Pairwise Alignments

Query, 626 a.a., DNA topoisomerase IV subunit B from Vibrio cholerae E7946 ATCC 55056

Subject, 804 a.a., DNA topoisomerase (ATP-hydrolyzing) subunit B GyrB from Escherichia coli ECRC62

 Score =  380 bits (977), Expect = e-110
 Identities = 226/555 (40%), Positives = 327/555 (58%), Gaps = 18/555 (3%)

Query: 1   MTEQYNAGAIEVLNGLEPVRRRPGMYT----DTTRPNHLGQEVIDNSVDEALAGYASKIQ 56
           M+  Y++ +I+VL GL+ VR+RPGMY     D T  +H+  EV+DN++DEALAG+  +I 
Sbjct: 1   MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNAIDEALAGHCKEII 60

Query: 57  VILHADQSLEVIDDGRGMPVDIHPEEKVSGVELILCKLHAGGKFSNKNYQFSGGLHGVGI 116
           V +HAD S+ V DDGRG+P  IHPEE VS  E+I+  LHAGGKF + +Y+ SGGLHGVG+
Sbjct: 61  VTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGGKFDDNSYKVSGGLHGVGV 120

Query: 117 SVVNALSKRVEVAVRRDGQVYEIAFEHGDKVTELTVTGTCGRRNTGTSVHFWPDTKYFDS 176
           SVVNALS+++E+ ++R+G+++   +EHG     L VTG      TGT V FWP  + F +
Sbjct: 121 SVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGE--TEKTGTMVRFWPSLETFTN 178

Query: 177 -PNFSVSRLINNLRAKAVLCPGLEIIFTDKVNNNEHRWLYEDGLKDYLAEGVKGYTLLPE 235
              F    L   LR  + L  G+ I   DK +  E  + YE G+K ++    K  T  P 
Sbjct: 179 VTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHYEGGIKAFVEYLNKNKT--PI 236

Query: 236 EPFTGEFTAQTE--AATWAVIWLPEGGELITESYVNLIPTAQGGTHVNGLRQGLLDAMRE 293
            P    F+ + +      A+ W  +G +     + N IP   GGTH+ G R  +   +  
Sbjct: 237 HPNIFYFSTEKDGIGVEVALQW-NDGFQENIYCFTNNIPQRDGGTHLAGFRAAMTRTLNA 295

Query: 294 FCEFRNLLPRG-VKLTGEDVFDRCAYVLSIKMQDPQFAGQTKERLSSRQSAAFVSGVVKD 352
           + +      +  V  TG+D  +    V+S+K+ DP+F+ QTK++L S +  + V   + +
Sbjct: 296 YMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSSQTKDKLVSSEVKSAVEQQMNE 355

Query: 353 AFSLWLNEKPQLAEQLAEVCI--ANAHSRMRASKKVVRKKIASGPA-LPGKLTDCTVQDL 409
             + +L E P  A+ +    I  A A    R ++++ R+K A   A LPGKL DC  +D 
Sbjct: 356 LLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMTRRKGALDLAGLPGKLADCQERDP 415

Query: 410 NRTELFLVEGDSAGGSAKQARDREFQAVMPLRGKILNTWEVSADQVLASQEVHDISVAL- 468
             +EL+LVEGDSAGGSAKQ R+R+ QA++PL+GKILN  +   D++L+SQEV  +  AL 
Sbjct: 416 ALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKILNVEKARFDKMLSSQEVATLITALG 475

Query: 469 -GIDPDSDNLEALRYGKVCILADADSDGLHIATLLCALFTRHFRALVKAGHVYVAMPPLY 527
            GI  D  N + LRY  + I+ DAD DG HI TLL   F R    +V+ GHVY+A PPLY
Sbjct: 476 CGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRTLLLTFFYRQMPEIVERGHVYIAQPPLY 535

Query: 528 RIDCGKEVFYALDDQ 542
           ++  GK+  Y  DD+
Sbjct: 536 KVKKGKQEQYIKDDE 550



 Score = 68.6 bits (166), Expect = 1e-15
 Identities = 31/70 (44%), Positives = 51/70 (72%), Gaps = 1/70 (1%)

Query: 551 LSQKKAKVNVQRFKGLGEMNPLQLRETTMDPNTRRLVQLTIDDAEATDEMMDMLLGKKRA 610
           + + +  +++QR+KGLGEMNP QL ETTMDP +RR++++T+ DA A D++   L+G    
Sbjct: 727 VKESRRGLSIQRYKGLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMG-DAV 785

Query: 611 DDRRAWLQRN 620
           + RRA+++ N
Sbjct: 786 EPRRAFIEEN 795