Pairwise Alignments
Query, 1621 a.a., NAD-specific glutamate dehydrogenase from Pseudomonas putida KT2440
Subject, 1613 a.a., NAD-glutamate dehydrogenase from Vibrio cholerae E7946 ATCC 55056
Score = 1396 bits (3614), Expect = 0.0
Identities = 731/1590 (45%), Positives = 1022/1590 (64%), Gaps = 16/1590 (1%)
Query: 30 PQVALFAEQFFGIISLDELTQRRLSDLAGCTLSAWRIIERFDPEYPQVRVYNPDYERNGW 89
P V + F IS D+L +R SDL G LS W I + VRV+NP R GW
Sbjct: 29 PLVTQLGQHLFSNISQDDLVERNESDLYGAVLSLWHHINEKKADERSVRVFNPTVSRQGW 88
Query: 90 QSTHTVVEVLHHDLPFLVDSVRTELNRRGYSIHTLQTTVLSVRRGAKGELLELLPKGTQG 149
QSTHT+VE++ D PFLVDS++ L+R G + H + + R G + + QG
Sbjct: 89 QSTHTIVEIVLPDSPFLVDSIKMALSRLGLASHLMLNGPAHIARHDDGSIKSI----NQG 144
Query: 150 EGVRYESLMYLEIDRCANAAELTVLTREIEQVLAEVRVVVADFEPMKAKLREVVAQVEQT 209
EG + S+ ++E+DR ++ E+T L E+ +L + +VV D++PM KL +V+ Q+E
Sbjct: 145 EG-QLTSMFHIEVDRLSSKEEMTELKNELLDILHDTALVVKDWKPMATKLEQVINQLEAD 203
Query: 210 AFG-PAQNEK-GEVKAFLEWLLDNHFTFLGYEEFTVKGDADGGQMVYDEQSFLGLPRRLR 267
P + E+ E FL WL +++FTF+GY+EF + ++ + + LGL
Sbjct: 204 KKQIPVEAERLQETIQFLRWLGNHNFTFMGYKEFDLVEKNGDTELTPTKDTGLGLFSDNE 263
Query: 268 VGLTAEELRIEDYAVAYLNEPLLLSFAKAALPSRVHRPAYPDYVSIRQLDADGKVIKEHR 327
+ + + D A +P LL K SR+HRPAY DY+ I++ DA GKVI EHR
Sbjct: 264 RVRSVKLSQFPDSARLEAKKPFLLILTKGNKQSRIHRPAYTDYIGIKKFDAKGKVIGEHR 323
Query: 328 FMGLYTSSVYGESVHAIPYIRVKVAEVERRSGFDPKAHLGKELAQVLEVLPRDDLFQTPI 387
F GLYTS+VY +SV IP IR KV + SG+ ++ K L +LE PRD+L Q
Sbjct: 324 FTGLYTSAVYNQSVEGIPLIREKVGRILAASGYRQGSYAYKALHNILENYPRDELLQARE 383
Query: 388 DELFSTVMAIVQIQERNKIRVFLRKDPYGRFCYCLAYVPREIYSTEVRQKIQQVLMERLK 447
+EL M +VQ+Q+R+ +R+F+RKDP+GRF C+ YV +E Y+TE+R+K QQV +
Sbjct: 384 EELLEVGMGVVQMQDRDLLRLFVRKDPFGRFFSCMVYVTKERYNTELRRKTQQVFKQYFG 443
Query: 448 AS-DCEFWTFFSESVLARVQLILRVDPKNRIDIDPQQLEREVIQACRSWHDDYSALVVEN 506
D EF T+FSES LAR I+RVD N I++D +++E+ +++A SW D + +V N
Sbjct: 444 CEQDVEFTTYFSESPLARTHYIVRVD-NNNINVDVKKIEQNLMEASTSWDDRLAEAIVAN 502
