Pairwise Alignments

Query, 879 a.a., MCE family protein from Vibrio cholerae E7946 ATCC 55056

Subject, 546 a.a., intermembrane transport protein PqiB from Escherichia coli ECRC62

 Score =  166 bits (419), Expect = 5e-45
 Identities = 94/310 (30%), Positives = 158/310 (50%), Gaps = 10/310 (3%)

Query: 14  EIRKRRGISPLWILPIVTMILAGWLVFKAVHDAGVRIQIHFENAQGLIAGRTTIRYQGLE 73
           +I+K +  SP+WI PIVT ++  W++F      G  + +   NA+G+  G+TTI+ + ++
Sbjct: 9   KIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPEVTLITANAEGIEGGKTTIKSRSVD 68

Query: 74  VGMVRDIKLSEGLDSIYVEADIYPEATKLLSNQTRFWMVKPTASLSGVSGLDALVSGNYI 133
           VG+V    L++ L  + ++A +     KLL   T FW+VKP     G+SGL  L+SG YI
Sbjct: 69  VGVVESATLADDLTHVEIKARLNSGMEKLLHKDTVFWVVKPQIGREGISGLGTLLSGVYI 128

Query: 134 AIQPGSTHQEDYPTQYQALDSAPSDLLAQRGLTISLKARDLGGISVGSQIVYKKIPIGEV 193
            +QPG+   +    +Y  LDS P      +G+ + L ++  G +S G  ++++   +G V
Sbjct: 129 ELQPGAKGSK--MDKYDLLDSPPLAPPDAKGIRVILDSKKAGQLSPGDPVLFRGYRVGSV 186

Query: 194 FSYQLDDDAQSVIIQASIKEEYQHIINTESRFWNVSGIGASIGFEGVDVRLESLSALIGG 253
            +   D   +++  Q  I   Y  ++    RFW  SGI   +   G+ V + SL+ L+ G
Sbjct: 187 ETSTFDTQKRNISYQLFINAPYDRLVTNNVRFWKDSGIAVDLTSAGMRVEMGSLTTLLSG 246

Query: 254 SIAVDSP---DEGKPVEQNAQFRLYRDLKTAGRGIAVS-----ITLPDDNNISASGAPIM 305
            ++ D P   D G+PV     F LY D K+    +        +   D       GAP+ 
Sbjct: 247 GVSFDVPEGLDLGQPVAPKTAFVLYDDQKSIQDSLYTDHIDYLMFFKDSVRGLQPGAPVE 306

Query: 306 YRGIEIGQIT 315
           +RGI +G ++
Sbjct: 307 FRGIRLGTVS 316



 Score = 87.8 bits (216), Expect = 2e-21
 Identities = 71/245 (28%), Positives = 113/245 (46%), Gaps = 12/245 (4%)

Query: 625 WKLYSDYDHARRYGEKITLTALGTLGVKVG-TPVQYQGVQIGEVFEIIPDFESDFVKLAA 683
           W L+  Y H    G ++TL      G++ G T ++ + V +G V       +   V++ A
Sbjct: 32  WVLFYHYSHQ---GPEVTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKA 88

Query: 684 RIEPQYAPKIAKQNSQFWLSQAKIGLSGIENVQNLL-GQSIEVQPG-NGESRFEFELHKE 741
           R+      K+  +++ FW+ + +IG  GI  +  LL G  IE+QPG  G    +++L   
Sbjct: 89  RLNSGME-KLLHKDTVFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDS 147

Query: 742 ---ARHGGAGNTYTLQSEKRGSVSVGTPILYRDIEVGKVIDVRLGEFADRVITTIRIAPQ 798
              A     G    L S+K G +S G P+L+R   VG V           +   + I   
Sbjct: 148 PPLAPPDAKGIRVILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAP 207

Query: 799 YTYLLRQNSVFWNVSGLDMSIGITGANVKAGTFDSMLRGGITFATPEQKQL-TPAAPEGH 857
           Y  L+  N  FW  SG+ + +   G  V+ G+  ++L GG++F  PE   L  P AP+  
Sbjct: 208 YDRLVTNNVRFWKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPK-T 266

