Pairwise Alignments

Query, 1034 a.a., isoleucine--tRNA ligase from Methanococcus maripaludis S2

Subject, 941 a.a., isoleucine--tRNA ligase from Dickeya dianthicola ME23

 Score =  371 bits (952), Expect = e-106
 Identities = 250/839 (29%), Positives = 415/839 (49%), Gaps = 85/839 (10%)

Query: 20  WKKENTYKKVKALNEHGPEYYFVDGPPYCSGAIHLGTAWNKIIKDTVLRFKRIQGYNVLD 79
           W +++ Y  ++   +    +   DGPPY +G IH+G + NKI+KD +++ K + GY+   
Sbjct: 32  WYEQDLYGIIRGAKKGKKTFILHDGPPYANGNIHIGHSVNKILKDIIIKSKGLSGYDSPY 91

Query: 80  KAGWDMHGLPIEVKVENEFNIGSKKDIETKIGTQEFINKCKEFALNHLGHMQGQFENLGV 139
             GWD HGLPIE+KVE    +G   +   K+   +F  +C+++A   +   +  F  LGV
Sbjct: 92  VPGWDCHGLPIELKVEQL--VGKPGE---KVSAAQFREECRKYAAEQVAGQKKDFIRLGV 146

Query: 140 WLDFENAYMPIKRDYMEMGWWTLKKAHEKELLTKDLRSGYWCPRCETSLAEHEVRGEYKE 199
             +++  Y+ +           L +  E   L K  +  +WC  C ++LAE EV  EY +
Sbjct: 147 LGEWDRPYLTMDFKTEANIIRALGRIIENGHLHKGAKPVHWCADCGSALAEAEV--EYYD 204

Query: 200 VLDPSVYVKFK-------LEK---------SDEYITIWTTTPWTLPSNMLVCVNPEFDYA 243
              P++ V F        L K          +  + IWTTTPWTLP+N  + +N EFDY 
Sbjct: 205 KTSPAIDVAFNAVDRAAVLAKFGLAADAVDGEVALVIWTTTPWTLPANRAISLNAEFDYV 264

Query: 244 YVNVEFENGTAETWIIAEKLVNDVMKKAEKNNDISKFSISKVVKGDSLIGLKYIHPLLEE 303
            + V       +  I+AE L + V K+   ++ +++  +   VKG  L  L++ HP ++ 
Sbjct: 265 LIQV-----AGKAVIVAEGLADAVAKRVTGSDAVTR--LGHAVKGSELELLRFRHPFMDF 317

Query: 304 NEKQQEFAKIENVHTIVPGDHVTLEGGTGLVHTAPGFGEDDFNIGKEHNIPVYAPIDDNG 363
           +               + GDHVTL+ GTG VHTA G G DD+ I +++ + +  P+  NG
Sbjct: 318 DVPA------------ILGDHVTLDAGTGAVHTAGGHGPDDYVISQKYQLEIANPVGPNG 365

Query: 364 KYTDSIWKGT---FVKDMDESVIETLISKNLLVNSGKVKHTYPHCWRCKTPLLFRATEQW 420
            Y      G    FV   ++ ++E L  +  L++  K++H+YP CWR K+P+LFRAT QW
Sbjct: 366 CYLPGTLPGLDGLFVFKANDKIVELLRERGALLHHEKLQHSYPCCWRHKSPILFRATPQW 425

Query: 421 FLSISK--IKDSIIEQGKTVDWVPDWVKTRYVNGVSFVGDWNISRQRYWGIPLPIWICEE 478
           F+S+ +  ++   + + K V W+PDW + R  + V+   DW ISRQR WG+P+ +++ +E
Sbjct: 426 FVSMDQNGLRKQSLSEIKGVQWIPDWGQARIESMVANRPDWCISRQRTWGVPMSLFVHKE 485

Query: 479 CG--NYEVIGSVDELKERANEKDVDLSDIHKPAVDKITLTCSCGGKMKRTPDVLDVWYDS 536
               +      +D + +   +  +       PA     L  +     ++ PD LDVW+DS
Sbjct: 486 TQALHPRTAELIDAVAKHVEQDGIQAWWDLNPA----DLLGADAADYEKVPDTLDVWFDS 541

Query: 537 GLAPYASIGSKKLK----KAQFITEGNDQVTKWFYSQHALSAVVFDDTSYEKCMMHGFTL 592
           G + +AS+   + +    +A    EG+DQ   WF S   +S  +     Y + + HGFT+
Sbjct: 542 G-STHASVVDVRPEFSGHEADMYLEGSDQHRGWFMSSLMISTAIKGKAPYRQVLTHGFTV 600

Query: 593 DETGEKMSKSLGNIVSPDDVTEQYGADVLRFYLLSANKAWEDLRFSYSEMDETRSMLNTL 652
           D  G KMSKS+GN VSP DV ++ GAD+LR ++ S + + E      +  DE        
Sbjct: 601 DGQGRKMSKSIGNTVSPQDVMDKLGADILRLWIGSTDYSGE-----IAVSDEILKRSADA 655

Query: 653 WNSYAFSANYMV--LDDFVPNNEYFKHVKDE-----DAWILSRINTVAKEAVEALEKPHL 705
           +     +A +++  L+ F P       VK E     D W +S       E VEA E    
Sbjct: 656 YRRIRNTARFLLANLNGFDPAQH---SVKPEDMVVLDRWAVSCAKAAQDEIVEAYESYDF 712

Query: 706 HVYTWALRDFILNDFSRWYIKLIRDRTWMEKND-VQKLSAYQTLYYVIMKLISIMAPVTP 764
           H     L  F   +   +Y+ +I+DR +  K+D V + S    L+++   L+  MAP+  
Sbjct: 713 HRVVQRLMQFCSIEMGSFYLDIIKDRQYTAKHDSVARRSCQTALFHIAEALVRWMAPILS 772

Query: 765 HLSEEIY--------QNLKTEDMPESIFMNKLTIESEFINETLEKDTEIIREIVDSILK 815
             ++EI+        Q + TE+  + +F      +SE +N+    D   +R  V+ +++
Sbjct: 773 FTADEIWAYLPGERAQFVFTEEWYDGLFG---LADSEPMNDAFWADMLNVRGEVNKVIE 828



 Score = 29.6 bits (65), Expect = 0.001
 Identities = 19/60 (31%), Positives = 30/60 (50%), Gaps = 3/60 (5%)

Query: 520 GGKMKRTPDVLDVWYDSGLAPYASI-GSKKLKKAQFITEGNDQVTKWFYSQHALSAVVFD 578
           G   KR PD+L  WY+  L  Y  I G+KK KK   + +G        +  H+++ ++ D
Sbjct: 19  GDLAKREPDMLKRWYEQDL--YGIIRGAKKGKKTFILHDGPPYANGNIHIGHSVNKILKD 76