SEED Subsystem: Methionine Biosynthesis in Pseudomonas syringae pv. syringae B728a

Experiments:

Role Gene Name library passed through aphids in food, 72 hours  
⨯
5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20) Psyr_0459 +0.1
5-methyltetrahydrofolate--homocysteine methyltransferase (EC 2.1.1.13) Psyr_2464 -2.0
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (EC 2.1.1.14) Psyr_2855 +0.3
  Psyr_3916 +0.3
Adenosylhomocysteinase (EC 3.3.1.1) Psyr_0460 N.D.
Archaeal S-adenosylmethionine synthetase (EC 2.5.1.6) No genes
Betaine--homocysteine S-methyltransferase (EC 2.1.1.5) No genes
Cystathionine beta-lyase (EC 4.4.1.8) Psyr_0607 -0.0
Cystathionine beta-lyase, type II (EC 4.4.1.8) No genes
Cystathionine beta-synthase (EC 4.2.1.22) No genes
Cystathionine gamma-lyase (EC 4.4.1.1) No genes
Cystathionine gamma-synthase (EC 2.5.1.48) No genes
Cysteine synthase (EC 2.5.1.47) Psyr_1583 +0.2
  Psyr_3697 N.D.
  Psyr_4321 -0.1
Duplicated ATPase component MtsB of energizing module of methionine-regulated ECF transporter No genes
Homocysteine S-methyltransferase (EC 2.1.1.10) Psyr_0975 +0.2
Homoserine O-acetyltransferase (EC 2.3.1.31) Psyr_0474 -0.3
  Psyr_2638 -0.1
Homoserine O-succinyltransferase (EC 2.3.1.46) No genes
Homoserine dehydrogenase (EC 1.1.1.3) Psyr_1290 N.D.
Homoserine kinase (EC 2.7.1.39) Psyr_0272 N.D.
  Psyr_2079 +0.1
Methionine ABC transporter ATP-binding protein Psyr_0281 -0.1
  Psyr_0350 +0.6
  Psyr_4470 -0.5
Methionine ABC transporter permease protein Psyr_0282 -0.2
  Psyr_0349 -0.3
Methionine ABC transporter substrate-binding protein Psyr_0283 +0.3
  Psyr_0328 +0.4
  Psyr_0351 +0.2
Methionine repressor MetJ No genes
Methionine synthase activation domain (EC 2.1.1.13) No genes
Methionine transporter MetT No genes
O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) Psyr_1669 +0.1
O-succinylhomoserine sulfhydrylase (EC 2.5.1.48) Psyr_1669 +0.1
Predicted regulator of methionine metabolism, ArsR family No genes
S-adenosylhomocysteine nucleosidase (EC 3.2.2.9) No genes
S-adenosylmethionine synthetase (EC 2.5.1.6) Psyr_4794 N.D.
S-ribosylhomocysteine lyase (EC 4.4.1.21) No genes
SAM-dependent methlytransferase UbiG No genes
SAM-dependent methlytransferase YrrT No genes
Serine acetyltransferase (EC 2.3.1.30) Psyr_0360 +0.0
  Psyr_1235 -0.2
Substrate-specific component MtsA of methionine-regulated ECF transporter No genes
Transcriptional activator MetR Psyr_3917 +0.1
Transmembrane component MtsC of energizing module of methionine-regulated ECF transporter No genes

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