Pairwise Alignments
Query, 1517 a.a., glutamate synthase, large subunit from Escherichia coli BL21
Subject, 1567 a.a., glutamate synthase from Burkholderia phytofirmans PsJN
Score = 1201 bits (3106), Expect = 0.0
Identities = 687/1557 (44%), Positives = 924/1557 (59%), Gaps = 80/1557 (5%)
Query: 33 LYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGAILADGKTGDGCGLLLQK 92
LYD E D CG G +AHI+G+ SH++++ + L + HRGA+ AD GDG G+L+Q
Sbjct: 17 LYDPQNEHDACGVGFVAHIKGKKSHEIIQQGLKILENLDHRGAVGADPLMGDGAGILIQI 76
Query: 93 PDRFFRIVAQERGWRLAKN--YAVGMLFLNKDPELAAAARRIVEEELQRETLSIVGWRDV 150
PD F+R ++G L + Y VGM+FL K+ A + +E ++ E ++GWRDV
Sbjct: 77 PDGFYREEMAKQGVVLPPHGEYGVGMVFLPKEHASRLACEQELERTVKAEGQVVLGWRDV 136
Query: 151 PTNEGV-LGEIALSSLPRIEQIFVNAPAGWRPRD-MERRLFIARRRIEKRLEA-----DK 203
P + + + +S P I QIF+ D +ER+L+I R+ R++A K
Sbjct: 137 PVDHTMPISPTVKASEPLIRQIFIGRGKDIMVTDALERKLYIIRKTASHRIQALKLKHGK 196
Query: 204 DFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRFSTNTVPRWPLAQPF 263
+++V S S +YKGL + + +Y DL D R SA+ L HQRFSTNT P W LA P+
Sbjct: 197 EYFVPSCSARTVVYKGLLLAGQVGVYYRDLQDDRTVSALALVHQRFSTNTFPAWELAHPY 256
Query: 264 RYLAHNGEINTITGNRQWARARTYKFQTPLI-PDLHDAAPFVNETGSDSSSMDNMLELLL 322
R +AHNGEINT+ GN W ART + ++ DL P + SD++S DN LELL+
Sbjct: 257 RMIAHNGEINTVKGNVNWLNARTGAIASHVLGDDLPKLWPLIYPGQSDTASFDNCLELLV 316
Query: 323 AGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWDGPAGIVMSDGRFAACNL 382
G ++ AM +++P AW+ + MD RAF+++++ MEPWDGPA I +DGR L
Sbjct: 317 MAGYPLVHAMMMMIPEAWEQHTLMDDNRRAFYEYHAAMMEPWDGPAAIAFTDGRQIGATL 376
Query: 383 DRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVGPGELMVIDTRSGRILHSAE 442
DRNGLRPARY++T D L+ ASE G ++V+K R+ PG++ +ID GRI+ E
Sbjct: 377 DRNGLRPARYIVTDDDLVIMASEAGTLPIPESKIVKKWRLQPGKMFLIDMEHGRIIDDKE 436
Query: 443 TDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELDDDTLASYQKQFNYSAEELDSV 502
D+L + PYK W++ VR + + E+V + + L Q+ F Y+ E+L +
Sbjct: 437 LKDNLANAKPYKSWIDA-VRIKLDEIEAKAEDVATERREAAALLDRQQAFGYTQEDLKFL 495
Query: 503 IRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQQFAQVTNPPIDPLREAHVMSLAT 562
+ + + G+EAVGSMG+D+P AV+S++ + +Y YF+Q FAQVTNPPIDP+RE VMSL +
Sbjct: 496 MAPMAQAGEEAVGSMGNDSPLAVMSNKNKTLYHYFKQLFAQVTNPPIDPIRENMVMSLVS 555
Query: 563 SIGREMNVF-CEAEGQAHRLSFKSPILLYSDFKQLTTMKE---EHYRADTLDITFDVT-- 616
+G + N+ RL P+L + D ++ + + + + L+I + V
Sbjct: 556 FVGPKPNLLDTNNINPPMRLEVSQPVLDFKDIAKIRAIDQYTGGKFSSYELNICYPVAWG 615
Query: 617 KTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLPVPAPMAVGAIQTRLVDQSLRC 676
K +EA + LC +A V+SG +L++SDR +D + +PA +A AI T LV LR
Sbjct: 616 KEGIEARLASLCAEAVDAVKSGYNMLIVSDRKTDRDNVAIPALLATAAIHTHLVQHGLRT 675
Query: 677 DANIIVETASARDPHHFAVLLGFGATAIYPYLAYETLGRLVDTHAIAKDYRTVMLNYRNG 736
A ++VET SAR+ HHFA+L G+GA A++PYLA ETL +L + N+
Sbjct: 676 SAGLVVETGSARETHHFALLAGYGAEAVHPYLAMETLSQLASGLKGDLSAEKAVYNFTKA 735
Query: 737 INKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDVVGLCFQGAVSRIGGASFEDFQQDLL 796
