Pairwise Alignments

Query, 949 a.a., isoleucine--tRNA ligase from Vibrio cholerae E7946 ATCC 55056

Subject, 926 a.a., Isoleucyl-tRNA synthetase (EC 6.1.1.5) from Variovorax sp. SCN45

 Score =  794 bits (2051), Expect = 0.0
 Identities = 414/949 (43%), Positives = 589/949 (62%), Gaps = 38/949 (4%)

Query: 1   MSEYKDTLNLPETGFPMRGDLAKREPEMLQRWYQEDLYGAIRQAKKGKKSFVLHDGPPYA 60
           M + KDTLNL +T FPM+GDL  REPE+++RW ++ +Y  +R A  G+  F+LHDGPPYA
Sbjct: 1   MPDLKDTLNLLKTPFPMKGDLPTREPELVKRWAEQRVYERMRAAAAGRPPFILHDGPPYA 60

Query: 61  NGDIHIGHALNKILKDVIIKSKTLSGFDAPYIPGWDCHGLPIELMVEKKVGKPGQKVTAA 120
           NGDIHIGHA+NK+LKD++ KS+   GFD+ +IPGWDCHG+PIE  +E+  G+    +   
Sbjct: 61  NGDIHIGHAVNKVLKDMVFKSRFFDGFDSQWIPGWDCHGMPIEHRIEQTNGRG---LPTE 117

Query: 121 EFREKCREYAAGQVEGQKESFKRLGILGEWDKPYRTMDFVTEANIIRALGKIADNGHLLK 180
           + ++ CR+YA  Q + QK+ F RLG+LG+WD+ +RTMDF TEAN IR L +I   G L +
Sbjct: 118 QVQQLCRDYALQQTQSQKKDFLRLGLLGDWDRAFRTMDFETEANEIRFLDRIRQRGLLFR 177

Query: 181 GFKPVHWCTDCGSALAEAEVEYKNKVSPSIDVRFKAADEAAVLAKFGLAAGHEGKGDVSI 240
           G KPV+WC DC SALAEAE+EY  K S +  V   A D     ++F       GK    +
Sbjct: 178 GQKPVNWCVDCQSALAEAELEYAPKRSTTAYVGLLALDAVDFASRFKCQP-MPGK-QARL 235

Query: 241 VIWTTTPWTLPANRAVCLRADLEYVLIQVEGEQPERIIVASELAKSVMDRAGIEHFHNLG 300
            IWTTTPWTLP N AV L     Y L          +++A+E    ++   GI+H     
Sbjct: 236 TIWTTTPWTLPGNVAVGLDPRASYGLYDTPNGM---LVMAAETGGKLLAGLGIDH-ELCA 291

Query: 301 FATGADLELVQFQHPFYSFTVPAILGDHVTTDSGTGVVHTAPGHGQEDFAVGQQYGLEVA 360
            A G DL  ++ +HP     VP +  D V+ D+GTG+VH AP HG EDF + +  G+   
Sbjct: 292 VAPGLDLVGLRLKHPLLDTEVPLVAADFVSLDTGTGLVHLAPAHGAEDFELCRSLGIGGE 351

Query: 361 NPVGSNGVYLPDTELFAGQHVFKANDSVLEVLKEKGALLHHHAYEHSYPHCWRHKTPIIF 420
           N +   G ++    +  G  + + +  +L++L+  G+LL     EHSYPHCWRHKTPI+F
Sbjct: 352 NVIDGAGRFVAGLPMVGGMGLEEGSKLILDMLQADGSLLKQERIEHSYPHCWRHKTPILF 411

Query: 421 RATPQWFVSMEQAG------LREQALTAIKGVHWMPDWGQSRIEGMVAGRPEWCISRQRT 474
           R+T QWF+ M++        LRE A  AI+ V + P  G+SR+E M+ GRP+WC+SRQRT
Sbjct: 412 RSTTQWFIGMDRRATGDTRTLRETAREAIRDVPFYPASGRSRMEAMIDGRPDWCVSRQRT 471

Query: 475 WGVPIALFVHKETAELHPNSADLIEKVAQLVEQKGIQAWWDLDTAELLGAEDAANYEKVL 534
           WGVP+  FV +    LHP +A L+E+VA  +E++GI AW  L  A+L    D A YEK+ 
Sbjct: 472 WGVPLPFFVRRSDKSLHPQTARLVEEVALRIEREGISAWTRLKPADL--GVDEAEYEKLS 529

Query: 535 DTLDVWFDSGVTHSAVV-DARQ-EFNGAEADMYLEGSDQHRGWFQSSLISSVAMKGKAPY 592
           DTLDVWFDSG  H+ V  DAR+ + +G  AD+YLEGSDQHRGWF SSL++  A   +AP+
Sbjct: 530 DTLDVWFDSGSIHATVYRDARRPDAHGYPADLYLEGSDQHRGWFGSSLMTGCAADARAPF 589

Query: 593 KEVLTHGFVVDGQGRKMSKSIGNVVAPQDVTNKLGADILRLWVASTDYTGEVAVSDEILK 652
           K VLTHGF VDG G+KMSKS+GN V+PQ V +  GADI+RLW+ASTDY+ +++VS+EIL 
Sbjct: 590 KAVLTHGFAVDGDGKKMSKSLGNTVSPQQVASTRGADIIRLWIASTDYSTDISVSEEILD 649

Query: 653 RSADAYRRIRNTARFFLANLNGFNPTTDIIPVEDMVALDRWAVGRALAAQQEIIQAYQDY 712
           R  + YRRIRNT RF L N++ F+ T D +  +D+ ++D++A+ R     ++  ++Y++Y
Sbjct: 650 RVVEMYRRIRNTIRFLLLNVSDFDATADCMRAQDLESVDQYAMLRCRELAEQCRKSYREY 709

Query: 713 NLHAVVQRLMNFCSIEMGSFYLDVIKDRQYTAKRGGHAQRSCQTALFFIVEALVRWMAPI 772
           +  AV + L  +C+ E+G FYLDV+KDR Y + R    +RS QTAL  I++ L+   API
Sbjct: 710 DFVAVTRLLHGYCADELGGFYLDVLKDRLYASARDSRERRSAQTALHAILKNLLLLTAPI 769

Query: 773 MSFTADEIWNAMPAQQADGSARDKFVFTTEWFDGLFGLAEGEELNNAFWNDIQKVRGSVN 832
           +SFTA+E W  +   Q D       VF   W + +    +  +++  FW  ++ +R +V 
Sbjct: 770 LSFTAEEAWAVLFKSQEDS------VFVHIWDEAMPPRIDDSKVSE-FWRQVRLLRPNVM 822

Query: 833 KLLENARNEKLIGGSLQAELVLFADDSLASKLAKLGDELRFVLLTSKAVVKPLAEKSEAA 892
           K +E+ R+   IG S +A +V+ A   +   LA LG EL  + + +   +K         
Sbjct: 823 KAIEDIRSSGAIGRSSEASIVIEAPVGIVESLALLGSELPSIFMVADVALK--------- 873

Query: 893 QATDIDGLFVQVNKTEAEKCDRCWHHTPDVGTIAGHTTICGRCVSNVEG 941
              + D + V+V +T + +C RCW H   V   A    +CGRC + + G
Sbjct: 874 ---ESDAVEVKVRRTSSLRCMRCWRHDATVTDDAEQKMLCGRCRTVLSG 919