Pairwise Alignments

Query, 474 a.a., tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB from Escherichia coli ECOR38

Subject, 442 a.a., (dimethylallyl)adenosine tRNA methylthiotransferase from Pseudomonas fluorescens FW300-N2C3

 Score =  592 bits (1526), Expect = e-174
 Identities = 289/442 (65%), Positives = 352/442 (79%), Gaps = 1/442 (0%)

Query: 1   MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLLNTCSIREKAQEKVF 60
           M KKL+I+T GCQMNEYDSS+M DLL      ++T  AE+ADV+LLNTCSIRE+AQ++V+
Sbjct: 1   MAKKLYIETHGCQMNEYDSSRMVDLLGEHQALEVTARAEDADVILLNTCSIRERAQDRVY 60

Query: 61  HQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRG 120
            QLGRW+ LK  NP+++I VGGCVASQEG  IR RA YVD++FGPQTLHRLPEMI++ R 
Sbjct: 61  SQLGRWRELKLANPEMVIAVGGCVASQEGAAIRDRAPYVDVVFGPQTLHRLPEMIDAARA 120

Query: 121 DRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYCTYCVVPYTRGEEVSRPSDD 180
            + P VD+SFPEIEKFD LPEPR +GP+A+VS+MEGC+KYCT+CVVPYTRGEEVSRP DD
Sbjct: 121 TKLPQVDVSFPEIEKFDHLPEPRIDGPSAYVSVMEGCSKYCTFCVVPYTRGEEVSRPFDD 180

Query: 181 ILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSFADLLRLVAAIDGIDRIRFTTSH 240
           ++ EI  LA  GVREV LLGQNVN +RG  +DG     A+L+R+VAA+DGIDRIR+TTSH
Sbjct: 181 VIAEIIHLAENGVREVTLLGQNVNGYRGLTHDGRLADLAELIRVVAAVDGIDRIRYTTSH 240

Query: 241 PIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNLMGRTHTALEYKAIIRKLRAARPDI 300
           P+EF+D +I+ + + PELV  LHLPVQSGSDRIL  M R HTALEYK+ +RKLRAA P I
Sbjct: 241 PLEFSDSLIQAHAEVPELVKHLHLPVQSGSDRILAAMKRNHTALEYKSKLRKLRAAVPGI 300

Query: 301 QISSDFIVGFPGETTEDFEKTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQ 360
            ISSDFIVGFPGET +DF++TMKLI DV FD SYSF++S RPGTPAAD+ DD PEE KK+
Sbjct: 301 CISSDFIVGFPGETEKDFQQTMKLIEDVGFDFSYSFVYSQRPGTPAADLADDTPEELKKE 360

Query: 361 RLYILQERINQQAMAWSRRMLGTTQRILVEGTSRKSIMELSGRTENNRVVNFE-GTPDMI 419
           RL  LQ R+NQQ    SR+M+G+ QRILV   S+K   EL GRTENNR+VNF   TP +I
Sbjct: 361 RLNALQHRLNQQGFEISRQMVGSVQRILVTDYSKKDPGELQGRTENNRIVNFRCDTPALI 420

Query: 420 GKFVDVEITDVYPNSLRGKVVR 441
           G+F DV I    P+SLRG +++
Sbjct: 421 GQFADVHIDAAQPHSLRGSLIQ 442