+ KGL K+MSKMGIST SY +++FEAVGL +D+V F+G S++GG D ++ +
Sbjct: 736 VGKGLMKVMSKMGISTYMSYTGAQIFEAVGLAEDLVVKYFKGTSSKVGGIGLFDVAEEAI 795
Query: 797 NLSKRAW----LARKPISQGGLLKYVHGGEYHAYNPDVVRTLQQAVQSGEYSDYQEYAKL 852
L + A+ + + GG Y GE H + PD + LQ + +S Y Y+EYA L
Sbjct: 796 RLHRDAFGDNPVLANMLDAGGEYAYRVRGEEHMWTPDAIAKLQHSARSNSYQTYKEYAHL 855
Query: 853 VNERPA--TTLRDLLAIT-PGENAVNIADVEPASELFKRFDTAAMSIGALSPEAHEALAE 909
+N++ T R L A+ + +VE A E+ KRF T AMS+G++S EAH LA
Sbjct: 856 INDQTKRHMTFRGLFEFKFDPTKAIPLDEVESAKEIVKRFATGAMSLGSISTEAHATLAV 915
Query: 910 AMNSIGGNSNSGEGGEDPARY-------------------------------GTNKVSRI 938
AMN IGG SN+GEGGED RY G + S+I
Sbjct: 916 AMNRIGGKSNTGEGGEDVNRYRNELRGIPIKNGDTMKSVIGDEVIVDIPLKEGDSLRSKI 975
Query: 939 KQVASGRFGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLIS 998
KQVASGRFGVT YL +AD IQIK+AQGAKPGEGGQLPG KV+ YI KLRYSVPGV LIS
Sbjct: 976 KQVASGRFGVTAEYLASADQIQIKMAQGAKPGEGGQLPGHKVSDYIGKLRYSVPGVGLIS 1035
Query: 999 PPPHHDIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYD 1058
PPPHHDIYSIEDLAQLI DLK N A ISVKLVSE GVGT+A GVAKA AD + IAG+D
Sbjct: 1036 PPPHHDIYSIEDLAQLIHDLKNANSAASISVKLVSESGVGTVAAGVAKAKADHVVIAGHD 1095
Query: 1059 GGTGASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGA 1118
GGTGASPLSSVK+AG PWELGL ETQQ LV N LR +IR+Q DG +KTG D++ A+LGA
Sbjct: 1096 GGTGASPLSSVKHAGTPWELGLAETQQTLVLNQLRGRIRVQADGQMKTGRDVVIGALLGA 1155
Query: 1119 ESFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARET 1178
+ FGF T P+V GC +R CHLN C GVATQD LR + G P V N+F F+A E
Sbjct: 1156 DEFGFATAPLVVEGCIMMRKCHLNTCPVGVATQDPVLRAK-FQGQPEHVVNFFFFVAEEA 1214
Query: 1179 RELMAQLGVTRLVDLIGRTDLLKELDGFTA-KQQKLALSKLLETAEPHPGKALYCTENNP 1237
RE+MAQLG+ + DLIG +LL G K + L S++ + A ++
Sbjct: 1215 REIMAQLGIRKFDDLIGHAELLDMKKGIEHWKAKGLDFSRVFYLPQV---SAEVARKHVD 1271
Query: 1238 PFDNGL---LNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLAA 1294
D+GL L+ L+++AK +++ + +F +RN +R+VGA LSG IA+ +G GL
Sbjct: 1272 VQDHGLDKALDHTLIEKAKAAIEKGEHVSFIQPVRNVNRTVGAMLSGTIAKKYGHDGLPD 1331
Query: 1295 DPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSHEAS 1354
D I GTAGQSFG + A GV L L GD NDYVGKG++GG I IRP +S E
Sbjct: 1332 DAIHIQLKGTAGQSFGAFLAKGVTLDLVGDGNDYVGKGLSGGRIIIRPTNDFRGKSEENI 1391
Query: 1355 IIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCILGKTGV 1414
I GNT +YGA G + G AGERF VRNSGA VVEG GD+GCEYMTGG V +LG+TG
Sbjct: 1392 ICGNTVMYGAIEGEAFFRGVAGERFCVRNSGATAVVEGTGDHGCEYMTGGTVVVLGETGR 1451
Query: 1415 NFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSV---------------DALAIHEEHLR 1459
NF AGM+GG AYV D F + N +V + V A A E L+
Sbjct: 1452 NFAAGMSGGIAYVYDPDSSFAGKCNKSMVALDPVLQTAEQERTVDKGLWHAGATDEALLK 1511
Query: 1460 GLITEHVQHTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQA 1516
GLI H Q TGS R + +L NW +F V P ++ K LG + A V A
Sbjct: 1512 GLIERHFQFTGSPRAKALLENWDASRRQFVKVFP--TEYKRALGEMAAKKANKEVLA 1566