SEED Subsystem: Peptidoglycan Biosynthesis in Pseudomonas fluorescens FW300-N2E2

Experiments:

Role Gene Name Vancomycin 0.24 mg/ml  
Cell division protein FtsI [Peptidoglycan synthetase] (EC 2.4.1.129) Pf6N2E2_5350 N.D.
D-alanine--D-alanine ligase (EC 6.3.2.4) Pf6N2E2_5358 N.D.
D-alanine--D-alanine ligase A (EC 6.3.2.4) No genes
D-alanine--D-alanine ligase B (EC 6.3.2.4) No genes
D-alanyl-D-alanine carboxypeptidase (EC 3.4.16.4) Pf6N2E2_2344 +2.6
  Pf6N2E2_5001 +0.5
Glucosamine-1-phosphate N-acetyltransferase (EC 2.3.1.157) Pf6N2E2_4215 N.D.
Glutamate racemase (EC 5.1.1.3) Pf6N2E2_5224 N.D.
Glutamine synthetase type I (EC 6.3.1.2) Pf6N2E2_3821 N.D.
Glutamine synthetase type II, eukaryotic (EC 6.3.1.2) No genes
Glutamine synthetase type III, GlnN (EC 6.3.1.2) No genes
Glutamine synthetase, clostridia type (EC 6.3.1.2) No genes
Membrane-bound lytic murein transglycosylase B (EC 3.2.1.-) No genes
Membrane-bound lytic murein transglycosylase B precursor (EC 3.2.1.-) Pf6N2E2_4999 -0.3
  Pf6N2E2_5007 +0.5
Monofunctional biosynthetic peptidoglycan transglycosylase (EC 2.4.2.-) Pf6N2E2_4587 +0.6
Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-) Pf6N2E2_3761 -1.1
  Pf6N2E2_5171 +0.1
Murein-DD-endopeptidase (EC 3.4.99.-) Pf6N2E2_2222 -0.9
N-acetylglucosamine-1-phosphate uridyltransferase (EC 2.7.7.23) Pf6N2E2_4215 N.D.
N-acetylglucosamine-1-phosphate uridyltransferase eukaryotic (EC 2.7.7.23) No genes
Penicillin-binding protein 2 (PBP-2) Pf6N2E2_4997 N.D.
Penicillin-binding protein 4 precursor No genes
Penicillin-binding protein PBP2a, methicillin resistance determinant MecA, transpeptidase No genes
Penicillin-insensitive transglycosylase (EC 2.4.2.-) & transpeptidase PBP-1C No genes
Phospho-N-acetylmuramoyl-pentapeptide-transferase (EC 2.7.8.13) Pf6N2E2_5353 N.D.
Putative glutamine synthetase, Rickettsiales type (EC 6.3.1.2) No genes
Rare lipoprotein A precursor Pf6N2E2_5000 N.D.
Rod shape-determining protein RodA Pf6N2E2_4998 N.D.
UDP-N-acetylenolpyruvoylglucosamine reductase (EC 1.1.1.158) Pf6N2E2_5937 N.D.
UDP-N-acetylglucosamine 1-carboxyvinyltransferase (EC 2.5.1.7) Pf6N2E2_3254 N.D.
UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase (EC 2.4.1.227) Pf6N2E2_5356 N.D.
UDP-N-acetylmuramate--alanine ligase (EC 6.3.2.8) Pf6N2E2_5357 N.D.
UDP-N-acetylmuramoylalanine--D-glutamate ligase (EC 6.3.2.9) Pf6N2E2_5354 N.D.
UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase (EC 6.3.2.13) Pf6N2E2_4015 -0.1
  Pf6N2E2_5351 N.D.
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate--D-alanyl-D-alanine ligase (EC 6.3.2.10) Pf6N2E2_5352 N.D.

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