SEED Subsystem: Thiamin biosynthesis in Klebsiella michiganensis M5al

Role	Gene	Name	Mix of RCH2_defined_noCarbon and Fungal_growth_media_mCAFEs_v1_noCarbon; D-Glucose at 55.5 mM
1-deoxy-D-xylulose 5-phosphate synthase (EC 2.2.1.7)	BWI76_RS06380		N.D.
5-FCL-like protein	No genes
ATPase component YkoD of energizing module of thiamin-regulated ECF transporter for HydroxyMethylPyrimidine	No genes
Additional substrate-binding component of thiamin-regulated ECF transporter for HydroxyMethylPyrimidine	No genes
Cysteine desulfurase (EC 2.8.1.7), IscS subfamily	BWI76_RS21160		N.D.
Glycine oxidase ThiO (EC 1.4.3.19)	No genes
Hydroxyethylthiazole kinase (EC 2.7.1.50)	BWI76_RS19380		-2.0
Hydroxymethylpyrimidine ABC transporter, ATPase component	No genes
Hydroxymethylpyrimidine ABC transporter, substrate-binding component	BWI76_RS14025		+0.1
Hydroxymethylpyrimidine ABC transporter, transmembrane component	BWI76_RS14030		+0.3
Hydroxymethylpyrimidine synthesis protein THI5	No genes
Phosphomethylpyrimidine kinase (EC 2.7.4.7)	No genes
Predicted hydroxymethylpyrimidine transporter CytX	No genes
Predicted thiamin transporter PnuT	No genes
Predicted thiamin transporter ThiT	No genes
Predicted thiazole transporter ThiU	No genes
Putative cysteine desulfurase, associated with tRNA 4-thiouridine synthase	No genes
Rhodanese-like domain required for thiamine synthesis	No genes
Substrate-specific component ThiT of thiamin ECF transporter	No genes
Substrate-specific component ThiW of predicted thiazole ECF transporter	No genes
Substrate-specific component YkoE of thiamin-regulated ECF transporter for HydroxyMethylPyrimidine	No genes
Sulfur carrier protein ThiS	BWI76_RS01540		-3.0
Sulfur carrier protein adenylyltransferase ThiF	BWI76_RS01545		-1.8
Thiamin ABC transporter, ATPase component	BWI76_RS04605		-0.1
Thiamin ABC transporter, substrate-binding component	BWI76_RS04615		-0.8
Thiamin ABC transporter, transmembrane component	BWI76_RS04610		-0.0
Thiamin biosynthesis protein ThiC	No genes
Thiamin pyrophosphokinase (EC 2.7.6.2)	No genes
Thiamin transport gene THI10	No genes
Thiamin-phosphate phosphatase (EC 3.1.3.-)	No genes
Thiamin-phosphate pyrophosphorylase (EC 2.5.1.3)	BWI76_RS01550		-3.0
Thiamin-phosphate synthase ThiN (EC 2.5.1.3)	No genes
Thiamin-regulated hypothetical deacetylase	No genes
Thiamin-regulated outer membrane receptor Omr1	No genes
Thiaminase II (EC 3.5.99.2)	BWI76_RS14575		+0.1
Thiamine kinase (EC 2.7.1.89)	No genes
Thiamine-monophosphate kinase (EC 2.7.4.16)	BWI76_RS06365		N.D.
Thiazole biosynthesis protein ThiG	BWI76_RS01535		-3.1
Thiazole biosynthesis protein ThiH	No genes
Thiazole biosynthetic enzyme Thi4	No genes
Transmembrane component YkoC of energizing module of thiamin-regulated ECF transporter for HydroxyMethylPyrimidine	No genes
YjbQ (alternate ThiE)	No genes
tRNA S(4)U 4-thiouridine synthase (former ThiI)	No genes