SEED Subsystem: Queuosine-Archaeosine Biosynthesis in Klebsiella michiganensis M5al

Experiments:

Role Gene Name Paraquat dichloride 0.1 mg/ml  
COG2047: Uncharacterized protein (ATP-grasp superfamily) No genes
Dihydroneopterin triphosphate pyrophosphohydolase No genes
Dihydroneopterin triphosphate pyrophosphohydolase type 2 BWI76_RS18165 -1.0
GTP cyclohydrolase I (EC 3.5.4.16) type 1 BWI76_RS19660 N.D.
GTP cyclohydrolase I (EC 3.5.4.16) type 2 No genes
Glutamine amidotransferases class-II (GATase) No genes
Hydrolase (HAD superfamily) in cluster with DUF1447 No genes
Inosine-uridine preferring nucleoside hydrolase (EC 3.2.2.1) BWI76_RS04360 +0.4
  BWI76_RS13490 +0.1
NADPH dependent preQ0 reductase No genes
NADPH dependent preQ0 reductase-like No genes
Peptidyl-prolyl cis-trans isomerase (EC 5.2.1.8) No genes
Peptidyl-prolyl cis-trans isomerase ppiB (EC 5.2.1.8) No genes
Permease of the drug/metabolite transporter (DMT) superfamily BWI76_RS02680 -0.2
  BWI76_RS07555 -0.2
  BWI76_RS10350 +0.0
  BWI76_RS13350 -0.0
  BWI76_RS13725 -0.3
  BWI76_RS15305 +0.4
  BWI76_RS18410 +0.4
  BWI76_RS23525 -0.1
  BWI76_RS25175 -0.4
  BWI76_RS27650 -0.0
Putative preQ0 transporter BWI76_RS26435 +1.6
Queuosine Biosynthesis QueC ATPase BWI76_RS06560 -0.8
Queuosine Biosynthesis QueE Radical SAM BWI76_RS22780 -0.2
Queuosine biosynthesis QueD, PTPS-I No genes
Radical SAM family enzyme, similar to coproporphyrinogen III oxidase, oxygen-independent, clustered with nucleoside-triphosphatase RdgB BWI76_RS24120 +0.5
S-adenosylmethionine:tRNA ribosyltransferase-isomerase (EC 5.-.-.-) BWI76_RS06295 +2.5
Substrate-specific component QueT (COG4708) of predicted queuosine-regulated ECF transporter No genes
Substrate-specific component STY3230 of queuosine-regulated ECF transporter BWI76_RS23995 N.D.
archaeosine tRNA-ribosyltransferase (EC 2.4.2.-) type 1 No genes
archaeosine tRNA-ribosyltransferase (EC 2.4.2.-) type 1 like No genes
archaeosine tRNA-ribosyltransferase (EC 2.4.2.-) type 2 No genes
archaeosine tRNA-ribosyltransferase (EC 2.4.2.-) type 5 No genes
glutamyl-Q-tRNA synthetase BWI76_RS05010 +0.4
preQ1-regulated inosine-uridine nucleoside hydrolase (EC 3.2.2.1) No genes
tRNA-guanine transglycosylase (EC 2.4.2.29) BWI76_RS06300 N.D.

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