SEED Subsystem: Cysteine Biosynthesis in Klebsiella michiganensis M5al

Experiments:

Role Gene Name Cobalt chloride 0.5 mM  
⨯
Adenylylsulfate kinase (EC 2.7.1.25) BWI76_RS22690 +0.8
Cys regulon transcriptional activator CysB BWI76_RS11930 N.D.
Cystathionine beta-synthase (EC 4.2.1.22) BWI76_RS24205 -0.1
Cystathionine gamma-lyase (EC 4.4.1.1) BWI76_RS24210 +0.3
Cysteine synthase (EC 2.5.1.47) BWI76_RS20640 +0.2
Cysteine synthase A (EC 2.5.1.47) No genes
Cysteine synthase B (EC 2.5.1.47) BWI76_RS06575 +1.0
  BWI76_RS20685 N.D.
Phosphoadenylyl-sulfate reductase [thioredoxin] (EC 1.8.4.8) BWI76_RS22755 +0.5
Serine acetyltransferase (EC 2.3.1.30) BWI76_RS05560 N.D.
  BWI76_RS27205 N.D.
Sulfate adenylyltransferase subunit 1 (EC 2.7.7.4) BWI76_RS22695 -0.5
Sulfate adenylyltransferase subunit 2 (EC 2.7.7.4) BWI76_RS22700 +0.2
Sulfate and thiosulfate binding protein CysP BWI76_RS20705 +2.0
Sulfate and thiosulfate import ATP-binding protein CysA (EC 3.6.3.25) BWI76_RS20690 +2.0
Sulfate permease BWI76_RS19875 N.D.
  BWI76_RS21805 +0.0
Sulfate permease, Trk-type No genes
Sulfate transport system permease protein CysT BWI76_RS20700 +2.0
Sulfate transport system permease protein CysW BWI76_RS20695 +2.4
Sulfate transporter, CysZ-type BWI76_RS20635 +0.0
Sulfite reductase [NADPH] flavoprotein alpha-component (EC 1.8.1.2) BWI76_RS22765 +0.1
Sulfite reductase [NADPH] hemoprotein beta-component (EC 1.8.1.2) BWI76_RS22760 +0.2

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