Pairwise Alignments

Query, 532 a.a., ATP-dependent DNA ligase (EC 6.5.1.1) LigC from Sphingobium sp. HT1-2

Subject, 573 a.a., ATP-dependent DNA ligase from Methanococcus maripaludis S2

 Score =  121 bits (303), Expect = 8e-32
 Identities = 100/411 (24%), Positives = 183/411 (44%), Gaps = 25/411 (6%)

Query: 141 KLATGGLRVGISARLAKTGSAQAFGLDVDDVEQVWHALAPPYAALFD-WAEGRSGRPDPE 199
           +L    +R+G++        +  F ++ + VE+++ A+      L +    G     DPE
Sbjct: 159 RLILEDMRIGMNIPTILASFSNYFNVNKESVEKIY-AVTNDIGLLGEKLISGSDIENDPE 217

Query: 200 -GTPYFRPF-----MLAHPLEADSVDLADYAAEWKWDGIRIQIVGTGRETRLYSRAGDDI 253
                FRP       L   +E   ++      E K+DG R+Q+  +  + ++YSR  ++I
Sbjct: 218 LKLKVFRPIKPMLAQLTPSIEDAMIETKMPQFETKYDGARVQVHKSNGDVKIYSRRLENI 277

Query: 254 TGSFPEIAQAFTGHAVLDGELLVKGEFQGAKEHGGAAASFNALQQRLGRKAVSAKMMADY 313
           T S PE+ +      +    ++++GE        G    F  + +R  RK    KM    
Sbjct: 278 TNSVPELVEEIKKLDI--DNIILEGECVAMDLDSGKPRPFQDILRRFRRKYNIDKMAEKI 335

Query: 314 PAFVRLYDLLIEGDDDLRALPWTQRRARLE---------AYMAQLSPDRFDLSAV----- 359
              +  +D+L   +  L   P   RR  LE         + + ++  + F    +     
Sbjct: 336 ALRIYFFDVLYY-NRGLIDTPLKTRREILEKLFGTNNWDSELEKIKKEIFSSKMLFSSFK 394

Query: 360 IDAPDFAALADIRAGARDAAIEGVMLKRRDSPYVAGRKAALWYKWKRDPLTADCVMMYAQ 419
           +++ D   + +    +     EG+M+K  D+PY  G +    YK K      D V+  A+
Sbjct: 395 LNSGDPNLVKEFFNWSLSIGHEGIMIKNPDAPYTPGSRVKTMYKVKPTLENLDVVVTRAK 454

Query: 420 RGHGKRSSFYSDYTFGCWTEDGELLPVGKAYSGFTDEELKWLDRFVRGNTVNRFGPVREV 479
            G GKR  +Y  Y       DG L  +G   SG T+++L+ L + V    +   G    +
Sbjct: 455 IGMGKRKDWYGSYELSVKDNDGNLHVIGNVGSGLTEDDLEKLTKIVNEIKIEDLGEEVIL 514

Query: 480 EKTLVLEVAFDSIHDSKRHKSGLAMRFPRIARIRRDKPAAEADTVEGLKRL 530
           E  +VLEV ++ I  S++++ G A+RFPR+ +IR DK   + +T++ +K++
Sbjct: 515 EPKIVLEVTYEEIQTSEKYEMGYALRFPRVVQIREDKSINDINTLDDVKKI 565