Pairwise Alignments

Query, 679 a.a., Methionine--tRNA ligase from Pseudomonas putida KT2440

Subject, 683 a.a., methionine--tRNA ligase from Pseudomonas fluorescens FW300-N2E3

 Score = 1245 bits (3222), Expect = 0.0
 Identities = 608/683 (89%), Positives = 643/683 (94%), Gaps = 4/683 (0%)

Query: 1   MSEPRQILVTSALPYANGSIHLGHMLEYIQTDMWVRFQKLRGNQCIYVCADDAHGSAIML 60
           MSEPR+ILVTSALPYANGSIHLGHMLEYIQTDMWVRFQK RGNQC YVCADDAHGSAIML
Sbjct: 1   MSEPRKILVTSALPYANGSIHLGHMLEYIQTDMWVRFQKHRGNQCTYVCADDAHGSAIML 60

Query: 61  RAEKEGITPEQLIANVQAEHSSDFADFLVDFDNFHSTHSDENRELSSLIYSRLREAGHIA 120
           RAEKEGITPEQLIANVQAEHS+DFA+FLVDFDNFHSTH++ENRELSS IY +LR+AGHIA
Sbjct: 61  RAEKEGITPEQLIANVQAEHSADFAEFLVDFDNFHSTHAEENRELSSQIYLKLRDAGHIA 120

Query: 121 TRSVTQYFDPEKGMFLADRFIKGTCPKCAAEDQYGDNCEKCGATYAPTELKNPKSAISGA 180
           TRS+TQYFDPEK MFLADRFIKGTCPKC  EDQYGDNCEKCGATYAPT+LK+PKSAISGA
Sbjct: 121 TRSITQYFDPEKKMFLADRFIKGTCPKCGTEDQYGDNCEKCGATYAPTDLKDPKSAISGA 180

Query: 181 TPVLRDSQHFFFKLPDFQAMLQQWTRSGTLQDAVANKLAEWLDSGLQEWDISRDAPYFGF 240
           TPVL+DSQHFFFKLPDFQ MLQ+WTRSGTLQDAVANK+AEWLD GLQ+WDISRDAPYFGF
Sbjct: 181 TPVLKDSQHFFFKLPDFQQMLQEWTRSGTLQDAVANKIAEWLDVGLQQWDISRDAPYFGF 240

Query: 241 EIPGEPGKYFYVWLDAPIGYMASFKNLCARRPELDFDAFWHEGSKAELYHFIGKDIVNFH 300
           EIP EPGKYFYVWLDAPIGYMASFKNLCARRPELDFDA+W + S AELYHFIGKDIVNFH
Sbjct: 241 EIPDEPGKYFYVWLDAPIGYMASFKNLCARRPELDFDAYWAKDSTAELYHFIGKDIVNFH 300

Query: 301 ALFWPAMLEGAGFRKPTAVNVHGYLTVNGAKMSKSRGTFIKARTYLDHLQPEYLRYYYAA 360
           ALFWPAMLEGAG+RKPT +NVHGYLTVNG KMSKSRGTFIKARTYLDHL PEYLRYYYA+
Sbjct: 301 ALFWPAMLEGAGYRKPTGINVHGYLTVNGQKMSKSRGTFIKARTYLDHLSPEYLRYYYAS 360

Query: 361 KLGRGVDDLDLNLEDFVQKVNSDLVGKVVNIASRCAGFIHKGNEGVMVGGDAAPELTEAF 420
           KLGRGVDDLDLNLEDFVQKVNSDLVGKVVNIASRCAGFIHKGN GV+V G+AAPELT+AF
Sbjct: 361 KLGRGVDDLDLNLEDFVQKVNSDLVGKVVNIASRCAGFIHKGNAGVLVAGNAAPELTDAF 420

Query: 421 LAAAPSIAEAYEARDFGRAMREIMALADRANAWIADKAPWSLAKQEGKQDEVQAICAQGI 480
           LAAAPSIAEAYEARDF RAMREIM LADRANAWIADKAPWSL KQEGKQDEVQAICA G+
Sbjct: 421 LAAAPSIAEAYEARDFARAMREIMGLADRANAWIADKAPWSLNKQEGKQDEVQAICALGV 480

Query: 481 NLFRQLVIFLKPVLPVLAADAEAFLNVAPLTWNDHLTRLENHQLNPFKALMSRIEPAKVE 540
           NLFRQLVIFLKPVLP+LAADAEAFLNVAPLTWNDH T L NHQLN FK LM+RI+P KV+
Sbjct: 481 NLFRQLVIFLKPVLPLLAADAEAFLNVAPLTWNDHATLLSNHQLNEFKPLMTRIDPVKVQ 540

Query: 541 AMVAASKEDLLAAEA----KAPAGNGELAKDPLSAEIEFDTFAAVDLRVALIVKAEAVAG 596
           AM  ASKEDL A++      AP GNGELAKDPLS EIEFDTFAAVDLRVALI+KAEAV G
Sbjct: 541 AMTDASKEDLAASQTDTGDAAPQGNGELAKDPLSPEIEFDTFAAVDLRVALILKAEAVEG 600

Query: 597 ADKLLQLTLDIGDERRNVFSGIKSAYPDPSKLEGRLTMMVANLKPRKMRFGVSEGMVMAA 656
           ADKLL+LTLDIGDE+RNVFSGIKSAYPDP+KLEGRLTMM+ANLKPRKMRFG+SEGMVMAA
Sbjct: 601 ADKLLRLTLDIGDEQRNVFSGIKSAYPDPAKLEGRLTMMIANLKPRKMRFGISEGMVMAA 660

Query: 657 GPGGEEIYLLSPDSGAKPGQRIK 679
           GPGGEEIYLLSPDSGAKPGQRIK
Sbjct: 661 GPGGEEIYLLSPDSGAKPGQRIK 683