Query: 507 FGEAQGTNILADFPKGFPAGYRERFAAHSAVVDLQHVLNLSESKPLAMSFYQ-PLTQVGE 565
FGE++G + ++ + FP Y+E SA+ D++H+ L E L M FY+ T
Sbjct: 503 FGESRGLPLSKEYQRAFPRSYKEDVMPGSALADIEHLEALDEHNKLGMLFYRLQETAKDS 562
Query: 566 RILHCKLYHADTPLALSDVLPILENLGLRVLGEFPYRLRHANGREYWIHDFAFTYSEGLS 625
+ + KLYH D P+ LSDV+P+LENLGLRV+GE PY + ANG+ YWI DF+ +
Sbjct: 563 KAVRLKLYHKDEPIHLSDVMPMLENLGLRVIGESPYEVVKANGQVYWILDFSMLHKSDKQ 622
Query: 626 LDIQQLNDTLQDAFIHIVRGDAENDAFNRLVLTAGLPWRDVALLRAYARYLKQIRLGFDL 685
+D+++ D Q AF I G+ E+D FNRL+L A L R+V++LRAYARY++Q+ F
Sbjct: 623 VDLREARDRFQQAFAAIWAGELESDGFNRLILGASLSGREVSILRAYARYMRQVGFPFSQ 682
Query: 686 GYIASTLNNHTDIARELTRLFKTRFYLARKLTQDDLDDKQQRLEQAILSALDDVQVLNED 745
YI TL++H D+A+ L LF RF K + Q + +++ LD VQ L++D
Sbjct: 683 HYIEDTLSHHPDLAQGLVDLFVRRFDPKYKGGEKG----QAEIIKSLTEQLDQVQSLDDD 738
Query: 746 RILRRYLDLIKATLRTNFYQPDANGQNKSYFSFKFNPKLIPELPKPVPKFEIFVYSPRVE 805
RI+RRY+++I ATLRTN+YQ D + QNK + S K P IPE+P PVP FEIFVY+P +E
Sbjct: 739 RIIRRYMEMINATLRTNYYQLDEHKQNKPWLSLKMKPSEIPEIPAPVPAFEIFVYAPDIE 798
Query: 806 GVHLRFGNVARGGLRWSDREEDFRTEVLGLVKAQQVKNSVIVPVGAKGGFLPRRLPLGGS 865
GVHLR G VARGGLRWSDR+EDFRTE+LGLVKAQQVKN+VIVPVGAKGGF+ ++ L +
Sbjct: 799 GVHLRGGKVARGGLRWSDRQEDFRTEILGLVKAQQVKNTVIVPVGAKGGFVCKKQYLYTT 858
Query: 866 RDEIAAEGVACYRIFISGLLDITDNLKDGGVVPPANVVRHDDDDPYLVVAADKGTATFSD 925
RDEI AEG CY+ FI LLD+TDN+ +G VVPP NVVRHD+DDPYLVVAADKGTATFSD
Sbjct: 859 RDEIFAEGQRCYKRFIRALLDVTDNIIEGQVVPPKNVVRHDEDDPYLVVAADKGTATFSD 918
Query: 926 IANGIAIDYGFWLGDAFASGGSAGYDHKKMGITARGAWVGVQRHFRERGINVQEDPITVI 985
+AN ++ +Y FWLGDAFASGGS GYDHK MGITA+G W V+RHFRE GI+ Q T I
Sbjct: 919 LANSVSAEYQFWLGDAFASGGSNGYDHKAMGITAKGGWESVKRHFREMGIDCQTTDFTAI 978
Query: 986 GVGDMAGDVFGNGLLMSDKLQLVAAFNHLHIFIDPNPDPATSFAERKRLFDLPRSAWSDY 1045
G+GDMAGDVFGNG+L+S ++L+AAFNH+HIFIDP PD A+S+ ER RLF+LPRS+W DY
Sbjct: 979 GIGDMAGDVFGNGMLLSKHIRLLAAFNHIHIFIDPTPDSASSWEERNRLFNLPRSSWEDY 1038
Query: 1046 DTSIMSEGGGIFPRSAKSIAISPQMKERFAIEADRLTPTELLNALLKAPVDLLWNGGIGT 1105
