Pairwise Alignments

Query, 1034 a.a., isoleucine--tRNA ligase from Methanococcus maripaludis S2

Subject, 937 a.a., isoleucine--tRNA ligase from Pectobacterium carotovorum WPP14

 Score =  379 bits (973), Expect = e-109
 Identities = 258/843 (30%), Positives = 421/843 (49%), Gaps = 97/843 (11%)

Query: 20  WKKENTYKKVKALNEHGPEYYFVDGPPYCSGAIHLGTAWNKIIKDTVLRFKRIQGYNVLD 79
           W +++ Y  ++   +    +   DGPPY +G+IH+G + NKI+KD +++ K + GY+   
Sbjct: 32  WYEQDLYGIIRNAKKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPY 91

Query: 80  KAGWDMHGLPIEVKVENEFNIGSKKDIETKIGTQEFINKCKEFALNHLGHMQGQFENLGV 139
             GWD HGLPIE+KVE    IG   +   K+   EF  +C+++A   +   +  F  LGV
Sbjct: 92  VPGWDCHGLPIELKVEQ--LIGKPGE---KVSAAEFRAECRKYAAEQVAGQKADFIRLGV 146

Query: 140 WLDFENAYMPIKRDYMEMGWWTLKKAHEKELLTKDLRSGYWCPRCETSLAEHEVRGEYKE 199
             D++  Y+ +           L +  E   L K  +  +WC  C ++LAE EV  EY +
Sbjct: 147 LGDWDRPYLTMDFKTEANIIRALGRIIENGHLHKGAKPVHWCADCGSALAEAEV--EYYD 204

Query: 200 VLDPSVYV------------KFKLEKSDEYIT--IWTTTPWTLPSNMLVCVNPEFDYAYV 245
              PS+ V            KF +   D  ++  IWTTTPWTLP+N  + +N EFDY  V
Sbjct: 205 KTSPSIDVAFNASDVAAVLAKFGVSSVDGPVSLVIWTTTPWTLPANRAISLNAEFDYQLV 264

Query: 246 NVEFENGTAETWIIAEKLVNDVMKKAEKNNDISKFSISKVVKGDSLIGLKYIHPLLEENE 305
            ++      +  I+A  LV  VMK+A     ++++ +    KG  L  L++ HP L  + 
Sbjct: 265 QID-----GQALILATDLVESVMKRA----GVAQWQVLGGCKGAELELLRFKHPFLGFDV 315

Query: 306 KQQEFAKIENVHTIVPGDHVTLEGGTGLVHTAPGFGEDDFNIGKEHNIPVYAPIDDNGKY 365
                         + GDHVTL+ GTG VHTA G G +D+ I +++N+ +  P+  NG Y
Sbjct: 316 ------------PAILGDHVTLDAGTGAVHTAGGHGPEDYVISQKYNLEIANPVGPNGCY 363

Query: 366 TDSIW---KGTFVKDMDESVIETLISKNLLVNSGKVKHTYPHCWRCKTPLLFRATEQWFL 422
               +    G FV   ++ ++E L  K +L++  K++H+YP CWR K+P++FRAT QWF+
Sbjct: 364 LSGTYPELDGKFVFKANDLIVEILREKGMLLHVEKLQHSYPCCWRHKSPIIFRATPQWFV 423

Query: 423 SISK--IKDSIIEQGKTVDWVPDWVKTRYVNGVSFVGDWNISRQRYWGIPLPIWICEECG 480
           S+ +  ++   + + K V W+PDW + R    V+   DW ISRQR WG+P+ +++ +E  
Sbjct: 424 SMDQKGLRKQSLSEIKGVQWIPDWGQARIEAMVANRPDWCISRQRTWGVPMSLFVHKETE 483

Query: 481 NY-----EVIGSVDELKERANEK---DVDLSDIHKPAVDKITLTCSCGGKMKRTPDVLDV 532
                  E+I +V +  E    +   D+D +D+     D             + PD LDV
Sbjct: 484 ELHPRTAELIEAVAKRVEADGIQAWWDLDPADVLGADADNYV----------KVPDTLDV 533

Query: 533 WYDSGLAPYASIGSKKLK----KAQFITEGNDQVTKWFYSQHALSAVVFDDTSYEKCMMH 588
           W+DSG + ++S+   + +    +A    EG+DQ   WF S   +S  +     Y + + H
Sbjct: 534 WFDSG-STHSSVVDVRPEFSGHEADMYLEGSDQHRGWFMSSLMISTAIKGKAPYRQVLTH 592

Query: 589 GFTLDETGEKMSKSLGNIVSPDDVTEQYGADVLRFYLLSANKAWEDLRFSYSEMDETRSM 648
           GFT+D  G KMSKS+GN VSP DV  + GAD+LR ++ S + + E      +  DE    
Sbjct: 593 GFTVDGQGRKMSKSIGNTVSPQDVMNKLGADILRLWIGSTDYSGE-----IAVSDEILKR 647

Query: 649 LNTLWNSYAFSANYMV--LDDFVPNNEYFKHVKDE-----DAWILSRINTVAKEAVEALE 701
               +     +A +++  L+ F P  +    VK E     D W +S      +E +EA E
Sbjct: 648 SADAYRRIRNTARFLLANLNGFDPQKD---SVKPEDMVVLDRWAVSCAKAAQEEILEAYE 704

Query: 702 KPHLHVYTWALRDFILNDFSRWYIKLIRDRTWMEKND-VQKLSAYQTLYYVIMKLISIMA 760
               H     L  F   +   +Y+ +I+DR +  K+D V + S    LY++   L+  MA
Sbjct: 705 SYDFHRVVQRLMQFCSIEMGSFYLDIIKDRQYTAKSDSVARRSCQTALYHISEALVRWMA 764

Query: 761 PVTPHLSEEIY--------QNLKTEDMPESIFMNKLTIESEFINETLEKDTEIIREIVDS 812
           P+    ++EI+        Q + TE+  + +F       +E +N+    D   +R  V+ 
Sbjct: 765 PIMSFTADEIWNYLPGERAQYVFTEEWYDGLF---ALDSAETMNDAFWADVLKVRSEVNK 821

Query: 813 ILK 815
           +++
Sbjct: 822 VIE 824



 Score = 27.7 bits (60), Expect = 0.004
 Identities = 18/60 (30%), Positives = 29/60 (48%), Gaps = 3/60 (5%)

Query: 520 GGKMKRTPDVLDVWYDSGLAPYASI-GSKKLKKAQFITEGNDQVTKWFYSQHALSAVVFD 578
           G   KR PD+L  WY+  L  Y  I  +KK KK   + +G        +  H+++ ++ D
Sbjct: 19  GDLAKREPDMLKRWYEQDL--YGIIRNAKKGKKTFILHDGPPYANGSIHIGHSVNKILKD 76