Pairwise Alignments
Query, 2413 a.a., non-ribosomal peptide synthetase from Vibrio cholerae E7946 ATCC 55056
Subject, 1818 a.a., pyochelin synthetase from Pseudomonas simiae WCS417
Score = 295 bits (755), Expect = 5e-83
Identities = 250/903 (27%), Positives = 408/903 (45%), Gaps = 61/903 (6%)
Query: 457 PLTPLQQAYLLGRSTQIALGGVAMHEFREYRG-HIDTQSLHSRLLYLVEHIPALRTRIDQ 515
PLT +Q AYLLGR + G VA H + E +D Q + L+ LR I
Sbjct: 72 PLTDVQNAYLLGRQSSFGYGNVACHGYLELSWPSLDPQQVELAWNRLIARHDMLRAVIAS 131
Query: 516 EKWIQWVSPCIALNWQAIDLQHLSREQALLAVEPVRQQYQQRMHDLTRSPWQICVVQLPI 575
E + ++ + DL+ +R Q V+ +RQ +Q+++ W + ++L
Sbjct: 132 EGSQRVLADVAHYSLTIADLRGATRAQHQHTVQQIRQAMEQKLYPT--EVWPLFELRL-- 187
Query: 576 EEQEEFSSIVLTSFDALIVDGRTHALI---LAALLGSEEPDITQVVQNARDTQSISPQF- 631
+ + + S D+LI D + L+ L LL + + + + RD
Sbjct: 188 -SRSDNGDTLHFSMDSLIADWASAQLLFNELELLLQNPQAALPALDITFRDYLLAERSLL 246
Query: 632 -ASKKAQDEAYWKSKLHPDCPPPALPWKQALETITTSRYARESLQIPKESVGKLNRCGIE 690
S+ +D YW +++ P LP + E R+ R S ++ + L E
Sbjct: 247 EGSRHQRDRDYWLTRIDALPAAPQLPTPLSGEDTREPRFQRHSARLEPQPWAALKSAASE 306
Query: 691 NGLFLNSLLTATILDVLSYWTTEIGMRVGFPVLI-----PSSNAIDGNESSFVILE-HEK 744
L + ++ A L W+ + + +L P + + G+ +S +L+ ++
Sbjct: 307 RSLTPSIVVLAAYAATLQRWSQQPAFSLNLTLLNRLPLHPQVDRLVGDFTSVSLLQVNDP 366
Query: 745 STLSLLSQASKLQREMLEALEHLAFSGVDLNRLL-MNQAPQALVLPVVLTNGLSWKT-LN 802
L +QA +L ++ LEH FSG+ + R + + +A +PVV T+ +
Sbjct: 367 EGLDFTAQARQLGNQLFTDLEHRLFSGIQVMREIGRRRGREAAAMPVVFTSAIGLGIEAP 426
Query: 803 PEDAVTLFDGVTQTPQVALDIRLTYDEQKNLIISFDYALAVLETELIREMLSALHHRLSQ 862
P + G+TQTPQV LD ++ D+ L I +D V L+ +M A L
Sbjct: 427 PASQRRVGHGITQTPQVTLDCQVM-DDANGLHIHWDVRQGVFPDGLVDDMQQAFVAELQL 485
Query: 863 IT-SSASLAAPLEPCIDLSHYRFNSDESASHDCDFLAKLAQQLFVRTEDKT---AVICGE 918
+ A+ P++ + R +A+ +L L + AVI
Sbjct: 486 LALDPAAWEHPVQVPLPAWQQRERLAANATAAPLPTGRLHDGLLAMAWEHPHALAVIDAS 545
Query: 919 QTLSYAQLGEQVQRVMWQLKARGLTTGNVLAICLPRSVEHIVISLASALSGIIWVPIDAA 978
+L+YA L E+ V L+ G +AI +P+ ++ +V + L+G ++P+D+
Sbjct: 546 GSLTYAALAERALAVASALREAGCDAQEPVAILMPKGLDQVVAAYGVLLAGAAYLPLDSN 605
Query: 979 SPKERLNYLLENCHADLVV----------MDKPCEFGNVIAFDALIEPVLFADGVPDVTP 1028
+P R + +L + V+ + + ++ +V + +G PD
