAAAGSLRQLDSKITASRPLEFCCYGVTT 237
           VRGEVFM KAGFE LNA  L+ G K F NPRNAAAGSLRQLDSKITA RPL F  Y V  
Sbjct: 170 VRGEVFMPKAGFEALNARALKKGEKQFVNPRNAAAGSLRQLDSKITAQRPLAFYAYSVGV 229

Query: 238 ----DISDTHIGNLQQLQKWGMPISRELKLAKGIQDCLEYYRDIGERRNSLPYEIDGVVF 293
               +++ +H     QL+ WG+PI  E KL   + +   +Y+DI +RR SL YEIDGVV 
Sbjct: 230 IEGGELATSHYQRFLQLKGWGLPICPETKLVTSLAEVKAFYQDILQRRQSLAYEIDGVVI 289

Query: 294 KVNSIASQRELGFRAREPRWAIAHKFPAMEELTELLDVEFQVGRTGAVTPVARLKPVKVA 353
           KV+ I  Q  LGF AR PRWAIA+KFPA EELT L DVEFQVGRTGA+TPVA+L+PV V 
Sbjct: 290 KVDDIQLQERLGFVARAPRWAIAYKFPAQEELTLLNDVEFQVGRTGAITPVAKLEPVFVG 349

Query: 354 GVTVSNATLHNMDEVARLGVMIGDTVIIRRAGDVIPQVVSVVLERRPENARAVPIPQSCP 413
           GVTVSNATLHN DE+ RLGVM+GDTV+IRRAGDVIPQ+VSVVLERRPENA+++  P  CP
Sbjct: 350 GVTVSNATLHNADEIERLGVMVGDTVVIRRAGDVIPQIVSVVLERRPENAKSIVFPTRCP 409

Query: 414 VCGSHVERTQLVKRSKGKETVSEGAVYRCVGRLACGAQLKQAIIHFVSRRAMDIDGLGDK 473
           VC S VER            V   AV RC G L C AQ K+A+ HFVSR+AMD++GLGDK
Sbjct: 410 VCQSDVER------------VEGEAVARCSGGLICQAQRKEALKHFVSRKAMDVEGLGDK 457

Query: 474 TIEQLVDEKLIGSPADLYKLKYEHIIDLEGFADISSKKLITAIENSKSPTLARFIYALGI 533
            IEQLVD +++ +PADL++L+   +  LE     S++ +I A+  +K  TL +F+YALGI
Sbjct: 458 VIEQLVDREMVSTPADLFRLRAGELTILERMGPKSAQNVIDALNKAKQTTLPKFLYALGI 517

Query: 534 PDVGEETAKVLARSLASLERVQKALPEVLTYLPDVGLEVAHEIHSFFEDSHNQDVIGALL 593
            +VGE TA  LA+   SLE +Q+A  E    +PDVG+ VA  + +FF    NQ VI  LL
Sbjct: 518 REVGEATALNLAQHFLSLEAIQQASLEQFIEVPDVGVVVASHLQAFFAQDRNQQVINELL 577



 Score = 89.0 bits (219), Expect = 8e-22
 Identities = 66/171 (38%), Positives = 90/171 (52%), Gaps = 10/171 (5%)

Query: 613 AASTTLGGLLDKLHVPSVGPGAAQKLADKFVTLEGVIKADWLDMRQALPEKQ---AKAVR 669
           A  TTL   L  L +  VG   A  LA  F++LE + +A  L+    +P+     A  ++
Sbjct: 503 AKQTTLPKFLYALGIREVGEATALNLAQHFLSLEAIQQAS-LEQFIEVPDVGVVVASHLQ 561

Query: 670 EFFDNADNAHRALAIEQQLKDFGMHWDS--EKKVVEGLPEAGHTWVLTGSLELMSRDVAK 727
            FF    N      +  +L + G+ W +     V      AG   VLTGS   +SR+ AK
Sbjct: 562 AFFAQDRNQQ----VINELLEQGITWPALTAAPVAVDSALAGKIVVLTGSFTQLSRNDAK 617

Query: 728 EKLESLGAKVAGSVSAKTHCVVAGPGAGSKLTKASELGLKVLDEEAFVAFL 778
             L++LGAKV GSVS  T  V AG  AGSKL KA+ELG++V DE+A + FL
Sbjct: 618 AALQALGAKVTGSVSKNTDMVFAGEAAGSKLAKATELGIQVFDEQALIEFL 668