SEED Subsystem: Serine-glyoxylate cycle in Pseudomonas fluorescens FW300-N2E2

Experiments:

Role Gene Name L-Leucine (C)  
⨯
3-ketoacyl-CoA thiolase (EC 2.3.1.16) Pf6N2E2_1145 N.D.
  Pf6N2E2_2539 +0.0
5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20) Pf6N2E2_4638 -2.0
5-FCL-like protein, but predicted not to be 5-formyltetrahydrofolate cyclo-ligase (5-FCL) No genes
5-formyltetrahydrofolate cyclo-ligase (EC 6.3.3.2) Pf6N2E2_4476 -0.4
Acetoacetyl-CoA reductase (EC 1.1.1.36) No genes
Acetyl-CoA acetyltransferase (EC 2.3.1.9) Pf6N2E2_2113 -0.2
Aconitate hydratase (EC 4.2.1.3) Pf6N2E2_2301 -0.1
Citrate synthase (EC 2.3.3.1) No genes
Citrate synthase (si) (EC 2.3.3.1) Pf6N2E2_5850 N.D.
Enolase (EC 4.2.1.11) Pf6N2E2_3048 N.D.
Formate--tetrahydrofolate ligase (EC 6.3.4.3) No genes
Formiminotetrahydrofolate cyclodeaminase (EC 4.3.1.4) No genes
Fumarate hydratase class I, aerobic (EC 4.2.1.2) Pf6N2E2_5489 N.D.
Fumarate hydratase class I, anaerobic (EC 4.2.1.2) No genes
GDSL-like lipase/acylhydrolase No genes
Glycerate kinase (EC 2.7.1.31) Pf6N2E2_460 +0.4
  Pf6N2E2_5532 N.D.
Isocitrate lyase (EC 4.1.3.1) Pf6N2E2_279 -4.5
Malate dehydrogenase (EC 1.1.1.37) No genes
Malate synthase-related protein No genes
Malyl-CoA lyase (EC 4.1.3.24) No genes
Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9) Pf6N2E2_2221 N.D.
Methylcrotonyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.4) Pf6N2E2_2194 -6.0
  Pf6N2E2_2411 +0.1
Methylcrotonyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.4) Pf6N2E2_2192 -4.9
  Pf6N2E2_2409 -0.1
Methylene tetrahydromethanopterin dehydrogenase (EC 1.5.99.9) No genes
Methylenetetrahydrofolate dehydrogenase (NADP+) (EC 1.5.1.5) Pf6N2E2_2221 N.D.
Methylmalonyl-CoA mutase (EC 5.4.99.2) No genes
N(5),N(10)-methenyltetrahydromethanopterin cyclohydrolase (EC 3.5.4.27) No genes
Phosphoenolpyruvate carboxykinase [ATP] (EC 4.1.1.49) Pf6N2E2_3899 +1.4
Phosphoglyceromutase No genes
Propionyl-CoA carboxylase beta chain (EC 6.4.1.3) No genes
Propionyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.3) No genes
Putative malate dehydrogenase (EC 1.1.1.37), similar to archaeal MJ1425 No genes
Serine hydroxymethyltransferase (EC 2.1.2.1) Pf6N2E2_4717 +0.3
  Pf6N2E2_5082 N.D.
  Pf6N2E2_5083 +0.0
  Pf6N2E2_5295 -0.1
Serine--glyoxylate aminotransferase (EC 2.6.1.45) No genes
Serine--pyruvate aminotransferase (EC 2.6.1.51) No genes
Serine-pyruvate aminotransferase/archaeal aspartate aminotransferase No genes
Succinate dehydrogenase flavoprotein subunit (EC 1.3.99.1) Pf6N2E2_5853 N.D.
Succinate dehydrogenase iron-sulfur protein (EC 1.3.99.1) Pf6N2E2_5854 N.D.
Succinyl-CoA ligase [ADP-forming] alpha chain (EC 6.2.1.5) Pf6N2E2_5859 N.D.
Succinyl-CoA ligase [ADP-forming] beta chain (EC 6.2.1.5) Pf6N2E2_5858 N.D.
Succinyl-CoA:3-ketoacid-coenzyme A transferase subunit A (EC 2.8.3.5) Pf6N2E2_2111 -1.3
Succinyl-CoA:3-ketoacid-coenzyme A transferase subunit B (EC 2.8.3.5) Pf6N2E2_2112 -1.4
crotonyl-CoA reductase No genes
cytosolic long-chain acyl-CoA thioester hydrolase family protein Pf6N2E2_4077 -0.3
  Pf6N2E2_4269 -0.0
  Pf6N2E2_4641 +0.1
decarboxylase Pf6N2E2_2991 +0.0
isobutyryl-CoA dehydrogenase No genes
low-specificity D-threonine aldolase Pf6N2E2_354 +0.0
polyhydroxyalkanoate synthesis repressor PhaR No genes
putative glycerate kinase No genes
putative isobutyryl-CoA mutase, chain B No genes

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