Pairwise Alignments
Query, 949 a.a., isoleucine--tRNA ligase from Vibrio cholerae E7946 ATCC 55056
Subject, 947 a.a., Isoleucine--tRNA ligase from Azospirillum sp. SherDot2
Score = 800 bits (2066), Expect = 0.0
Identities = 430/963 (44%), Positives = 581/963 (60%), Gaps = 54/963 (5%)
Query: 3 EYKDTLNLPETGFPMRGDLAKREPEMLQRWYQEDLYGAIRQAKKGKKSFVLHDGPPYANG 62
+YK T+ LP T FPMRG L +EPE+L+RW + DL+G +R KG+K FVLHDGPPYANG
Sbjct: 4 DYKSTVFLPRTDFPMRGGLPTKEPELLKRWAEMDLFGKLRAGAKGRKKFVLHDGPPYANG 63
Query: 63 DIHIGHALNKILKDVIIKSKTLSGFDAPYIPGWDCHGLPIELMVEKKVGKPGQK---VTA 119
+IHIGHA+NKILKDVI++ + + GFDA Y+PGWDCHGLPIE +E+K K G+ V
Sbjct: 64 NIHIGHAVNKILKDVIVRFRQMQGFDADYVPGWDCHGLPIEWKIEEKYRKAGKNKDDVPL 123
Query: 120 AEFREKCREYAAGQVEGQKESFKRLGILGEWDKPYRTMDFVTEANIIRALGKIADNGHLL 179
+FR +CR++A V Q F+RLG+ G+W +PY TM F EA I+R + K A NG L
Sbjct: 124 IQFRAECRQFAEEWVGIQAAEFRRLGVEGDWKQPYATMTFPAEAQIVREIHKFAVNGGLY 183
Query: 180 KGFKPVHWCTDCGSALAEAEVEYKNKVSPSIDVRFKAADEAAVLAKFGLAAGHEGKGDVS 239
KGFKPV W +ALA+AEVEY + S ++ RF +A + S
Sbjct: 184 KGFKPVMWSVVEKTALADAEVEYHDHTSTTVFARFPVWGSSAPEVD-----------EAS 232
Query: 240 IVIWTTTPWTLPANRAVCLRADLEYVLIQV----EGEQP---ERIIVASELAKSVMDRAG 292
IVIWTTTPWTLP NRA+ ++EY +V EG + ER+++A+ LA+SV+ G
Sbjct: 233 IVIWTTTPWTLPGNRAIAYGDEIEYATFKVTAVAEGSKAVVGERLVLATTLAESVLKETG 292
Query: 293 IEHFHNLGFATGADLELVQFQHPF----YSFTVPAILGDHVTTDSGTGVVHTAPGHGQED 348
I + L G+ L +HP Y F VP + GD VTTD+G+G VH APGHG++D
Sbjct: 293 ITEWKQLHRFPGSRLAGTYCRHPLNGKGYDFKVPLLAGDFVTTDAGSGFVHIAPGHGEDD 352
Query: 349 FAVGQQYGLEVANPVGSNGVYLPDTELFAGQHVFK-------ANDSVLEVLKEKGALLHH 401
F +GQ +EV VG +G Y LFAG V+ AN +VLE +E G+LL
Sbjct: 353 FRLGQANNIEVPQTVGEDGRYYAHVPLFAGLAVYTDQGKTGPANKAVLEAFEECGSLLAS 412
Query: 402 HAYEHSYPHCWRHKTPIIFRATPQWFVSMEQAGLREQALTAIKGVHWMPDWGQSRIEGMV 461
+ +HSYPH WR K P+IFR TPQWF+SME LR+ AL AI W+P G++RI M+
Sbjct: 413 NRIKHSYPHSWRSKAPLIFRTTPQWFISMETNDLRKVALQAISDTRWVPSQGENRIRSMI 472
Query: 462 AGRPEWCISRQRTWGVPIALFVHKETAELHPNSADLIEKVAQLVEQKGIQAWWDLDTAEL 521
RP+WCISRQR WGVPIALFV K T E+ + A + ++A + +Q+G AW+ D +
Sbjct: 473 ESRPDWCISRQRAWGVPIALFVRKSTGEILRDEA-VFARIADIFQQEGADAWFARDPQDF 531
Query: 522 LGAEDAA-NYEKVLDTLDVWFDSGVTHSAVVDARQEFNGAEADMYLEGSDQHRGWFQSSL 580
LG AA ++E+V D +DVWF+SG +H+ V++ R E AD+YLEGSDQHRGWF SSL
Sbjct: 532 LGNSYAAEDFEQVRDIVDVWFESGSSHAFVLEQRLEELAWPADLYLEGSDQHRGWFHSSL 591
Query: 581 ISSVAMKGKAPYKEVLTHGFVVDGQGRKMSKSIGNVVAPQDVTNKLGADILRLWVASTDY 640
+ S +G+APY VLTHGFV+D QGRKMSKS+GNVVAPQ+V ++ GADILRLW+ ++DY
Sbjct: 592 LESCGTRGRAPYNAVLTHGFVLDEQGRKMSKSLGNVVAPQEVCDQYGADILRLWIINSDY 651
Query: 641 TGEVAVSDEILKRSADAYRRIRNTARFFLANLNGFNPTTDIIPVEDMVALDRWAVGRALA 700
+ ++ + EI+K AD YRR+RNT R+ L L + P + I +M L+RW + R
Sbjct: 652 SEDLRIGKEIIKTQADLYRRLRNTLRYLLGALADYTP-AERIDAAEMPELERWVLHRLSE 710
Query: 701 AQQEIIQAYQDYNLHAVVQRLMNFCSIEMGSFYLDVIKDRQYTAKRGGHAQRSCQTALFF 760
+ + + Y+ HA+ L NFC++E+ +FY DV KD Y +RS +T +
Sbjct: 711 LDTLVREKVEAYDFHALFVALHNFCAVELSAFYFDVRKDSLYCDAVDSVRRRSVRTVMEH 770
Query: 761 IVEALVRWMAPIMSFTADEIWNAMPAQQADGSARDKFVFTTEWFD---GLFGLAE--GEE 815
+ L W+API+ FTA+E W A P +G+ W D L G E E
Sbjct: 771 LFSNLTAWLAPILCFTAEEAWLARPEGLTEGAG---------WTDESVHLRGFPEVPAEW 821
Query: 816 LNNAF---WNDIQKVRGSVNKLLENARNEKLIGGSLQAELVLFADDSLASKLAKLGDELR 872
N+A W ++ VR V LE R K IG SLQA+ +F D + + LA + + +
Sbjct: 822 RNDALASKWEAVRTVRRVVTGALELERANKAIGSSLQADPTVFVDSATKALLADV--DFQ 879
Query: 873 FVLLTSKAVVKPLAEKSEAAQATDIDGLFVQVNKTEAEKCDRCWHHTPDVGTIAGHTTIC 932
V +TS V A A D+ G+ V + EKC+RCW +VGT+ H T+C
Sbjct: 880 DVCITSAIEVTDAAAPEGAFTLPDVPGIAVAPALADGEKCERCWKVLEEVGTVHDHPTLC 939
Query: 933 GRC 935
RC
Sbjct: 940 IRC 942