Pairwise Alignments

Query, 310 a.a., tRNA 2-thiocytidine(32) synthetase TtcA from Vibrio cholerae E7946 ATCC 55056

Subject, 311 a.a., predicted C32 tRNA thiolase (NCBI) from Escherichia coli BW25113

 Score =  483 bits (1243), Expect = e-141
 Identities = 233/312 (74%), Positives = 270/312 (86%), Gaps = 9/312 (2%)

Query: 4   QTQELTKAQQYNFNKLQKRIRRNTGQAIADFNMIEDGDRIMVCLSGGKDSFTMLDILMSL 63
           + Q++TK +QYN NKLQKR+RRN G+AIADFNMIE+GDRIMVCLSGGKDS+TML+IL +L
Sbjct: 3   ENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMVCLSGGKDSYTMLEILRNL 62

Query: 64  QKSAPISFELVAVNLDQKQPGFPEHVLPEYLESLGVEYKIVEEDTYAIVQDKVPEGKTTC 123
           Q+SAPI+F LVAVNLDQKQPGFPEHVLPEYLE LGVEYKIVEE+TY IV++K+PEGKTTC
Sbjct: 63  QQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKIVEENTYGIVKEKIPEGKTTC 122

Query: 124 ALCSRLRRGILYRTAKELGATKIALGHHRDDILETLFLNMFYGGKMKGMPPKLVSDNGEH 183
           +LCSRLRRGILYRTA ELGATKIALGHHRDDIL+TLFLNMFYGGKMKGMPPKL+SD+G+H
Sbjct: 123 SLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFLNMFYGGKMKGMPPKLMSDDGKH 182

Query: 184 VVIRPLAYCREKDIIKYSDMRGYPIIPCNLCGSQPNLQRQAVKQMLNDWDKRFPGRIETM 243
           +VIRPLAYCREKDI +++D + +PIIPCNLCGSQPNLQRQ +  ML DWDKR+PGRIETM
Sbjct: 183 IVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPNLQRQVIADMLRDWDKRYPGRIETM 242

Query: 244 FRAMQNVVPSHLADFSLFDFKSIDKNSGVINGGDIGFDKEEIAPQVV-----EDEDLVME 298
           F AMQNVVPSHL D +LFDFK I   S V+NGGD+ FD+EEI  Q       EDE+ + E
Sbjct: 243 FSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGDLAFDREEIPLQPACWQPEEDENQLDE 302

Query: 299 FDPSLQLNVTNI 310
               L+LNV  +
Sbjct: 303 ----LRLNVVEV 310