SEED Subsystem: DNA-replication in Pseudomonas fluorescens SBW25-INTG

Experiments:

Role Gene Name Temperature shift 30_to_34; with MOPS  
⨯
ATP-dependent DNA helicase RecG (EC 3.6.1.-) PFLU_RS29550 -0.5
ATP-dependent DNA helicase RecQ PFLU_RS21920 +0.2
Arrested fork binding No genes
Chromosomal replication initiator protein DnaA PFLU_RS00005 N.D.
  PFLU_RS22050 N.D.
Crossover junction endodeoxyribonuclease RuvC (EC 3.1.22.4) PFLU_RS24095 -2.1
DNA polymerase I (EC 2.7.7.7) PFLU_RS00395 -0.7
DNA polymerase II (EC 2.7.7.7) PFLU_RS10185 -0.0
DNA polymerase III alpha subunit (EC 2.7.7.7) PFLU_RS06350 N.D.
  PFLU_RS26455 +0.0
DNA polymerase III beta subunit (EC 2.7.7.7) PFLU_RS00010 N.D.
DNA polymerase III chi subunit (EC 2.7.7.7) PFLU_RS05230 -4.1
DNA polymerase III delta prime subunit (EC 2.7.7.7) PFLU_RS23025 N.D.
DNA polymerase III delta subunit (EC 2.7.7.7) PFLU_RS26600 N.D.
DNA polymerase III epsilon subunit (EC 2.7.7.7) PFLU_RS12925 N.D.
  PFLU_RS27700 -0.1
DNA polymerase III psi subunit (EC 2.7.7.7) No genes
DNA polymerase III subunits gamma and tau (EC 2.7.7.7) PFLU_RS22430 N.D.
DNA polymerase III theta subunit (EC 2.7.7.7) No genes
DNA primase (EC 2.7.7.-) PFLU_RS27530 N.D.
DNA primase (EC 2.7.7.-), phage-associated No genes
DNA primase TraC (EC 2.7.7.-) No genes
DNA repair protein RecN PFLU_RS25910 -0.2
DNA replication protein DnaC No genes
DNA replication terminus site-binding protein No genes
Exodeoxyribonuclease V alpha chain (EC 3.1.11.5) PFLU_RS25585 -1.7
Exodeoxyribonuclease V beta chain (EC 3.1.11.5) PFLU_RS25590 N.D.
Exodeoxyribonuclease V gamma chain (EC 3.1.11.5) PFLU_RS25595 -1.4
Helicase PriA essential for oriC/DnaA-independent DNA replication PFLU_RS01980 N.D.
Helicase loader DnaI No genes
Holliday junction DNA helicase RuvA PFLU_RS24090 -1.6
Holliday junction DNA helicase RuvB PFLU_RS24085 -1.3
Primosomal protein I PFLU_RS13905 +0.0
Primosomal replication protein N No genes
Primosomal replication protein N prime prime No genes
RecA protein PFLU_RS05885 -0.9
Recombination protein RecR PFLU_RS22415 -0.2
Replicative DNA helicase (EC 3.6.1.-) PFLU_RS02655 N.D.
Replicative DNA helicase (EC 3.6.1.-) [SA14-24] No genes
Single-stranded-DNA-specific exonuclease RecJ (EC 3.1.-.-) PFLU_RS24515 -0.1
Transcription-repair coupling factor PFLU_RS07710 +0.2

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