Pairwise Alignments

Query, 374 a.a., tRNA 2-thiouridylase from Pseudomonas simiae WCS417

Subject, 372 a.a., tRNA 2-thiouridine(34) synthase MnmA from Vibrio cholerae E7946 ATCC 55056

 Score =  506 bits (1303), Expect = e-148
 Identities = 248/363 (68%), Positives = 297/363 (81%), Gaps = 4/363 (1%)

Query: 7   SDTQKKRVIVGMSGGVDSSVSAVLLMEQGYEVEGLFMKNWEEDDGTEYCTAMDDLADAQA 66
           S   +K+VIVGMSGGVDSSVSA LL +QGY+VEGLFMKNWEEDD  EYCTA +DLADAQA
Sbjct: 7   SANSQKKVIVGMSGGVDSSVSAYLLKQQGYQVEGLFMKNWEEDDNEEYCTAAEDLADAQA 66

Query: 67  VCDKIGIKLHTANFAAEYWDNVFEHFLAEYKAGRTPNPDILCNREIKFKAFLDYA-MMLG 125
           VCDK+GI LHT NFAAEYWDNVFE+FLAEYKAGRTPNPDILCN+EIKFKAFL++A  +L 
Sbjct: 67  VCDKLGIPLHTINFAAEYWDNVFEYFLAEYKAGRTPNPDILCNKEIKFKAFLEFADEVLD 126

Query: 126 ADLIATGHYVRRRDIDG--RTELLKGLDPNKDQSYFLHAVGGEQIAKTLFPVGELEKPEV 183
           AD IA GHYVRR       + ++L+GLD NKDQSYFL+ +  EQ+A+TLFPVGELEKPEV
Sbjct: 127 ADYIAMGHYVRRTFPQNGEKPQMLRGLDGNKDQSYFLYTLSHEQVARTLFPVGELEKPEV 186

Query: 184 RRIAEKHGLATAKKKDSTGICFIGERRFSDFLKQYLPAQPGEIKTTEGEVIGRHHGLMYH 243
           RRIAE+ GL TAKKKDSTGICFIGER+F+DFL +YLPAQPG+I+T EG+VIG H GLMYH
Sbjct: 187 RRIAEEQGLITAKKKDSTGICFIGERKFTDFLSRYLPAQPGKIETPEGKVIGEHQGLMYH 246

Query: 244 TIGQRQGLGIGGLKDAGEEPWYVLIKDLEHNELIVGQGNEHPLLFSGALLASEIYWVNPI 303
           T+GQR+GL IGG+K++ E+PWYV  KDL+ N LI  QG +HPLL S  L+A++++WV+  
Sbjct: 247 TLGQRKGLHIGGMKESSEQPWYVADKDLKRNVLIAVQGADHPLLKSHGLVAAQLHWVDRT 306

Query: 304 DLSTPRRLTAKVRYRQSDQPCTLEKTATG-YRAIFDDPQRAVTPGQSVVFYDGEICLGGG 362
            ++ P R + K RYRQSD  CT+   +    + +FD+PQ AVTPGQS VFY GEICLGGG
Sbjct: 307 PITEPVRCSVKTRYRQSDIACTIIPLSDDRIKVMFDEPQVAVTPGQSAVFYQGEICLGGG 366

Query: 363 VIE 365
           +IE
Sbjct: 367 IIE 369