Pairwise Alignments

Query, 591 a.a., aspartate--tRNA ligase from Vibrio cholerae E7946 ATCC 55056

Subject, 505 a.a., lysine tRNA synthetase, constitutive from Escherichia coli BL21

 Score = 85.1 bits (209), Expect = 6e-21
 Identities = 77/307 (25%), Positives = 148/307 (48%), Gaps = 27/307 (8%)

Query: 1   MRSHYCGHLNKSLVGQTVELC--GWVNRRRDLGGLIFIDMRDREGIVQVVVDPDMADVFA 58
           + + + G  N+ L    +E+   G +  RR +G   F+ ++D  G +Q+ V  D      
Sbjct: 49  LHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARDDLPEGV 108

Query: 59  VANQLRSEFCIKLTGEVRARPESQVNKEMATGEVELLARSLEIINRS-DVLPLDFNQKNS 117
              Q +      + G   A+ +    K   TGE+ +    L ++ ++   LP  F+    
Sbjct: 109 YNEQFKKWDLGDILG---AKGKLFKTK---TGELSIHCTELRLLTKALRPLPDKFHGLQD 162

Query: 118 EEQRLKYRYLDL-RRPEMSDRIKLRAKASSFVRRFLDTHGFLDIETPVLTKATPEGA--R 174
           +E R + RYLDL    E  +  K+R++  S +R+F+   GF+++ETP++ +  P GA  R
Sbjct: 163 QEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMM-QVIPGGAAAR 221

Query: 175 DYLVPSRVHKGSFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRDEDLRADRQPEFTQIDI 234
            ++          Y L  +P+L+ + L++ GF+R ++I + FR+E +     PEFT +++
Sbjct: 222 PFITHHNALDLDMY-LRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFTMMEL 280

Query: 235 ETSFMTAEQVRAVTEKMIREMWLELL---NVDLGD--------FPIMPYSEAMRRF--GS 281
             ++   + +  +TE + R +  ++L    V  GD        F  +   EA++++   +
Sbjct: 281 YMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIKKYRPET 340

Query: 282 DKPDLRN 288
           D  DL N
Sbjct: 341 DMADLDN 347



 Score = 63.5 bits (153), Expect = 2e-14
 Identities = 50/179 (27%), Positives = 82/179 (45%), Gaps = 16/179 (8%)

Query: 381 IERTQAQTGDIILFGADSAKVVAEALGALRLKAGKELGITNESAWAPLWVVDFPMFESDD 440
           I++ + +T    L   DSAK +AE++G           I  E +W  L  +   +FE   
Sbjct: 333 IKKYRPETDMADLDNFDSAKAIAESIG-----------IHVEKSWG-LGRIVTEIFEEVA 380

Query: 441 EGNVAAMHHPFTSPLNLSPEQLKANPEEALSNAYDMVLNGYEVGGGSVRIHNAEMQSAVF 500
           E ++         P  +SP   + +    +++ ++  + G E+G G   +++AE Q+  F
Sbjct: 381 EAHLIQPTFITEYPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRF 440

Query: 501 ----DILGITPEEQRLKFGFLLDALKFGTPPHAGLAFGLDRLVMLLCGTENIRDVIAFP 555
                      +E        + AL+ G PP AGL  G+DR+VML   +  IRDVI FP
Sbjct: 441 LDQVAAKDAGDDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFP 499