SEED Subsystem: Queuosine-Archaeosine Biosynthesis in Pseudomonas fluorescens FW300-N2E2

Experiments:

Role Gene Name Doxycycline hyclate 0.0001 mg/ml  
COG2047: Uncharacterized protein (ATP-grasp superfamily) No genes
Dihydroneopterin triphosphate pyrophosphohydolase No genes
Dihydroneopterin triphosphate pyrophosphohydolase type 2 No genes
GTP cyclohydrolase I (EC 3.5.4.16) type 1 Pf6N2E2_5273 -0.2
  Pf6N2E2_5815 N.D.
GTP cyclohydrolase I (EC 3.5.4.16) type 2 Pf6N2E2_4262 +0.1
Glutamine amidotransferases class-II (GATase) No genes
Hydrolase (HAD superfamily) in cluster with DUF1447 No genes
Inosine-uridine preferring nucleoside hydrolase (EC 3.2.2.1) Pf6N2E2_167 -0.2
NADPH dependent preQ0 reductase No genes
NADPH dependent preQ0 reductase-like No genes
Peptidyl-prolyl cis-trans isomerase (EC 5.2.1.8) No genes
Peptidyl-prolyl cis-trans isomerase ppiB (EC 5.2.1.8) No genes
Permease of the drug/metabolite transporter (DMT) superfamily Pf6N2E2_1066 -0.1
  Pf6N2E2_1186 +0.0
  Pf6N2E2_1547 +0.1
  Pf6N2E2_1889 +0.1
  Pf6N2E2_2640 -0.6
  Pf6N2E2_2697 +0.1
  Pf6N2E2_2698 +0.1
  Pf6N2E2_409 -0.0
  Pf6N2E2_4358 -0.1
  Pf6N2E2_455 -0.1
  Pf6N2E2_476 +0.3
  Pf6N2E2_5045 -0.0
  Pf6N2E2_5205 -0.0
  Pf6N2E2_5309 -0.1
  Pf6N2E2_540 +0.1
  Pf6N2E2_6008 -0.1
Putative preQ0 transporter Pf6N2E2_5445 -0.1
Queuosine Biosynthesis QueC ATPase Pf6N2E2_3393 +0.1
  Pf6N2E2_3734 +0.0
  Pf6N2E2_5546 +2.0
Queuosine Biosynthesis QueE Radical SAM Pf6N2E2_5545 +2.3
Queuosine biosynthesis QueD, PTPS-I No genes
Radical SAM family enzyme, similar to coproporphyrinogen III oxidase, oxygen-independent, clustered with nucleoside-triphosphatase RdgB Pf6N2E2_4593 +1.1
S-adenosylmethionine:tRNA ribosyltransferase-isomerase (EC 5.-.-.-) Pf6N2E2_3199 +2.8
Substrate-specific component QueT (COG4708) of predicted queuosine-regulated ECF transporter No genes
Substrate-specific component STY3230 of queuosine-regulated ECF transporter No genes
archaeosine tRNA-ribosyltransferase (EC 2.4.2.-) type 1 No genes
archaeosine tRNA-ribosyltransferase (EC 2.4.2.-) type 1 like No genes
archaeosine tRNA-ribosyltransferase (EC 2.4.2.-) type 2 No genes
archaeosine tRNA-ribosyltransferase (EC 2.4.2.-) type 5 No genes
glutamyl-Q-tRNA synthetase Pf6N2E2_5159 +0.1
preQ1-regulated inosine-uridine nucleoside hydrolase (EC 3.2.2.1) No genes
tRNA-guanine transglycosylase (EC 2.4.2.29) Pf6N2E2_3198 +3.9

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