SEED Subsystem: Serine-glyoxylate cycle in Sinorhizobium meliloti 1021

Experiments:

Role Gene Name L-Lysine (C)  
3-ketoacyl-CoA thiolase (EC 2.3.1.16) SMc00262 -0.5
5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20) SMa2143 +0.1
  SMc01843 -7.2
  SMc04340 +0.2
5-FCL-like protein, but predicted not to be 5-formyltetrahydrofolate cyclo-ligase (5-FCL) No genes
5-formyltetrahydrofolate cyclo-ligase (EC 6.3.3.2) SMc02594 +0.2
  SMc03974 -4.0
Acetoacetyl-CoA reductase (EC 1.1.1.36) SMc03878 -0.9
Acetyl-CoA acetyltransferase (EC 2.3.1.9) SMc03879 -5.1
Aconitate hydratase (EC 4.2.1.3) SMc03846 N.D.
Citrate synthase (EC 2.3.3.1) No genes
Citrate synthase (si) (EC 2.3.3.1) SMc02087 N.D.
Enolase (EC 4.2.1.11) SMc01028 -6.9
Formate--tetrahydrofolate ligase (EC 6.3.4.3) SMc02728 -0.0
Formiminotetrahydrofolate cyclodeaminase (EC 4.3.1.4) No genes
Fumarate hydratase class I, aerobic (EC 4.2.1.2) No genes
Fumarate hydratase class I, anaerobic (EC 4.2.1.2) No genes
GDSL-like lipase/acylhydrolase No genes
Glycerate kinase (EC 2.7.1.31) No genes
Isocitrate lyase (EC 4.1.3.1) SMc00768 -4.4
Malate dehydrogenase (EC 1.1.1.37) SMc02479 N.D.
Malate synthase-related protein No genes
Malyl-CoA lyase (EC 4.1.3.24) No genes
Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9) SMc02604 +0.2
  SMc03059 N.D.
Methylcrotonyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.4) SM_b21124 +0.2
Methylcrotonyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.4) SM_b21122 +0.2
Methylene tetrahydromethanopterin dehydrogenase (EC 1.5.99.9) No genes
Methylenetetrahydrofolate dehydrogenase (NADP+) (EC 1.5.1.5) SMc02604 +0.2
  SMc03059 N.D.
Methylmalonyl-CoA mutase (EC 5.4.99.2) SM_b20757 +0.2
N(5),N(10)-methenyltetrahydromethanopterin cyclohydrolase (EC 3.5.4.27) No genes
Phosphoenolpyruvate carboxykinase [ATP] (EC 4.1.1.49) SMc02562 -5.9
Phosphoglyceromutase No genes
Propionyl-CoA carboxylase beta chain (EC 6.4.1.3) No genes
Propionyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.3) SM_b20755 +0.1
Putative malate dehydrogenase (EC 1.1.1.37), similar to archaeal MJ1425 No genes
Serine hydroxymethyltransferase (EC 2.1.2.1) SMa2135 -0.1
  SMc01770 -3.6
Serine--glyoxylate aminotransferase (EC 2.6.1.45) SMa2139 +0.0
Serine--pyruvate aminotransferase (EC 2.6.1.51) SMa1495 +0.2
Serine-pyruvate aminotransferase/archaeal aspartate aminotransferase No genes
Succinate dehydrogenase flavoprotein subunit (EC 1.3.99.1) SMc02349 -0.2
  SMc02465 N.D.
Succinate dehydrogenase iron-sulfur protein (EC 1.3.99.1) SMc02466 N.D.
Succinyl-CoA ligase [ADP-forming] alpha chain (EC 6.2.1.5) SMc02481 N.D.
Succinyl-CoA ligase [ADP-forming] beta chain (EC 6.2.1.5) SMc02480 N.D.
Succinyl-CoA:3-ketoacid-coenzyme A transferase subunit A (EC 2.8.3.5) No genes
Succinyl-CoA:3-ketoacid-coenzyme A transferase subunit B (EC 2.8.3.5) No genes
crotonyl-CoA reductase No genes
cytosolic long-chain acyl-CoA thioester hydrolase family protein SM_b21634 -0.3
  SMc04228 -0.3
decarboxylase No genes
isobutyryl-CoA dehydrogenase No genes
low-specificity D-threonine aldolase SM_b21134 -0.1
polyhydroxyalkanoate synthesis repressor PhaR No genes
putative glycerate kinase No genes
putative isobutyryl-CoA mutase, chain B No genes

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