SEED Subsystem: Valine degradation in Pseudomonas fluorescens FW300-N2E2

Experiments:

Role Gene Name L-Isoleucine (N)  
⨯
3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) Pf6N2E2_1833 -0.3
  Pf6N2E2_1850 -0.3
  Pf6N2E2_1922 -0.0
  Pf6N2E2_2290 N.D.
  Pf6N2E2_3894 +0.1
  Pf6N2E2_6000 -0.0
3-hydroxyisobutyrate dehydrogenase (EC 1.1.1.31) Pf6N2E2_3451 -0.4
3-hydroxyisobutyryl-CoA hydrolase (EC 3.1.2.4) Pf6N2E2_1149 +0.1
Branched-chain acyl-CoA dehydrogenase (EC 1.3.99.12) No genes
Branched-chain alpha-keto acid dehydrogenase, E1 component, alpha subunit (EC 1.2.4.4) Pf6N2E2_481 -2.2
Branched-chain alpha-keto acid dehydrogenase, E1 component, beta subunit (EC 1.2.4.4) Pf6N2E2_480 -1.7
Branched-chain amino acid aminotransferase (EC 2.6.1.42) Pf6N2E2_477 -5.0
Butyrate kinase (EC 2.7.2.7) No genes
Butyryl-CoA dehydrogenase (EC 1.3.99.2) Pf6N2E2_1022 +0.6
  Pf6N2E2_1146 -1.7
  Pf6N2E2_1148 +0.1
  Pf6N2E2_1855 -0.2
  Pf6N2E2_5333 -0.1
  Pf6N2E2_5924 +0.1
Dihydrolipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex (EC 2.3.1.168) Pf6N2E2_479 -1.3
Dihydrolipoamide dehydrogenase of branched-chain alpha-keto acid dehydrogenase (EC 1.8.1.4) Pf6N2E2_478 -1.0
Enoyl-CoA hydratase (EC 4.2.1.17) Pf6N2E2_1013 -0.5
  Pf6N2E2_1046 -0.6
  Pf6N2E2_1834 -0.8
  Pf6N2E2_1835 -0.1
  Pf6N2E2_1840 +0.1
  Pf6N2E2_1922 -0.0
  Pf6N2E2_1934 -0.1
  Pf6N2E2_1935 -0.6
  Pf6N2E2_2290 N.D.
  Pf6N2E2_2773 +0.0
  Pf6N2E2_3894 +0.1
  Pf6N2E2_5493 -0.0
Enoyl-CoA hydratase [valine degradation] (EC 4.2.1.17) Pf6N2E2_1147 -3.3
  Pf6N2E2_1269 +0.0
Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex (EC 2.3.1.-) No genes
Methylmalonate-semialdehyde dehydrogenase (EC 1.2.1.27) Pf6N2E2_3452 -0.0
  Pf6N2E2_3462 -0.2
Predicted transcriptional regulator of leucine degradation pathway, MerR family No genes
Probable acyl-CoA dehydrogenase (EC 1.3.99.3) Pf6N2E2_1144 N.D.
Transcriptional regulator BkdR of isoleucine and valine catabolism operon Pf6N2E2_482 -2.2
phosphotransbutyrylase (EC 2.3.1.19) No genes

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