Pairwise Alignments

Query, 949 a.a., isoleucine--tRNA ligase from Vibrio cholerae E7946 ATCC 55056

Subject, 951 a.a., valine--tRNA ligase from Escherichia coli ECRC62

 Score =  130 bits (327), Expect = 4e-34
 Identities = 186/880 (21%), Positives = 326/880 (37%), Gaps = 192/880 (21%)

Query: 25  EPEMLQRWYQEDLYGAIRQAKKGKKSFVLHDGPPYANGDIHIGHALNKILKDVIIKSKTL 84
           E  + + W ++  +       + ++SF +   PP   G +H+GHA  + + D +I+ + +
Sbjct: 11  EQPLYEHWEKQGYFKP--NGDESQESFCIMIPPPNVTGSLHMGHAFQQTIMDTMIRYQRM 68

Query: 85  SGFDAPYIPGWDCHGLPIELMVEKKV----GKPGQKVTAAEFREKCREYAAGQVEGQKES 140
            G +  +  G D  G+  +++VE+K+    GK         F +K  E+ A         
Sbjct: 69  QGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTRHDYGREAFIDKIWEWKAESGGTITRQ 128

Query: 141 FKRLGILGEWDKPYRTMDFVTEANIIRALGKIADNGHLLKGFKPVHWCTDCGSALAEAEV 200
            +RLG   +W++   TMD      +     ++     + +G + V+W     +A+++ EV
Sbjct: 129 MRRLGNSVDWERERFTMDEGLSNAVKEVFVRLYKEDLIYRGKRLVNWDPKLRTAISDLEV 188

Query: 201 EYKNKVSPSIDVRFKAADEAAVLAKFGLAAGHEGKGDVSIVIWTTTPWTLPANRAVCLRA 260
           E +        +R+  AD A               G   +V+ TT P TL  +  V +  
Sbjct: 189 ENRESKGSMWHIRYPLADGAKT-----------ADGKDYLVVATTRPETLLGDTGVAVN- 236

Query: 261 DLEYVLIQVEGEQPERIIVASELAKSVMDRAGIEHFHNLGFATGADLELVQFQHPFYSFT 320
                        PE       + K V+                          P  +  
Sbjct: 237 -------------PEDPRYKDLIGKYVI-------------------------LPLVNRR 258

Query: 321 VPAILGDHVTTDSGTGVVHTAPGHGQEDFAVGQQYGLEVANPVGSNGVYLPDTELFAGQ- 379
           +P +  +H   + GTG V   P H   D+ VG+++ L + N +  +G      ++F  + 
Sbjct: 259 IPIVGDEHADMEKGTGCVKITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKG 318

Query: 380 -----------------HVFKANDSVLEVLKEKGALLHHHAYEHSYPHCWRHKTPIIFRA 422
                              F A  +V+  +   G L     ++ + P+  R    I    
Sbjct: 319 NESDVYSSEIPAEFQKLERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPML 378

Query: 423 TPQWFVSMEQAGLREQALTAIKG--VHWMPDWGQSRIEGMVAGRPEWCISRQRTWGVPIA 480
           T QW+V  +   L + A+ A++   + ++P   ++     +    +WCISRQ  WG  I 
Sbjct: 379 TDQWYVRADV--LAKPAVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIP 436

Query: 481 LFVHKETAELHPNSADLIEKVAQLVEQKGIQAWWDLDTAELLGAEDAANYEKVLDTLDVW 540
            +  +        + D + K                     LGA+ A   ++  D LD W
Sbjct: 437 AWYDEAGNVYVGRNEDEVRK------------------ENNLGADVALRQDE--DVLDTW 476

Query: 541 FDSGV-THSAV-----VDARQEFNGAEADMYLEGSDQHRGWFQSSLISSVAM------KG 588
           F S + T S +      DA ++F+     + + G D    W    ++ ++        K 
Sbjct: 477 FSSALWTFSTLGWPENTDALRQFH--PTSVMVSGFDIIFFWIARMIMMTMHFIKDENGKP 534

Query: 589 KAPYKEVLTHGFVVDGQGRKMSKSIGNVVAPQDVTNKL---------------------- 626
           + P+  V   G + D +G+KMSKS GNV+ P D+ + +                      
Sbjct: 535 QVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPLDMVDGISLPELLEKRTGNMMQPQLADKI 594

Query: 627 ---------------GADILRLWVASTDYTGEVAVSDEILKRSADAYR----RIRNTARF 667
                          G D LR  +A+   TG     D  +KR  + YR    ++ N +RF
Sbjct: 595 RKRTEKQFPNGIEPHGTDALRFTLAALASTGRDINWD--MKR-LEGYRNFCNKLWNASRF 651

Query: 668 FLANLN----GFNPTTDIIPVEDMVALDRWAVGRALAAQQEIIQAYQD----YNLHAVVQ 719
            L N      GFN     + +      DRW     LA   + I+AY++    +       
Sbjct: 652 VLMNTEGQDCGFNGGEMTLSL-----ADRW----ILAEFNQTIKAYREALDSFRFDIAAG 702

Query: 720 RLMNFCSIEMGSFYLDVIKDRQYTAKRGGHAQ-RSCQTALFFIVEALVRWMAPIMSFTAD 778
            L  F   +   +YL++ K        G  A+ R  +  L  ++E L+R   PI+ F  +
Sbjct: 703 ILYEFTWNQFCDWYLELTKP---VMNGGTEAELRGTRHTLVTVLEGLLRLAHPIIPFITE 759

Query: 779 EIWNAM--------------PAQQADGSARDKFVFT-TEW 803
            IW  +              P  Q D S  D+     TEW
Sbjct: 760 TIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTEW 799