SEED Subsystem: Nitrogen fixation in Klebsiella michiganensis M5al

Experiments:

Role Gene Name RCH2_defined_Glucose_noNitrogen in Eppendorf tube  
⨯
4Fe-4S ferredoxin, nitrogenase-associated No genes
AnfO protein, required for Mo- and V-independent nitrogenase No genes
AnfR protein, required for Mo- and V-independent nitrogenase No genes
Cysteine desulfurase (EC 2.8.1.7), NifS subfamily BWI76_RS18855 -0.9
Homocitrate synthase (EC 2.3.3.14) BWI76_RS18860 -1.1
Homocitrate synthase alpha subunit (EC 2.3.3.14) No genes
Homocitrate synthase omega subunit (EC 2.3.3.14) No genes
Iron-sulfur cluster assembly scaffold protein NifU BWI76_RS18850 -0.7
LRV (FeS)4 cluster domain protein clustered with nitrogenase cofactor synthesis No genes
NifB-domain protein, type 2 No genes
NifM protein No genes
NifT protein No genes
NifX-associated protein No genes
NifY protein No genes
NifZ protein BWI76_RS18870 -1.6
Nitrogenase (iron-iron) alpha chain (EC 1.18.6.1) No genes
Nitrogenase (iron-iron) beta chain (EC 1.18.6.1) No genes
Nitrogenase (iron-iron) delta chain (EC 1.18.6.1) No genes
Nitrogenase (iron-iron) reductase and maturation protein AnfH No genes
Nitrogenase (iron-iron) transcriptional regulator No genes
Nitrogenase (molybdenum-iron) alpha chain (EC 1.18.6.1) BWI76_RS18815 -1.4
Nitrogenase (molybdenum-iron) beta chain (EC 1.18.6.1) BWI76_RS18820 -1.1
Nitrogenase (molybdenum-iron) reductase and maturation protein NifH BWI76_RS18810 -0.3
Nitrogenase (molybdenum-iron)-specific transcriptional regulator NifA BWI76_RS18890 -1.2
Nitrogenase (vanadium-iron) alpha chain (EC 1.18.6.1) No genes
Nitrogenase (vanadium-iron) beta chain (EC 1.18.6.1) No genes
Nitrogenase (vanadium-iron) delta chain (EC 1.18.6.1) No genes
Nitrogenase (vanadium-iron) reductase and maturation protein VnfH No genes
Nitrogenase (vanadium-iron) transcriptional regulator VnfA No genes
Nitrogenase FeMo-cofactor carrier protein NifX BWI76_RS18830 -0.6
  BWI76_RS18845 -0.2
Nitrogenase FeMo-cofactor scaffold and assembly protein NifE BWI76_RS18835 -1.5
Nitrogenase FeMo-cofactor scaffold and assembly protein NifN BWI76_RS18840 -1.3
Nitrogenase FeMo-cofactor synthesis FeS core scaffold and assembly protein NifB BWI76_RS18895 -1.7
Nitrogenase FeMo-cofactor synthesis molybdenum delivery protein NifQ BWI76_RS18900 -1.2
Nitrogenase cofactor carrier protein NafY No genes
Nitrogenase stabilizing/protective protein NifW No genes
Nitrogenase vanadium-cofactor synthesis protein VnfE No genes
Nitrogenase vanadium-cofactor synthesis protein VnfN No genes
Nitrogenase vanadium-cofactor synthesis protein VnfX No genes
Nitrogenase vanadium-cofactor synthesis protein VnfY No genes
Nitrogenase-associated protein NifO No genes
Transcriptional repressor of nif and glnA operons No genes
probable iron binding protein from the HesB_IscA_SufA family in Nif operon No genes

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