Pairwise Alignments

Query, 858 a.a., leucine--tRNA ligase from Vibrio cholerae E7946 ATCC 55056

Subject, 951 a.a., valyl-tRNA synthetase (NCBI) from Escherichia coli BW25113

 Score =  131 bits (330), Expect = 2e-34
 Identities = 199/909 (21%), Positives = 322/909 (35%), Gaps = 219/909 (24%)

Query: 1   MQEQYNPQDIEHKVQQHWDNNKTFVVSEDPNKEKFYCLSMFPYPSGRLHMGHVRNYTIGD 60
           M++ YNPQDIE  + +HW+    F  + D ++E F  +   P  +G LHMGH    TI D
Sbjct: 1   MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNVTGSLHMGHAFQQTIMD 60

Query: 61  VVSRFQRLQGKNVLQPIGWDAFGLPAENAAVKNNTAPA-----------------PWTYE 103
            + R+QR+QGKN L  +G D  G+  +    +   A                    W  E
Sbjct: 61  TMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTRHDYGREAFIDKIWEWKAE 120

Query: 104 NIEYMKNQLKLLGFGYDWNREFATCRPEYYRWEQEFFTKLFAKGLVYKKTSSVNWCPNDQ 163
           +   +  Q++ LG   DW RE  T         +E F +L+ + L+Y+    VNW P  +
Sbjct: 121 SGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRLYKEDLIYRGKRLVNWDPKLR 180

Query: 164 TVLANEQVEDGCCWRCDTPVEQKEIPQWFIKITAYAQELLDDLDNLDGWPEMVKTMQRNW 223
           T +++ +VE+           + +   W I+       L D     DG   +V       
Sbjct: 181 TAISDLEVEN----------RESKGSMWHIR-----YPLADGAKTADGKDYLV------- 218

Query: 224 IGRSEGVELKFAVKGENTDLEVYTTRPDTLMGVTYVGIAAGHPLATKAAANNPALAAFID 283
                                V TTRP+TL+G T V +             NP    + D
Sbjct: 219 ---------------------VATTRPETLLGDTGVAV-------------NPEDPRYKD 244

Query: 284 ECKNTKVAEAEIATMEKKGMATGLTAIHPLNGREVPIYIANFVLMDYGTGAVMAVPAHDQ 343
                                 G   I PL  R +PI       M+ GTG V   PAHD 
Sbjct: 245 --------------------LIGKYVILPLVNRRIPIVGDEHADMEKGTGCVKITPAHDF 284

Query: 344 RDFEFATKYGLDIIPVIKPADGSELDVSEAA--YTEKG---VLFAS---GEFDGLDFQAA 395
            D+E   ++ L +I ++   DG   D+ E+A  +  KG    +++S    EF  L+  AA
Sbjct: 285 NDYEVGKRHALPMINIL-TFDG---DIRESAQVFDTKGNESDVYSSEIPAEFQKLERFAA 340

Query: 396 FNAIAAKLEAEG----------------KGKKTVNFRLRDWGVSRQRYWGAPIPMVTTED 439
             A+ A ++A G                +G   +   L D    R      P  +   E+
Sbjct: 341 RKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP-AVEAVEN 399

Query: 440 GQVHPVP-------------------------ADQLPVILPEDVVMDGVTSPIKADKEWA 474
           G +  VP                           ++P    E      V      D+   
Sbjct: 400 GDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDE---AGNVYVGRNEDEVRK 456

Query: 475 KTTFNGEPALR-ETDTFDTFMESS-WYYARYCSPQADDILDPEKANYWLPVDQYIGGIEH 532
           +     +  LR + D  DT+  S+ W ++    P+  D L       + P    + G + 
Sbjct: 457 ENNLGADVVLRQDEDVLDTWFSSALWTFSTLGWPENTDAL-----RQFHPTSVMVSGFD- 510

Query: 533 ACMHLLYSRFFHKLLRDAGYVKSDE-----PFKKLLCQGMVLADAFYYTNDKGGK----- 582
               +++      ++    ++K +      PF  +   G++  D     +   G      
Sbjct: 511 ----IIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL 566

Query: 583 ---EWVSPTEVKVERDGKGRIVSAVDATGRQVEHSGMIKMSKSKNNGIDPQEMVDKYGAD 639
              + +S  E+  +R G        D   ++ E        K   NGI+P      +G D
Sbjct: 567 DMVDGISLPELLEKRTGNMMQPQLADKIRKRTE--------KQFPNGIEP------HGTD 612

Query: 640 TVRLFMMFASPADMTLEWQESGVEGANRFLRRVW---KLVREHTE------------LGQ 684
            +R  +   +     + W    +EG   F  ++W   + V  +TE            L  
Sbjct: 613 ALRFTLAALASTGRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSL 672

Query: 685 APALDASALNADQKALRRDVHKTIAKVTDDVARRQTFNTAIAAIMELMNKLTKAPMTEAQ 744
           A     +  N   KA R  +      +   +    T+N      +EL   +     TEA+
Sbjct: 673 ADRWILAEFNQTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGG-TEAE 731

Query: 745 ---DRAILDEALKAITLMLYPITPHICFEMW------VALGQSNIDTASWPTY-----DE 790
               R  L   L+ +  + +PI P I   +W        +    I    +P Y     DE
Sbjct: 732 LRGTRHTLVTVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDE 791

Query: 791 AALVEDEKL 799
           AAL + E L
Sbjct: 792 AALADTEWL 800