SEED Subsystem: Serine-glyoxylate cycle in Pseudomonas fluorescens FW300-N1B4

Experiments:

Role Gene Name nitrite 10 mM  
⨯
3-ketoacyl-CoA thiolase (EC 2.3.1.16) Pf1N1B4_371 +0.4
  Pf1N1B4_4786 N.D.
  Pf1N1B4_5589 +0.3
5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20) Pf1N1B4_2384 -1.5
5-FCL-like protein, but predicted not to be 5-formyltetrahydrofolate cyclo-ligase (5-FCL) No genes
5-formyltetrahydrofolate cyclo-ligase (EC 6.3.3.2) Pf1N1B4_2225 -0.1
Acetoacetyl-CoA reductase (EC 1.1.1.36) No genes
Acetyl-CoA acetyltransferase (EC 2.3.1.9) No genes
Aconitate hydratase (EC 4.2.1.3) Pf1N1B4_3888 +0.1
Citrate synthase (EC 2.3.3.1) No genes
Citrate synthase (si) (EC 2.3.3.1) Pf1N1B4_3676 N.D.
Enolase (EC 4.2.1.11) Pf1N1B4_3104 N.D.
Formate--tetrahydrofolate ligase (EC 6.3.4.3) No genes
Formiminotetrahydrofolate cyclodeaminase (EC 4.3.1.4) No genes
Fumarate hydratase class I, aerobic (EC 4.2.1.2) Pf1N1B4_683 -1.8
Fumarate hydratase class I, anaerobic (EC 4.2.1.2) No genes
GDSL-like lipase/acylhydrolase No genes
Glycerate kinase (EC 2.7.1.31) Pf1N1B4_3425 -0.3
  Pf1N1B4_4998 N.D.
  Pf1N1B4_642 +0.2
Isocitrate lyase (EC 4.1.3.1) Pf1N1B4_4042 -0.1
Malate dehydrogenase (EC 1.1.1.37) No genes
Malate synthase-related protein No genes
Malyl-CoA lyase (EC 4.1.3.24) No genes
Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9) Pf1N1B4_3943 N.D.
  Pf1N1B4_5048 N.D.
Methylcrotonyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.4) Pf1N1B4_225 -0.1
  Pf1N1B4_3984 +0.2
Methylcrotonyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.4) Pf1N1B4_222 +0.5
  Pf1N1B4_3986 -0.5
Methylene tetrahydromethanopterin dehydrogenase (EC 1.5.99.9) No genes
Methylenetetrahydrofolate dehydrogenase (NADP+) (EC 1.5.1.5) Pf1N1B4_3943 N.D.
  Pf1N1B4_5048 N.D.
Methylmalonyl-CoA mutase (EC 5.4.99.2) No genes
N(5),N(10)-methenyltetrahydromethanopterin cyclohydrolase (EC 3.5.4.27) No genes
Phosphoenolpyruvate carboxykinase [ATP] (EC 4.1.1.49) Pf1N1B4_1779 N.D.
Phosphoglyceromutase No genes
Propionyl-CoA carboxylase beta chain (EC 6.4.1.3) No genes
Propionyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.3) No genes
Putative malate dehydrogenase (EC 1.1.1.37), similar to archaeal MJ1425 No genes
Serine hydroxymethyltransferase (EC 2.1.2.1) Pf1N1B4_2161 -0.1
  Pf1N1B4_2454 -0.0
  Pf1N1B4_2778 N.D.
  Pf1N1B4_2779 -0.0
Serine--glyoxylate aminotransferase (EC 2.6.1.45) No genes
Serine--pyruvate aminotransferase (EC 2.6.1.51) No genes
Serine-pyruvate aminotransferase/archaeal aspartate aminotransferase Pf1N1B4_5147 -0.1
Succinate dehydrogenase flavoprotein subunit (EC 1.3.99.1) Pf1N1B4_3679 N.D.
Succinate dehydrogenase iron-sulfur protein (EC 1.3.99.1) Pf1N1B4_3680 N.D.
Succinyl-CoA ligase [ADP-forming] alpha chain (EC 6.2.1.5) Pf1N1B4_3685 -0.8
Succinyl-CoA ligase [ADP-forming] beta chain (EC 6.2.1.5) Pf1N1B4_3684 -0.7
Succinyl-CoA:3-ketoacid-coenzyme A transferase subunit A (EC 2.8.3.5) Pf1N1B4_5833 -0.0
Succinyl-CoA:3-ketoacid-coenzyme A transferase subunit B (EC 2.8.3.5) Pf1N1B4_5834 -0.4
crotonyl-CoA reductase No genes
cytosolic long-chain acyl-CoA thioester hydrolase family protein Pf1N1B4_2001 -0.5
  Pf1N1B4_2032 N.D.
  Pf1N1B4_2386 -2.4
decarboxylase No genes
isobutyryl-CoA dehydrogenase No genes
low-specificity D-threonine aldolase Pf1N1B4_4210 -0.0
polyhydroxyalkanoate synthesis repressor PhaR No genes
putative glycerate kinase No genes
putative isobutyryl-CoA mutase, chain B No genes

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