Sbjct: 606 APAARRDRVLRSAKVRHVLGHSLAQPHTPLPETSQWHSVDRLPSAGADFELLEGSPD--- 662
Query: 1029 LDQLSRLSQSQQTAYYLYTSGTTGKPKCVVVNNQATSNVIGQTGQAWHLTSEDVVMSVTP 1088
AY +YTSG+TG+PK V+++++A N + + + + ++D V+ +
Sbjct: 663 -----------HLAYVIYTSGSTGEPKGVMISHRAALNTVQDINRRFEVNADDRVLGLAQ 711
Query: 1089 LHHDMSVFDLFATLSFGATLVLPAGHEEKDALQWNRLIERHQVTIWVSVPAILEMLLSCT 1148
L D+SV+DLF L+ G TLVLP D W L++RHQVT+W SVPA L ML
Sbjct: 712 LSFDLSVYDLFGPLAVGGTLVLPDPARGADPSHWAELVQRHQVTLWNSVPAQLHMLADYL 771
Query: 1149 QA--GQLHSLRLIAQGGDYIKPATIAQLRAGSNPPRLISLGGPTETTIWSIWHELTADDV 1206
A L SLRL GD+I QL+A +L+ LGG TE +IWS H + A D
Sbjct: 772 HAEPRPLDSLRLALLSGDWIPLNLPPQLKALLPELKLVGLGGATEASIWSNLHPIGAIDP 831
Query: 1207 S--VIPYGRPLAGNRYFIMDEIQRHVPQGVVGRIFTSGVNLAQGYLEDGELKQTDFVTVL 1264
+ IPYG PLA + ++D R P V G ++ +GV LAQGYL D +L F
Sbjct: 832 AWRSIPYGLPLANQGFRVLDHHWRDAPTWVPGDLYITGVGLAQGYLGDMDLSDARFFP-- 889
Query: 1265 DEHGHPV---RAFRTGDQGYYRADGNIIFASRINGYVKVRGVRVSLPDIEKQLQTHPALA 1321
HP+ R +RTGD+G Y G + F R +G VK+RG RV L ++E L P +
Sbjct: 890 ----HPLDGQRLYRTGDRGRYLPGGELEFLGREDGQVKIRGHRVELGEVEAALLATPGVD 945
Query: 1322 SVV 1324
S V
Sbjct: 946 SAV 948
Score = 150 bits (380), Expect = 1e-39
Identities = 123/439 (28%), Positives = 201/439 (45%), Gaps = 16/439 (3%)
Query: 4 MTAMQAAYWLGRQHDCLLDGVAAHLYAEFDGQALNRQALTEAVRALYAKHPMLRLAITKD 63
+T +Q AY LGRQ VA H Y E +L+ Q + A L A+H MLR I +
Sbjct: 73 LTDVQNAYLLGRQSSFGYGNVACHGYLELSWPSLDPQQVELAWNRLIARHDMLRAVIASE 132
Query: 64 GQQKILPLSTFHQLKVDDLSQWKPDEVESFVHTKRQRMTHQMLDLTQGNPIEISLTLLPE 123
G Q++L + L + DL + + V RQ M ++ E+ L+
Sbjct: 133 GSQRVLADVAHYSLTIADLRGATRAQHQHTVQQIRQAMEQKLYPTEVWPLFELRLSRSDN 192
Query: 124 GKHRLHIDADMIACDAQSFRLLVDDLTSLYLEAIEHRLEIIESDVVTFFQYLDAQQADRA 183
G LH D + D S +LL ++L L L+ + L ++ +TF YL A+R+
Sbjct: 193 GD-TLHFSMDSLIADWASAQLLFNEL-ELLLQNPQAALPALD---ITFRDYL---LAERS 244
Query: 184 LAK--RKEVDKKWWQERLATIPAEPSLPYQPVPTDAVSANSQRFAHWFTPVERKGLAEVA 241
L + R + D+ +W R+ +PA P LP D QR + P L A
Sbjct: 245 LLEGSRHQRDRDYWLTRIDALPAAPQLPTPLSGEDTREPRFQRHSARLEPQPWAALKSAA 304
Query: 242 RQHHLTLTQLTLALFSQVIANACQERQFRLNVPTFHRGNRSLDIEHTIGDFSNLLIFSAD 301
+ LT + + LA ++ + Q+ F LN+ +R ++ +GDF+++ + +
Sbjct: 305 SERSLTPSIVVLAAYAATLQRWSQQPAFSLNLTLLNRLPLHPQVDRLVGDFTSVSLLQVN 364
