Pairwise Alignments

Query, 355 a.a., ATP-dependent DNA ligase from Sinorhizobium meliloti 1021

Subject, 532 a.a., ATP-dependent DNA ligase (EC 6.5.1.1) LigC from Sphingobium sp. HT1-2

 Score = 67.0 bits (162), Expect = 1e-15
 Identities = 97/343 (28%), Positives = 138/343 (40%), Gaps = 71/343 (20%)

Query: 33  PMEARSATELPGGDAWQYEPKWDGFRCLAFKSGDEVDIRAKSGKPLGRFFPEIVALLRRL 92
           P+EA S         W    KWDG R     +G E  + +++G  +   FPEI       
Sbjct: 212 PLEADSVDLADYAAEW----KWDGIRIQIVGTGRETRLYSRAGDDITGSFPEIAQAF--- 264

Query: 93  EPDMFVLDGELVIEVD---------GRLSFDALQMRLHPAASRVQKLSQETPAKLILFDM 143
                VLDGEL+++ +            SF+ALQ RL   A    K+  + PA + L+D+
Sbjct: 265 -TGHAVLDGELLVKGEFQGAKEHGGAAASFNALQQRLGRKAVSA-KMMADYPAFVRLYDL 322

Query: 144 LAGTDGTILTGEPLQTRRACLEAFAKKNAVAEMLESSPFTLDIK---EAERW--LTSWEA 198
           L   D   L   P   RRA LEA+        M + SP   D+    +A  +  L    A
Sbjct: 323 LIEGDDD-LRALPWTQRRARLEAY--------MAQLSPDRFDLSAVIDAPDFAALADIRA 373

Query: 199 GATD----GVVAKRRDGVYECGERAMVKVKRLK---TADCVV--GGFRYESDSSLVGSLL 249
           GA D    GV+ KRRD  Y  G +A +  K  +   TADCV+      +   SS      
Sbjct: 374 GARDAAIEGVMLKRRDSPYVAGRKAALWYKWKRDPLTADCVMMYAQRGHGKRSSFYSDYT 433

Query: 250 LGLYDTEGLLNHVGFTATISDKERPALTNQLEALREPPGFTGKAPGGPSRW----SSERS 305
            G +  +G L  VG                    +   GFT +      R+    +  R 
Sbjct: 434 FGCWTEDGELLPVG--------------------KAYSGFTDEELKWLDRFVRGNTVNRF 473

Query: 306 GEWEPVRPELVVEVRFDHV-SNRRFRHG-----TKLMRWRPDK 342
           G    V   LV+EV FD +  ++R + G      ++ R R DK
Sbjct: 474 GPVREVEKTLVLEVAFDSIHDSKRHKSGLAMRFPRIARIRRDK 516