Pairwise Alignments

Query, 951 a.a., formate dehydrogenase from Vibrio cholerae E7946 ATCC 55056

Subject, 1016 a.a., formate dehydrogenase-O, large subunit (NCBI) from Escherichia coli BW25113

 Score =  243 bits (620), Expect = 5e-68
 Identities = 188/642 (29%), Positives = 286/642 (44%), Gaps = 124/642 (19%)

Query: 19  ISRRAFMKNTSLAAGGAAVGASLFTPGMIRKAQASEVDRSAKTEVKRTICSHCSVGCGIY 78
           +SRR F K  +    G    A  F P  +  A+  +  +  +T   R  C++CSVGCG+ 
Sbjct: 3   VSRRQFFKICAGGMAGTTAAALGFAPS-VALAETRQY-KLLRTRETRNTCTYCSVGCGLL 60

Query: 79  A-EVQNGVWTGQEPAF------DHPFNAGGHCAKGAALREHGHGERRLKYPMKLEGG--K 129
              + +G    +   F      DHP N G  C KGA L +  H E RLK+P     G  K
Sbjct: 61  MYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFIHSESRLKFPEYRAPGSDK 120

Query: 130 WKKISWEQAINEIGDKALKIREESGPD------------SVYFLGSAKHSNEQAYLFRKM 177
           W++ISWE+A + I     + R+ +               S   L ++  SNE  YL +K 
Sbjct: 121 WQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLSTGMLCASASSNETGYLTQKF 180

Query: 178 ASLWGTNNVDHQARICHSTTVAGVANTWGYGAMTNSFNDMHNCKSMLFIGSNPAEAHPVA 237
           +   G   VD+QAR+ H  TVA +A T+G GAMTN + D+ N   ++ +G N AEAHPV 
Sbjct: 181 SRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWVDIKNANLVVVMGGNAAEAHPVG 240

Query: 238 MQHILIAKEKNSCKIVVADPRRTRTAAKADYFVSLRPGSDVAFIWGVLWHVFKNNWEDKE 297
            +  + AK  N  K++V DPR TRTAA ADY+  +R G+D+AF+ GVL ++  N   ++E
Sbjct: 241 FRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIRSGTDIAFLSGVLLYLLNNEKFNRE 300

Query: 298 Y---------IRQRVFGMDE---------------------------------------- 308
           Y         I +  +G ++                                        
Sbjct: 301 YTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYDKSSWTYELDENGFAKRDTTLQHPRCV 360

Query: 309 ---IRAEVAKWTPAEVERVTGVSEEEVYNT----AKTLAENRPGCVVWCMGGTQHTTGNN 361
              ++  V+++TP  VE + G  ++         A+T A ++    ++ +G TQH+ G  
Sbjct: 361 WNLLKQHVSRYTPDVVENICGTPKDAFLKVCEYIAETSAHDKTASFLYALGWTQHSVGAQ 420

Query: 362 NTRAYCILELALGNIGKSGGGANIFRGHDNVQGATDLGVLSDTLPGYY------------ 409
           N R   +++L LGN+G +GGG N  RGH N+QG TDLG+LS +LPGY             
Sbjct: 421 NIRTMAMIQLLLGNMGMAGGGVNALRGHSNIQGLTDLGLLSQSLPGYMTLPSEKQTDLQT 480

Query: 410 --------GLTEGSWKHWASVWGVDFEWIKNRF-DQGTYNGA-----LPMETPGIPVSRW 455
                    L EG   +W +        +K  F D+ T   +     LP    G  V ++
Sbjct: 481 YLTANTPKPLLEGQVNYWGNYPKFFVSMMKAFFGDKATAENSWGFDWLPKWDKGYDVLQY 540

Query: 456 IDGVLENKDNLQQRENIRAMFYWGHAVNSQTRGVEMKKAMQKLDMMVIVDPYPTVAA--- 512
            + + E K N    +         + V S     ++   + KL  +V +DP  T  +   
Sbjct: 541 FEMMKEGKVNGYICQGF-------NPVASFPNKNKVIGCLSKLKFLVTIDPLNTETSNFW 593

Query: 513 ----VMNDRTDG-----VYLLPATTQFETYGSVTASNRSIQW 545
                +N+         V+ LP+T   E  GS+  S R +QW
Sbjct: 594 QNHGELNEVDSSKIQTEVFRLPSTCFAEENGSIVNSGRWLQW 635



 Score = 49.7 bits (117), Expect = 1e-09
 Identities = 58/190 (30%), Positives = 83/190 (43%), Gaps = 31/190 (16%)

Query: 770  PLHREPLYTPR-RDLLADYPTWDDQAFIFRVPTLYKSIQAQDKSVEYPIILTSGRLVEYE 828
            P H EP  TP   + L      +  A IF+        +A  K+ ++P + T+ RL E+ 
Sbjct: 848  PEHYEPFETPLGTNPLHPNVISNPAARIFK-----DDAEALGKADKFPYVGTTYRLTEHF 902

Query: 829  GGGEETRSNPWLAELQQEMFVEVNPKDANDLGFMDGDMVWVEGAEKGRIKVKAMVTRRVK 888
                 T+     A LQ E FVE+    AN LG   GD V V  + +G IK KA+VT+R++
Sbjct: 903  HYW--TKHALLNAILQPEQFVEIGESLANKLGIAQGDTVKV-SSNRGYIKAKAVVTKRIR 959

Query: 889  PGMA--------FLPFHFGGKFQGEDLRPKYPEGTQPYVVGEAANTATTYGYDPVTLMQE 940
               A         +P H+G  ++G   +            G  ANT T +  D  T   E
Sbjct: 960  TLKANGKDIDTIGIPIHWG--YEGVAKK------------GFIANTLTPFVGDANTQTPE 1005

Query: 941  TKVTLCNIRK 950
             K  L N+ K
Sbjct: 1006 FKSFLVNVEK 1015