SEED Subsystem: Biotin biosynthesis in Klebsiella michiganensis M5al

Experiments:

Role Gene Name Fungal_growth_media_mCAFEs_v1_noCarbon with Supernatant; Trichoderma virens G41 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.33x; and D-Glucose 74 mM  
3-ketoacyl-CoA thiolase (EC 2.3.1.16) No genes
8-amino-7-oxononanoate synthase (EC 2.3.1.47) BWI76_RS08765 -0.3
ATPase component BioM of energizing module of biotin ECF transporter No genes
Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (EC 2.6.1.62) BWI76_RS07600 +0.2
  BWI76_RS08755 +0.0
Biotin operon repressor BWI76_RS01425 N.D.
Biotin synthase (EC 2.8.1.6) BWI76_RS08760 -1.4
Biotin synthesis protein bioC No genes
Biotin synthesis protein bioG No genes
Biotin synthesis protein bioH No genes
Biotin synthesis protein bioK No genes
Biotin synthesis protein bioZ No genes
Biotin-protein ligase (EC 6.3.4.15) No genes
Dethiobiotin synthetase (EC 6.3.3.3) BWI76_RS08775 +0.1
  BWI76_RS15950 -0.3
Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3) BWI76_RS17800 +0.1
Pimeloyl-CoA synthase (EC 6.2.1.14) No genes
Predicted biotin repressor from TetR family No genes
Substrate-specific component BioY of biotin ECF transporter No genes
Transmembrane component BioN of energizing module of biotin ECF transporter No genes
predicted biotin regulatory protein BioR (GntR family) BWI76_RS10290 +0.0

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