SEED Subsystem: Serine-glyoxylate cycle in Pseudomonas syringae pv. syringae B728a

Experiments:

Role Gene Name  
3-ketoacyl-CoA thiolase (EC 2.3.1.16) Psyr_2725
5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20) Psyr_0459
5-FCL-like protein, but predicted not to be 5-formyltetrahydrofolate cyclo-ligase (5-FCL) No genes
5-formyltetrahydrofolate cyclo-ligase (EC 6.3.3.2) Psyr_0317
Acetoacetyl-CoA reductase (EC 1.1.1.36) No genes
Acetyl-CoA acetyltransferase (EC 2.3.1.9) No genes
Aconitate hydratase (EC 4.2.1.3) Psyr_3404
Citrate synthase (EC 2.3.3.1) No genes
Citrate synthase (si) (EC 2.3.3.1) Psyr_2004
Enolase (EC 4.2.1.11) Psyr_1363
  Psyr_1482
Formate--tetrahydrofolate ligase (EC 6.3.4.3) No genes
Formiminotetrahydrofolate cyclodeaminase (EC 4.3.1.4) No genes
Fumarate hydratase class I, aerobic (EC 4.2.1.2) Psyr_4031
Fumarate hydratase class I, anaerobic (EC 4.2.1.2) No genes
GDSL-like lipase/acylhydrolase No genes
Glycerate kinase (EC 2.7.1.31) Psyr_1490
  Psyr_1616
  Psyr_3115
  Psyr_3725
  Psyr_4233
  Psyr_4774
Isocitrate lyase (EC 4.1.3.1) Psyr_3196
Malate dehydrogenase (EC 1.1.1.37) No genes
Malate synthase-related protein No genes
Malyl-CoA lyase (EC 4.1.3.24) No genes
Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9) Psyr_1743
  Psyr_2225
Methylcrotonyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.4) Psyr_2467
Methylcrotonyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.4) Psyr_1538
  Psyr_2469
Methylene tetrahydromethanopterin dehydrogenase (EC 1.5.99.9) No genes
Methylenetetrahydrofolate dehydrogenase (NADP+) (EC 1.5.1.5) Psyr_1743
  Psyr_2225
Methylmalonyl-CoA mutase (EC 5.4.99.2) No genes
N(5),N(10)-methenyltetrahydromethanopterin cyclohydrolase (EC 3.5.4.27) No genes
Phosphoenolpyruvate carboxykinase [ATP] (EC 4.1.1.49) Psyr_0168
Phosphoglyceromutase No genes
Propionyl-CoA carboxylase beta chain (EC 6.4.1.3) No genes
Propionyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.3) No genes
Putative malate dehydrogenase (EC 1.1.1.37), similar to archaeal MJ1425 No genes
Serine hydroxymethyltransferase (EC 2.1.2.1) Psyr_4270
  Psyr_4712
Serine--glyoxylate aminotransferase (EC 2.6.1.45) No genes
Serine--pyruvate aminotransferase (EC 2.6.1.51) No genes
Serine-pyruvate aminotransferase/archaeal aspartate aminotransferase No genes
Succinate dehydrogenase flavoprotein subunit (EC 1.3.99.1) Psyr_2007
Succinate dehydrogenase iron-sulfur protein (EC 1.3.99.1) Psyr_2008
Succinyl-CoA ligase [ADP-forming] alpha chain (EC 6.2.1.5) Psyr_2013
Succinyl-CoA ligase [ADP-forming] beta chain (EC 6.2.1.5) Psyr_2012
Succinyl-CoA:3-ketoacid-coenzyme A transferase subunit A (EC 2.8.3.5) Psyr_3237
Succinyl-CoA:3-ketoacid-coenzyme A transferase subunit B (EC 2.8.3.5) Psyr_3238
crotonyl-CoA reductase No genes
cytosolic long-chain acyl-CoA thioester hydrolase family protein Psyr_0461
  Psyr_5043
  Psyr_5069
decarboxylase No genes
isobutyryl-CoA dehydrogenase No genes
low-specificity D-threonine aldolase Psyr_3398
polyhydroxyalkanoate synthesis repressor PhaR No genes
putative glycerate kinase No genes
putative isobutyryl-CoA mutase, chain B No genes

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