+ ++S+GGG+F R AK+I ++P+M++ + L P EL+ +LK VDLLWNGGIGT
Sbjct: 1039 NPKLISKGGGVFSRKAKAITLTPEMQKMLNTKKTTLAPNELIKMILKMEVDLLWNGGIGT 1098
Query: 1106 YVKASTESHADVGDKANDALRVNGNELRCKVVGEGGNLGMTQLGRVEFGLNGGATNTDFI 1165
YVK+S E+H DVGD+AND LRV+G E+ K++GEGGNLGMTQ GR+EF L GG NTDF+
Sbjct: 1099 YVKSSIETHTDVGDRANDGLRVDGREVNAKIIGEGGNLGMTQRGRIEFALKGGRVNTDFV 1158
Query: 1166 DNAGGVDCSDHEVNIKILLNEVVQGGDMTEKQRNQLLGSMTDEVAGLVLGNNYKQTQALS 1225
DN GGVDCSD+EVNIKI LN +V GD+T KQRNQ+L SM DEV +V+ + Y Q++++S
Sbjct: 1159 DNVGGVDCSDNEVNIKIFLNGLVANGDLTLKQRNQILESMKDEVGSIVIEDAYGQSESIS 1218
Query: 1226 LAARRARERIAEYKRLMADLEARGKLDRAIEFLPSEEQLAERLAAGQGLTRAELSVLISY 1285
+ + + E R + +E G LDRA+E +P +E L ER G GLTR ELSVL++Y
Sbjct: 1219 VTEAQGVSLMKEQIRFIHHMEKAGYLDRALEHIPDDETLLERERQGMGLTRPELSVLMAY 1278
Query: 1286 SKIDLKEQLLKSLVPDDDYLTRDMETAFPPSLVSKFAEAMRRHRLKREIVSTQIANDLVN 1345
K+ LKE+L + D++ + + FP L +A+ M H L+ EI++T +AN +VN
Sbjct: 1279 GKMALKEELASEEIAQDEFHAKQLVNYFPTELRGHYAQQMVNHPLRVEIIATALANQMVN 1338
Query: 1346 NMGITFVQRLKESTGMSPANVAGAYVIVRDIFHLPHWFRQIEALDYQVPAEIQLTLMDEL 1405
MG FV RL+E TG S ++A AY R+I+ L ++ LD + Q +M +
Sbjct: 1339 EMGCNFVTRLQEETGSSVVDIANAYAAAREIYGLGIVLEKVRKLDNIAQSSAQYDVMFLV 1398
Query: 1406 MRLGRRATRWFLRSRRNEQDAGRDTAHFGPKIAQLGLKLDELLEGPTRERWMVRYQGFVD 1465
R RR TRW LR+R + + + + +LD++L + +++
Sbjct: 1399 RRTLRRLTRWLLRNRTGKPSVIAMVERYQEDVKAITEQLDKVLVKEEIVEHNSMAENWIE 1458
Query: 1466 AGVPELLARMVAGTSHLYTLLPIIEAADVTGHEPAQVAKAFFAVGSALDLTWYLQEISNL 1525
G+ + LA VA S LY++L I A G Q AK +F +G L L W+L++I++
Sbjct: 1459 KGIEKELAHYVARLSSLYSVLDISAVAKEKGIAVTQTAKLYFHLGDRLSLHWFLKQINHQ 1518
Query: 1526 PVENNWQALAREAFRDDIDLQQRAITISVL--QMADAPQDMDARVALWSEQHRGMVERWR 1583
V+N+WQALAR +FR+D+D QQR +T VL ++DA Q+++ + W E+++ + RW
Sbjct: 1519 AVDNHWQALARASFREDLDWQQRQLTAQVLSSNLSDAQQEIELALDKWLERNQVSISRWE 1578
Query: 1584 AMLDDLRNATGTDYAMYAVANRELVDLAMS 1613
+L + + T ++A ++VA REL L ++
Sbjct: 1579 NILSEFKVGTVHEFAKFSVALRELTLLNLN 1608