SEED Subsystem: Polyhydroxybutyrate metabolism in Pseudomonas fluorescens FW300-N2E3

Experiments:

Role Gene Name 4C survival; 360 hrs  
⨯
3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) AO353_00510 -1.3
  AO353_01155 -0.1
3-hydroxybutyryl-CoA dehydratase (EC 4.2.1.55) No genes
3-hydroxybutyryl-CoA dehydrogenase (EC 1.1.1.157) AO353_27535 -0.0
3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3) AO353_00510 -1.3
  AO353_01155 -0.1
3-ketoacyl-CoA thiolase (EC 2.3.1.16) AO353_05785 +0.0
  AO353_25685 +1.2
Acetoacetyl-CoA reductase (EC 1.1.1.36) AO353_01060 +2.4
Acetoacetyl-CoA synthetase (EC 6.2.1.16) AO353_02215 +0.0
  AO353_20355 -0.2
  AO353_21765 +0.1
Acetyl-CoA acetyltransferase (EC 2.3.1.9) AO353_27945 +0.3
Butyrate-acetoacetate CoA-transferase subunit A (EC 2.8.3.9) No genes
Butyrate-acetoacetate CoA-transferase subunit B (EC 2.8.3.9) No genes
D(-)-3-hydroxybutyrate oligomer hydrolase (EC 3.1.1.22) No genes
D-beta-hydroxybutyrate dehydrogenase (EC 1.1.1.30) AO353_21770 +0.1
D-beta-hydroxybutyrate permease AO353_21775 -0.2
Enoyl-CoA hydratase (EC 4.2.1.17) AO353_00510 -1.3
  AO353_03780 -0.2
  AO353_17555 -0.1
  AO353_19125 N.D.
  AO353_23055 -0.0
  AO353_24830 +0.2
Intracellular PHB depolymerase (EC 3.1.1.-) No genes
Poly(3-hydroxyalkanoate) depolymerase AO353_12410 -0.0
Polyhydroxyalkanoate granule-associated protein PhaF AO353_12395 -1.0
Polyhydroxyalkanoic acid synthase AO353_01070 +2.2
  AO353_12405 +0.3
  AO353_12415 +0.3
Putative 3-hydroxyacyl-coa dehydrogenase No genes
Short chain fatty acids transporter AO353_14615 -0.1
polyhydroxyalkanoate granule-associated protein PhaI AO353_12390 -0.8
polyhydroxyalkanoate synthesis repressor PhaR No genes

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