Pairwise Alignments
Query, 614 a.a., AMP-binding protein (NCBI ptt file) from Shewanella oneidensis MR-1
Subject, 3200 a.a., non-ribosomal peptide synthetase from Paraburkholderia sabiae LMG 24235
Score = 82.0 bits (201), Expect = 4e-19
Identities = 136/589 (23%), Positives = 230/589 (39%), Gaps = 100/589 (16%)
Query: 38 HLKLA--AHHIPHHLAVAVQQGKGKSFANLTYQELDFISLNKQSDAIAFALNAYGLTRGM 95
HL+L A PH A+A +F+ LT + + + L+ ++ +A AL A+G +
Sbjct: 9 HLRLRELARSAPHAPALA-------AFSPLTVR-MSRVELDARASRVAAALRAHGAGAEV 60
Query: 96 KAVLMVTPSLDFFALTFALFKAGIIPVLVDPGMGIKNLKQCFIEAAPDAFIGIPKAHIAR 155
+ + + S D F A+ KAG + V +DP + L +A G+ + R
Sbjct: 61 RVGVCIDRSCDLFVALLAVLKAGGVFVPLDPRHPAERLDWMTRDA------GLRHGIVTR 114
Query: 156 RLLGWGKASVKRLINVDANQSGVTDTLSRLLTGAPSLASMLSFTTKSSSAKLPEQVEYPM 215
+A + I+VDA + + VE+
Sbjct: 115 DASASLRARFEHCIDVDA-------------------------------IDIEQPVEFED 143
Query: 216 ALLEHDEMAAILFTSGSTGTPKGVVYSHGMFEAQIQALKQDYGIAHGERDL----ATFPL 271
AL+ A +++TSGSTGTPK VV HG A A+ Y + +R L F L
Sbjct: 144 ALVHPRSAAYMIYTSGSTGTPKAVVVEHGPLAAHCDAVIGAYPMTDADRVLHFASVNFDL 203
Query: 272 FSLF--GPALGMTSIVPEMDASKPITANPEFLFAAIEKYQCSNIFVNPALLERLGRAGEQ 329
+ P SI + P T P+ A I + + PA L +A
Sbjct: 204 AHEYWLAPLAAGASIA----ITAPGTVAPDDARALIHHERVTIAAFPPAYLREFAQAA-- 257
Query: 330 TDSKNQHKLSSVKRVISAGAPATIASIARFSKMLSDGVPVLNSYGATESLPISMIASDEL 389
+H + RV++ G A + + + V ++N YG TE++ M+ +
Sbjct: 258 ----RRHGVPDSLRVLAFGGEAMPGDVFGEIRQTFNNVRLINGYGPTETVISPMLWPLDP 313
Query: 390 FTTTQVTDNGGGICVGRAIDG-VKIEIIAITEADIPEWDNRLCLNAGEIGEIVVTGQMVS 448
+ T C I + + + +TEA D L + G GE+++ G ++
Sbjct: 314 RAAYEDTS-----CASLPIGAPIGLRVARMTEAG--SVDGAL-IEHGACGELLLGGACLA 365
Query: 449 QSYYHREKATAASKIWDS--ERQTFRHRMGDLGYLDDSGRLWMCGRKAHRVD----ATQG 502
+ Y+ R TA I D+ E + +R GDL L G GR +V +
Sbjct: 366 RGYHGRAAQTAERFIPDANGEPGSRVYRTGDLARLRTDGAYDYMGRIDDQVQIRGVRVEP 425
Query: 503 GQFAKRYYSIPCERIFNTHPNVKRSALVGVTVKGQHGVGEIKPLICIELDQSLVCNKSAQ 562
G+ A+ +HP+V+ +A V +H G ++ C+ + ++L + +
Sbjct: 426 GEIAQS---------LRSHPDVRDAA-----VLVEHIAGRVQLTACVVVTEAL---EESA 468
Query: 563 LYQELMVIAEQYSQTQGIRRFLIHPDFPVDVRHNAKIFREKLAVWAQSQ 611
L + L ++ RF + P + N K+ RE L SQ
Sbjct: 469 LREHL---TQRLPDAWQPHRFALLDALPYTL--NGKLDREALRARVASQ 512
Score = 75.1 bits (183), Expect = 4e-17
Identities = 103/435 (23%), Positives = 169/435 (38%), Gaps = 75/435 (17%)
Query: 68 QELDFISLNKQSDAIAFALNAYGLTRGMKAVLMVTPSLDFFALTFALFKAGIIPVLVDPG 127
++L F +LN+ S A AL G L + S+DF A+ K+G + V +DP
Sbjct: 1096 RQLTFEALNEASSRAARALLEKGAGAEQAIALCIERSVDFVVALLAVLKSGSMAVPLDP- 1154
Query: 128 MGIKNLKQCFIEAAPDAFIGIPKAHIARRLLGWGKASVKRLINVDANQSGV-TDTLSRLL 186
AAP RL +A R + V +N V T+ LS