Query: 858 TFYLY 862
            F LY
Sbjct: 267 AFVLY 271



 Score = 78.6 bits (192), Expect = 1e-18
 Identities = 78/316 (24%), Positives = 138/316 (43%), Gaps = 48/316 (15%)

Query: 403 LVADNSFGLEAG-TPILYRGVAVGSVTAVNLK--LDYVEFNVLIDEQYGALIRSQNRFYV 459
           L+  N+ G+E G T I  R V VG V +  L   L +VE    ++     L+     F+V
Sbjct: 47  LITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDTVFWV 106

Query: 460 TGSAAAELTESGLSVSIPPAKQLLLGS-ISFASEGSSTPLEQYRLYSSQSLAELAKYNQS 518
                     SGL         LL G  I        + +++Y L  S  LA     +  
Sbjct: 107 VKPQIGREGISGLGT-------LLSGVYIELQPGAKGSKMDKYDLLDSPPLAPP---DAK 156

Query: 519 GSRSLTLFAHELPSINAGSPLLYRNLKVGSI--SGFTLTPKGVQIEATIEKQYQHLLTPD 576
           G R + L + +   ++ G P+L+R  +VGS+  S F    + +  +  I   Y  L+T +
Sbjct: 157 GIRVI-LDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTNN 215

Query: 577 TVFWNRSGVEIKASMDGVDVKAAPLQTLIRGGIAFDNLPGI---------------ENKV 621
             FW  SG+ +  +  G+ V+   L TL+ GG++FD   G+               +++ 
Sbjct: 216 VRFWKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQK 275

Query: 622 GSMWKLYSDY-DHARRYGEKITLTALGTLGVKVGTPVQYQGVQIGEVFEI---IPDFESD 677
                LY+D+ D+   + + +        G++ G PV+++G+++G V ++    P+    
Sbjct: 276 SIQDSLYTDHIDYLMFFKDSVR-------GLQPGAPVEFRGIRLGTVSKVPFFAPNMRQT 328

Query: 678 F-----VKLAARIEPQ 688
           F     + +  RIEP+
Sbjct: 329 FNDDYRIPVLIRIEPE 344



 Score = 49.3 bits (116), Expect = 6e-10
 Identities = 61/297 (20%), Positives = 120/297 (40%), Gaps = 44/297 (14%)

Query: 161 AQRGLTISLKARDLGGISVG-SQIVYKKIPIGEVFSYQLDDDAQSVIIQASIKEEYQHII 219
           + +G  ++L   +  GI  G + I  + + +G V S  L DD   V I+A +    + ++
Sbjct: 39  SHQGPEVTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLL 98

Query: 220 NTESRFW---------NVSGIGASIGFEGVDVRLESLSALIGGSIAVDSPDEGKPVEQNA 270
           + ++ FW          +SG+G  +    ++++  +  + +     +DSP    P  +  
Sbjct: 99  HKDTVFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKG- 157

Query: 271 QFRLYRDLKTAGRGIAVSITLPDDNNISASGAPIMYRGIEIGQITDLQLTENRKSIVASA 330
             R+  D K AG+               + G P+++RG  +G +        +++I    
Sbjct: 158 -IRVILDSKKAGQ--------------LSPGDPVLFRGYRVGSVETSTFDTQKRNISYQL 202

Query: 331 AIQPAFSDMLNQGSQFVLEEA-QVSLTG------VENLTNLVKGNYLTLIPGAGERTRNF 383
            I   +  ++    +F  +    V LT       + +LT L+ G     +P  G      
Sbjct: 203 FINAPYDRLVTNNVRFWKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVP-EGLDLGQP 261

Query: 384 QAVRKNEFKYARSNSISFNLVA----------DNSFGLEAGTPILYRGVAVGSVTAV 430
            A +     Y    SI  +L            D+  GL+ G P+ +RG+ +G+V+ V
Sbjct: 262 VAPKTAFVLYDDQKSIQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKV 318