Query: 302 VGTTQTLLSLCQQTANQLHQLLRHESYSGVSVMRDLSRKQG-GVQRSPIVFTSGMEMRDE 360
+ +Q NQL L H +SG+ VMR++ R++G P+VFTS + + E
Sbjct: 365 DPEGLDFTAQARQLGNQLFTDLEHRLFSGIQVMREIGRRRGREAAAMPVVFTSAIGLGIE 424
Query: 361 EIFSDHVTQHLGRMNWVISQGAQVTLDAQIAPAYEGILLNWDVRMENFADKDITALFAHY 420
+ +Q R+ I+Q QVTLD Q+ G+ ++WDVR F D + + +
Sbjct: 425 ---APPASQR--RVGHGITQTPQVTLDCQVMDDANGLHIHWDVRQGVFPDGLVDDMQQAF 479
Query: 421 VDLIRCVALHPEMMQQSVQ 439
V ++ +AL P + VQ
Sbjct: 480 VAELQLLALDPAAWEHPVQ 498
Score = 39.7 bits (91), Expect = 5e-06
Identities = 20/42 (47%), Positives = 25/42 (59%)
Query: 1347 QSASSQALREFAKQSLPSSHIPSRFIALEALPLSANGKVDRK 1388
Q S +LR Q LP +P+ L+ LPL+ANGKVDRK
Sbjct: 1344 QRLSVDSLRRALAQRLPDYMLPAHLQVLDRLPLTANGKVDRK 1385
Score = 34.7 bits (78), Expect = 2e-04
Identities = 24/84 (28%), Positives = 39/84 (46%), Gaps = 6/84 (7%)
Query: 1872 ALGVAEMTAEDDFFDFGGHSLIATRVIGRLLSE------QGIELHINDMFSFPNAKQLAQ 1925
ALG+A++ ED F++ G SLI RV G+L + + + M + PN LA+
Sbjct: 1420 ALGLAQIGVEDSFYEKGADSLILARVAGQLREQLPQAQSLSYDTLLRQMLNEPNVAALAR 1479
Query: 1926 QAVLHRKPTSTSFAVSEVVESSKA 1949
+ + A E SS++
Sbjct: 1480 LLNQGKTTPARVAASGEAQRSSRS 1503
Score = 31.2 bits (69), Expect = 0.002
Identities = 64/278 (23%), Positives = 106/278 (38%), Gaps = 34/278 (12%)
Query: 1900 RLLSEQ---GIELHIND---MFSFP----NAKQLAQ-----QAVLHRKPTSTSFAVSEVV 1944
+LLSE G+EL D F P + ++LAQ Q +LH T V
Sbjct: 5 KLLSELKSLGVELWPQDGQLKFRAPQGLLDTERLAQLREHKQQILHLLETQERPLVLADP 64
Query: 1945 ESSKAPLSLAQASLWKAMSKYAKFGLTHIFNLPFALKFLDEVNEQAFGEAFHWLLLRHAG 2004
P L + + + FG ++ + ++ Q A++ L+ RH
Sbjct: 65 AQRHTPFPLTDVQNAYLLGRQSSFGYGNVACHGYLELSWPSLDPQQVELAWNRLIARHDM 124
Query: 2005 LRTHFGLEDGQPYQHVIAASNIEHYQWFWTSKDNAA----QSVARLLAQEAEHTFDLSQE 2060
LR E Q V+A ++ HY A Q + + Q E ++
Sbjct: 125 LRAVIASEGSQ---RVLA--DVAHYSLTIADLRGATRAQHQHTVQQIRQAMEQKLYPTEV 179
Query: 2061 LPL-RLNFVRDEQTGTQYLSLLFHHIVLDEWSINILMDELAQVYQHSVQGTRPQWQTEPL 2119
PL L R + T + S+ ++ D S +L +EL + Q+ Q P +
Sbjct: 180 WPLFELRLSRSDNGDTLHFSM--DSLIADWASAQLLFNELELLLQNP-QAALPALD---I 233
Query: 2120 PFHEFARKQRS---SAFNQTHLNYWLTKFAGVPWAQPL 2154
F ++ +RS + +Q +YWLT+ +P A L
Sbjct: 234 TFRDYLLAERSLLEGSRHQRDRDYWLTRIDALPAAPQL 271