Sbjct: 1155 ------------AAP-----------RERLEASIRACGARCVIVASNALQVETEVLS--- 1188
Query: 187 TGAPSLASMLSFTTKSSSAKLPEQVEYPMALLEHDEMAAILFTSGSTGTPKGVVYSHGMF 246
L+ +++S P + D+ A +++TSGSTGTPKGVV SH
Sbjct: 1189 ---------LNALIEANSHDAP-------VTITPDQAAYLIYTSGSTGTPKGVVVSHRAL 1232
Query: 247 EAQIQALKQDYGIAHGERDLATFPLFSLFGPALGMTSIVPEMDAS-------KPITANPE 299
+Q + + A A+ + S LG T++ + + K +P+
Sbjct: 1233 ADYVQGMLDELKFAPD----ASMAMVSTVAADLGHTTLFGALCSGRTLHLLPKECAFDPD 1288
Query: 300 FLFAAIEKYQCSNIFVNPALLERLGRAGEQTDSKNQHKLSSVKRVISAGAPATIASIARF 359
+ + + + P+ L L A + T H L + G P + R
Sbjct: 1289 RFAVTMRERGVGVLKIVPSHLHALLEAQQPTAVLPSHAL------VLGGEPLPWTLVERI 1342
Query: 360 SKMLSDGVPVLNSYGATESLPISMIASDELFTTTQVTDNGGGICVGRAIDGVKIEIIAIT 419
+ L V+N YG TE+ + + D + + G+ GR + ++ ++ +
Sbjct: 1343 TG-LRPACRVINHYGPTEA-TVGALTFDAT-ASLSAANASTGVPTGRPLPNARVIVLDVH 1399
Query: 420 EADIPEWDNRLCLNAGEIGEIVVTGQMVSQSYYHREKATAASKIWDSE--RQTFRHRMGD 477
A +P G IGE+ + G V++ Y HR ATA + D+ + +R GD
Sbjct: 1400 GAPVP---------VGGIGELYLGGPGVARGYLHRAAATAERFVPDASGPKGARMYRTGD 1450
Query: 478 LGYLDDSGRLWMCGR 492
L G + GR
Sbjct: 1451 RVRLRADGAIDYLGR 1465
Score = 70.5 bits (171), Expect = 1e-15
Identities = 103/429 (24%), Positives = 170/429 (39%), Gaps = 69/429 (16%)
Query: 72 FISLNKQSDAIAFALNAYGLTRGMKAVLMVTPSLDFFALTFALFKAGIIPVLVDPGMGIK 131
+ LN++++ +A AL G++ L V S+D F + KAG + VDP +
Sbjct: 2620 YAELNERANRVAGALLNAGVSADSVVALCVERSIDMVVALFGVLKAGAAYLPVDPDYPAE 2679
Query: 132 NLKQCFIEAAPDAFIGIPKAHIARRLLGWGKASVKRLINVDANQSGVTDTLSRLLTGAPS 191
+ +A P A + +A AR + G+A L LT P+
Sbjct: 2680 RIAYLLDDARP-AVVLTQRALHARVVDSIGRADACVLC-------------VEQLTSNPA 2725
Query: 192 LASMLSFTTKSSSAKLPEQVEYPMALLEHDEMAAILFTSGSTGTPKGVVYSHGMFEAQIQ 251
L S + +E D++A +++TSGSTG PKG +H +I
Sbjct: 2726 LESSID--------------------VEPDQLAYLIYTSGSTGKPKGAGNTHRALANRIA 2765
Query: 252 ALKQDYGIAHGERDLATFPLFSLFGPALGMTSIVPEMDASKPIT-------ANPEFLFAA 304
++ Y + + L P FG + + V + + +P L AA
Sbjct: 2766 WMQGAYSLTPRDVVLHKTP----FGFDVSVWEFVWPLSVGATLAIAAPGDHRDPARLAAA 2821
Query: 305 IEKYQCSNIFVNPALLERLGRAGEQTDSKNQHKLSSVKRVISAGAPATIASIARFSKMLS 364
I Y + + P++L G D + S+ RV+++G + +AR +++L
Sbjct: 2822 IAAYGVTTLHFVPSMLGAF--LGHLKDFDAAAQCESIVRVVASGEALSPELVAR-ARLLL 2878
Query: 365 DGVPVLNSYGATESLPISMIASDELFTTTQVTDNGGGICVGRAIDGVKIEIIAITEADIP 424
+ N YG TE A+ ++ T D+ + V I++ A+ A P
Sbjct: 2879 PQAQLHNLYGPTE-------AAIDVSHWTCTDDDAQAVAVPIGEPIANIQLHALDAALHP 2931
Query: 425 EWDNRLCLNAGEIGEIVVTGQMVSQSYYHREKATAASKIWDSERQTFR-HRMGDL----- 478
L AG IGE+ + G +++ Y R TA + D R +R GDL
Sbjct: 2932 -------LPAGAIGELYLGGVGLARGYLGRAALTAERFVPDPFTPGARLYRTGDLVCRRA 2984
Query: 479 -GYLDDSGR 486
G LD GR
Sbjct: 2985 DGVLDYLGR 2993