Pairwise Alignments
Query, 559 a.a., PqiB family protein from Pseudomonas syringae pv. syringae B728a
Subject, 877 a.a., MCE domain protein from Escherichia coli BL21
Score = 182 bits (463), Expect = 4e-50
Identities = 105/323 (32%), Positives = 175/323 (54%), Gaps = 14/323 (4%)
Query: 9 PASVPRPEVKRRRLRVSLIWLVPIVAAIIGISMALHDWMNIGPRITVSFLTAEGLEANKT 68
PAS ++K +R R+S WL+P +A +I + + + G +T+ F++A+G+ +T
Sbjct: 6 PASTTEAQIKNKR-RISPFWLLPFIALMIAGWLIWDSYQDRGNTVTIDFMSADGIVPGRT 64
Query: 69 QVKYKNVVIGMVTDISLSEDRTHVLANIELNTSAAPFTRVDSQFWVVRPRIGAHGVSGVD 128
V+Y+ V +G V DISLS+D + + + + R ++QFW+V P+ GVSG+D
Sbjct: 65 PVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDALREETQFWLVTPKASLAGVSGLD 124
Query: 129 TLLSGAFIGADAGSAEETKSSFTGLETPPPVTFGEKGKRFTLHTDDLGSLDIGSPIYFRR 188
L+ G +IG G +E + F L+T P L DLGSL+ GS +YFR+
Sbjct: 125 ALVGGNYIGMMPGKGKE-QDHFVALDTQPKYRLDNGDLMIHLQAPDLGSLNSGSLVYFRK 183
Query: 189 IQVGQVVAYDLSKDGRGVDIQIFINAPNDQYITTDTRFWNASGVDITVGASGVKVNTQSL 248
I VG+V Y ++ + +GV I + I + +RFWN SGVD V SG KV +SL
Sbjct: 184 IPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKGSRFWNVSGVDANVSISGAKVKLESL 243
Query: 249 TSIISGGIAFREPNWSPDSKPADENAEFKIFDDQTAAMAPPDGEPRYIRMRFN-QSLRGL 307
++++G IAF P +SKPA+ F +++D + R + ++ S GL
Sbjct: 244 AALVNGAIAFDSPE---ESKPAEAEDTFGLYEDLAHSQ-------RGVIIKLELPSGAGL 293
Query: 308 TVNAA-VDFLGVNIGKVVSVDLD 329
T ++ + + G+ +G++ +DL+
Sbjct: 294 TADSTPLMYQGLEVGQLTKLDLN 316
Score = 82.4 bits (202), Expect = 7e-20
Identities = 67/235 (28%), Positives = 103/235 (43%), Gaps = 4/235 (1%)
Query: 49 IGPRITVSFLTAEGLEANKTQVKYKNVVIGMVTDISLSEDRTHVLANIELNTS-AAPFTR 107
+G +IT+ A G A ++Y + IG + + L R V A L F R
Sbjct: 633 VGGQITLHAFDA-GKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLYPEYVQTFAR 691
Query: 108 VDSQFWVVRPRIGAHGVSGVDTLLSGAFIGADAGSAEETKSSFTGLETPPPVTFGEKGKR 167
++F VV P+I A GV +DT+L +I + G + F E + G
Sbjct: 692 GGTRFSVVTPQISAAGVEHLDTILQ-PYINVEPGRGNPRRD-FELQEATITDSRYLDGLS 749
Query: 168 FTLHTDDLGSLDIGSPIYFRRIQVGQVVAYDLSKDGRGVDIQIFINAPNDQYITTDTRFW 227
+ + GSL IG+P+ FR ++VG V L V I + I+ + ++ FW
Sbjct: 750 IIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRYQHLVRNNSVFW 809
Query: 228 NASGVDITVGASGVKVNTQSLTSIISGGIAFREPNWSPDSKPADENAEFKIFDDQ 282
ASG + G +G V T + I GGIAF P +P + A E F + + +
Sbjct: 810 LASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGKHFLLQESE 864
Score = 55.8 bits (133), Expect = 7e-12
Identities = 62/287 (21%), Positives = 123/287 (42%), Gaps = 24/287 (8%)
Query: 53 ITVSFLTAEGLEANKTQVKYKNVVIGMVTDISLSEDRTHVLANIELNTSAAPFTRVDSQF 112
+T++ + G++A + + + V +G V D L+ V + + + DS+F
Sbjct: 395 LTLTAPESYGIDAGQPLILH-GVQVGQVIDRKLTSKG--VTFTVAIEPQHRELVKGDSKF 451
Query: 113 WVVRPRIGAH-GVSGVDTLLSGA---------FIGADAGSAEETKSSFTGLETPPPVTFG 162
VV R+ G+ GV+ L + A + D G + + + LE +
Sbjct: 452 -VVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENSLS 510
Query: 163 E-KGKRFTLHTDDLGSLDIGSPIYFRRIQVGQVVAYDLSKDGRGVDIQIFINAPNDQYIT 221
+ +L + L + GS + +R+ +VG+V+ + DI + I +T
Sbjct: 511 DLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVIT--VRPRANAFDIDLHIKPEYRNLLT 568
Query: 222 TDTRFWNASGVDITVGASGVKVNTQSLTSIISGGIAFREPNWSPDSKPADENAEFKIFDD 281
+++ FW G + + SG+ V L+ + G I+F N S S + + ++
Sbjct: 569 SNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISF--DNLSGASASQRKGDKRILYAS 626
Query: 282 QTAAMAPPDGEPRYIRMRFNQSLRGLTVNAAVDFLGVNIGKVVSVDL 328
+TAA A + F+ L V + +LG++IG++ ++DL
Sbjct: 627 ETAARAVGG---QITLHAFDAG--KLAVGMPIRYLGIDIGQIQTLDL 668
Score = 32.3 bits (72), Expect = 8e-05
Identities = 34/151 (22%), Positives = 66/151 (43%), Gaps = 25/151 (16%)
Query: 70 VKYKNVVIGMVTDISLSEDRTHVLANIELNTSAAPFTRVDSQFWVVRPRIGAHGVSGVDT 129
V ++ + +G V D +++ ++ V+ ++ + + S+FW VSGVD
Sbjct: 179 VYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKGSRFW---------NVSGVDA 229
Query: 130 --LLSGAFIGADAGSAEETKS-SFTGLETPPPV----TFG--------EKGKRFTLHTDD 174
+SGA + ++ +A + +F E P TFG ++G L
Sbjct: 230 NVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAHSQRGVIIKLELPS 289
Query: 175 LGSLDIGS-PIYFRRIQVGQVVAYDLSKDGR 204
L S P+ ++ ++VGQ+ DL+ G+
Sbjct: 290 GAGLTADSTPLMYQGLEVGQLTKLDLNPGGK 320