SEED Subsystem: Queuosine-Archaeosine Biosynthesis in Methanococcus maripaludis S2

Experiments:

Role Gene Name H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE  
⨯
COG2047: Uncharacterized protein (ATP-grasp superfamily) MMP_RS04745 +0.2
  MMP_RS08280 +0.2
Dihydroneopterin triphosphate pyrophosphohydolase No genes
Dihydroneopterin triphosphate pyrophosphohydolase type 2 No genes
GTP cyclohydrolase I (EC 3.5.4.16) type 1 No genes
GTP cyclohydrolase I (EC 3.5.4.16) type 2 MMP_RS00220 N.D.
Glutamine amidotransferases class-II (GATase) No genes
Hydrolase (HAD superfamily) in cluster with DUF1447 No genes
Inosine-uridine preferring nucleoside hydrolase (EC 3.2.2.1) No genes
NADPH dependent preQ0 reductase No genes
NADPH dependent preQ0 reductase-like No genes
Peptidyl-prolyl cis-trans isomerase (EC 5.2.1.8) No genes
Peptidyl-prolyl cis-trans isomerase ppiB (EC 5.2.1.8) No genes
Permease of the drug/metabolite transporter (DMT) superfamily MMP_RS01160 +0.1
  MMP_RS02920 +0.2
  MMP_RS03515 +0.1
  MMP_RS08160 +0.3
Putative preQ0 transporter MMP_RS04220 +0.1
Queuosine Biosynthesis QueC ATPase MMP_RS01240 -0.7
Queuosine Biosynthesis QueE Radical SAM MMP_RS00445 -0.7
Queuosine biosynthesis QueD, PTPS-I MMP_RS06430 -0.6
Radical SAM family enzyme, similar to coproporphyrinogen III oxidase, oxygen-independent, clustered with nucleoside-triphosphatase RdgB No genes
S-adenosylmethionine:tRNA ribosyltransferase-isomerase (EC 5.-.-.-) No genes
Substrate-specific component QueT (COG4708) of predicted queuosine-regulated ECF transporter No genes
Substrate-specific component STY3230 of queuosine-regulated ECF transporter MMP_RS00625 +0.2
  MMP_RS00630 -0.2
  MMP_RS02215 +0.2
  MMP_RS03575 -0.0
archaeosine tRNA-ribosyltransferase (EC 2.4.2.-) type 1 MMP_RS03210 -2.4
archaeosine tRNA-ribosyltransferase (EC 2.4.2.-) type 1 like No genes
archaeosine tRNA-ribosyltransferase (EC 2.4.2.-) type 2 MMP_RS08200 -4.0
archaeosine tRNA-ribosyltransferase (EC 2.4.2.-) type 5 No genes
glutamyl-Q-tRNA synthetase No genes
preQ1-regulated inosine-uridine nucleoside hydrolase (EC 3.2.2.1) No genes
tRNA-guanine transglycosylase (EC 2.4.2.29) No genes

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