SEED Subsystem: Thiamin biosynthesis in Herbaspirillum seropedicae SmR1

Experiments:

Role Gene Name L-Malic (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic acid; 8 days  
1-deoxy-D-xylulose 5-phosphate synthase (EC 2.2.1.7) HSERO_RS19125 N.D.
5-FCL-like protein No genes
ATPase component YkoD of energizing module of thiamin-regulated ECF transporter for HydroxyMethylPyrimidine No genes
Additional substrate-binding component of thiamin-regulated ECF transporter for HydroxyMethylPyrimidine No genes
Cysteine desulfurase (EC 2.8.1.7), IscS subfamily HSERO_RS14180 -1.2
  HSERO_RS15770 N.D.
Glycine oxidase ThiO (EC 1.4.3.19) No genes
Hydroxyethylthiazole kinase (EC 2.7.1.50) No genes
Hydroxymethylpyrimidine ABC transporter, ATPase component HSERO_RS21300 +0.0
Hydroxymethylpyrimidine ABC transporter, substrate-binding component HSERO_RS02285 -0.0
  HSERO_RS16650 -0.1
  HSERO_RS18345 -0.2
Hydroxymethylpyrimidine ABC transporter, transmembrane component HSERO_RS02305 -0.0
  HSERO_RS21305 -0.1
Hydroxymethylpyrimidine synthesis protein THI5 No genes
Phosphomethylpyrimidine kinase (EC 2.7.4.7) No genes
Predicted hydroxymethylpyrimidine transporter CytX No genes
Predicted thiamin transporter PnuT No genes
Predicted thiamin transporter ThiT No genes
Predicted thiazole transporter ThiU No genes
Putative cysteine desulfurase, associated with tRNA 4-thiouridine synthase No genes
Rhodanese-like domain required for thiamine synthesis No genes
Substrate-specific component ThiT of thiamin ECF transporter No genes
Substrate-specific component ThiW of predicted thiazole ECF transporter No genes
Substrate-specific component YkoE of thiamin-regulated ECF transporter for HydroxyMethylPyrimidine No genes
Sulfur carrier protein ThiS HSERO_RS02125 +0.1
Sulfur carrier protein adenylyltransferase ThiF HSERO_RS00835 -0.3
  HSERO_RS02430 +0.4
Thiamin ABC transporter, ATPase component No genes
Thiamin ABC transporter, substrate-binding component No genes
Thiamin ABC transporter, transmembrane component No genes
Thiamin biosynthesis protein ThiC No genes
Thiamin pyrophosphokinase (EC 2.7.6.2) No genes
Thiamin transport gene THI10 No genes
Thiamin-phosphate phosphatase (EC 3.1.3.-) No genes
Thiamin-phosphate pyrophosphorylase (EC 2.5.1.3) HSERO_RS02140 -0.1
Thiamin-phosphate synthase ThiN (EC 2.5.1.3) No genes
Thiamin-regulated hypothetical deacetylase No genes
Thiamin-regulated outer membrane receptor Omr1 No genes
Thiaminase II (EC 3.5.99.2) No genes
Thiamine kinase (EC 2.7.1.89) No genes
Thiamine-monophosphate kinase (EC 2.7.4.16) HSERO_RS16830 N.D.
Thiazole biosynthesis protein ThiG HSERO_RS02130 +0.3
Thiazole biosynthesis protein ThiH No genes
Thiazole biosynthetic enzyme Thi4 No genes
Transmembrane component YkoC of energizing module of thiamin-regulated ECF transporter for HydroxyMethylPyrimidine No genes
YjbQ (alternate ThiE) No genes
tRNA S(4)U 4-thiouridine synthase (former ThiI) No genes

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